Mycobacterium phage SkiPole

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; Mycobacterium virus Skipole

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2XRN0|D2XRN0_9CAUD Uncharacterized protein OS=Mycobacterium phage SkiPole OX=701456 GN=36 PE=4 SV=1
MM1 pKa = 7.96SEE3 pKa = 4.32EE4 pKa = 3.94MQQLRR9 pKa = 11.84KK10 pKa = 10.09EE11 pKa = 3.89IFTTYY16 pKa = 10.61VEE18 pKa = 4.35YY19 pKa = 10.84EE20 pKa = 4.01PAGPHH25 pKa = 6.01QGPGWMGFGLNSICWGAWQGWVGNLGGYY53 pKa = 9.1IAVIFYY59 pKa = 10.54NSEE62 pKa = 4.22DD63 pKa = 3.66GSVIAVTLNNFSAHH77 pKa = 6.4ADD79 pKa = 3.56AVDD82 pKa = 4.16LFYY85 pKa = 11.13QIAYY89 pKa = 9.85LLNPEE94 pKa = 4.45STGHH98 pKa = 6.67RR99 pKa = 11.84DD100 pKa = 2.94WIFRR104 pKa = 11.84PDD106 pKa = 3.47PAEE109 pKa = 4.9DD110 pKa = 3.62EE111 pKa = 5.0DD112 pKa = 4.22EE113 pKa = 4.53VRR115 pKa = 11.84DD116 pKa = 3.63PTLYY120 pKa = 9.89LTVEE124 pKa = 4.33STGDD128 pKa = 3.38NQIPADD134 pKa = 3.87VPFEE138 pKa = 3.98II139 pKa = 5.71

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2XRJ7|D2XRJ7_9CAUD Uncharacterized protein OS=Mycobacterium phage SkiPole OX=701456 GN=3 PE=4 SV=1
MM1 pKa = 7.35SWDD4 pKa = 3.28SSDD7 pKa = 3.35RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 4.4RR11 pKa = 11.84LPADD15 pKa = 3.28WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.46RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.29PDD37 pKa = 3.72ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.06VRR50 pKa = 11.84YY51 pKa = 9.51RR52 pKa = 11.84DD53 pKa = 3.5EE54 pKa = 4.68SSPLQAACRR63 pKa = 11.84SCHH66 pKa = 4.77SRR68 pKa = 11.84KK69 pKa = 9.81SAMEE73 pKa = 3.92GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.63PGRR95 pKa = 11.84RR96 pKa = 11.84NKK98 pKa = 10.77EE99 pKa = 3.81EE100 pKa = 3.84TT101 pKa = 3.39

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

16725

32

870

164.0

17.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.961 ± 0.39

0.729 ± 0.129

6.493 ± 0.191

6.547 ± 0.25

3.097 ± 0.164

8.771 ± 0.544

1.931 ± 0.168

4.813 ± 0.217

4.126 ± 0.256

8.478 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.113

3.151 ± 0.155

5.525 ± 0.264

3.175 ± 0.183

6.493 ± 0.401

6.027 ± 0.254

6.38 ± 0.275

7.36 ± 0.185

2.027 ± 0.135

2.762 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski