Pseudobutyrivibrio sp. OR37

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Pseudobutyrivibrio; unclassified Pseudobutyrivibrio

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3GLN9|A0A1I3GLN9_9FIRM Type I restriction enzyme S subunit OS=Pseudobutyrivibrio sp. OR37 OX=1798186 GN=SAMN04487830_13326 PE=4 SV=1
MM1 pKa = 6.99QNKK4 pKa = 9.24IVTKK8 pKa = 10.65LLTCALSAGLILSGCGNTSVSSTEE32 pKa = 4.11TEE34 pKa = 4.26VTDD37 pKa = 4.48SANEE41 pKa = 3.92AAGSTTIGQTVSGPARR57 pKa = 11.84DD58 pKa = 4.54DD59 pKa = 3.23ISVNEE64 pKa = 4.3TIEE67 pKa = 4.0NNEE70 pKa = 4.34DD71 pKa = 3.27GEE73 pKa = 4.46HH74 pKa = 6.62AIEE77 pKa = 5.23ASGEE81 pKa = 3.96DD82 pKa = 3.13ASYY85 pKa = 11.84SNVEE89 pKa = 4.2VIKK92 pKa = 10.09TGDD95 pKa = 3.33SDD97 pKa = 3.99EE98 pKa = 4.79GDD100 pKa = 3.38EE101 pKa = 4.97ADD103 pKa = 4.87FYY105 pKa = 11.61GDD107 pKa = 3.1NAAIFATDD115 pKa = 3.59GATLDD120 pKa = 3.62LSEE123 pKa = 5.15IVVDD127 pKa = 4.32TNGTHH132 pKa = 6.62ANDD135 pKa = 3.11VFSYY139 pKa = 11.63GEE141 pKa = 4.15GTTVNISDD149 pKa = 4.63SYY151 pKa = 10.16ITTAGNCSGGLMTTGGGTMNATNLTIEE178 pKa = 4.19TSGNSSAAIRR188 pKa = 11.84SDD190 pKa = 3.19RR191 pKa = 11.84GGGTVTVTGGDD202 pKa = 3.76YY203 pKa = 7.6TTSGTGSPVIYY214 pKa = 9.33STADD218 pKa = 3.25VTVSNATLTSTASQGVVVEE237 pKa = 4.67GNNSATLNNVVLNADD252 pKa = 3.87NNTKK256 pKa = 10.61NSDD259 pKa = 3.12KK260 pKa = 10.85SDD262 pKa = 3.51YY263 pKa = 8.76YY264 pKa = 9.41QAVMIYY270 pKa = 10.65QSMSGDD276 pKa = 3.54ADD278 pKa = 3.46EE279 pKa = 5.1GTASFSLKK287 pKa = 10.65DD288 pKa = 3.55SLLNNANGDD297 pKa = 3.74VFFITNTVADD307 pKa = 3.87INIEE311 pKa = 3.82NSEE314 pKa = 4.18INNNGDD320 pKa = 3.84GILLRR325 pKa = 11.84AAAAGWGNEE334 pKa = 3.95GANGGQVNVNVKK346 pKa = 9.53NQSLEE351 pKa = 3.77GDD353 pKa = 3.55MSIDD357 pKa = 4.08DD358 pKa = 3.86VSAMNLYY365 pKa = 10.32LGEE368 pKa = 4.2GATYY372 pKa = 10.62SGAINTNGEE381 pKa = 3.93AGDD384 pKa = 4.03VYY386 pKa = 10.98IQLTDD391 pKa = 3.37GATWTLTGDD400 pKa = 3.78SYY402 pKa = 11.12VTALACDD409 pKa = 3.73ADD411 pKa = 4.45SINLNGYY418 pKa = 8.34KK419 pKa = 10.22LYY421 pKa = 11.41VNGEE425 pKa = 4.28EE426 pKa = 4.09YY427 pKa = 10.73TEE429 pKa = 4.67GSEE432 pKa = 4.31STGDD436 pKa = 3.9AITLEE441 pKa = 4.54KK442 pKa = 9.8ATSSGAPDD450 pKa = 3.2GGMPEE455 pKa = 4.24GKK457 pKa = 9.97APSGEE462 pKa = 4.15KK463 pKa = 10.38PEE465 pKa = 4.46GNPPSGEE472 pKa = 4.12KK473 pKa = 9.88PAGEE477 pKa = 4.27APSGEE482 pKa = 4.42KK483 pKa = 10.42PEE485 pKa = 4.2GTPPSNEE492 pKa = 3.73NSNKK496 pKa = 9.95NN497 pKa = 3.23

Molecular weight: 50.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3D4E8|A0A1I3D4E8_9FIRM Uncharacterized protein OS=Pseudobutyrivibrio sp. OR37 OX=1798186 GN=SAMN04487830_10943 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.0VHH16 pKa = 5.93GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3127

0

3127

1072131

40

3574

342.9

38.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.493 ± 0.041

1.333 ± 0.019

6.51 ± 0.036

7.364 ± 0.049

4.227 ± 0.033

6.791 ± 0.039

1.592 ± 0.019

7.749 ± 0.046

7.212 ± 0.041

8.302 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.027

5.172 ± 0.036

3.039 ± 0.024

2.72 ± 0.024

3.547 ± 0.032

6.072 ± 0.044

5.648 ± 0.058

6.958 ± 0.031

0.872 ± 0.015

4.504 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski