Panicum streak virus (isolate Kenya) (PanSV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Panicum streak virus

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q00338|REPA_PASVK Replication-associated protein A OS=Panicum streak virus (isolate Kenya) OX=268780 GN=C1 PE=3 SV=1
MM1 pKa = 6.93STVGSSSEE9 pKa = 3.68GRR11 pKa = 11.84HH12 pKa = 4.16SVRR15 pKa = 11.84CFRR18 pKa = 11.84HH19 pKa = 6.14RR20 pKa = 11.84NANTFLTYY28 pKa = 10.26SKK30 pKa = 10.88CPLEE34 pKa = 3.93PEE36 pKa = 4.86FIGEE40 pKa = 3.92HH41 pKa = 6.27LFRR44 pKa = 11.84LTRR47 pKa = 11.84EE48 pKa = 3.93YY49 pKa = 10.61EE50 pKa = 3.57PAYY53 pKa = 9.34ILVVRR58 pKa = 11.84EE59 pKa = 3.9THH61 pKa = 7.15TDD63 pKa = 3.88GTWHH67 pKa = 6.33CHH69 pKa = 6.64ALLQCIKK76 pKa = 10.22PCTTRR81 pKa = 11.84DD82 pKa = 3.17EE83 pKa = 4.79RR84 pKa = 11.84YY85 pKa = 9.6FDD87 pKa = 3.28IDD89 pKa = 3.79RR90 pKa = 11.84YY91 pKa = 10.29HH92 pKa = 7.89GNIQSAKK99 pKa = 8.26STDD102 pKa = 3.09KK103 pKa = 10.55VRR105 pKa = 11.84EE106 pKa = 4.1YY107 pKa = 10.68ILKK110 pKa = 10.31DD111 pKa = 3.71PKK113 pKa = 11.09DD114 pKa = 3.29KK115 pKa = 10.5WEE117 pKa = 4.22KK118 pKa = 8.82GTYY121 pKa = 9.25IPRR124 pKa = 11.84KK125 pKa = 9.51KK126 pKa = 10.78SFVPPGKK133 pKa = 10.29EE134 pKa = 3.49PAEE137 pKa = 4.14KK138 pKa = 10.46KK139 pKa = 7.32PTKK142 pKa = 10.27DD143 pKa = 2.81EE144 pKa = 3.99VMRR147 pKa = 11.84EE148 pKa = 3.94IMTHH152 pKa = 4.33ATSRR156 pKa = 11.84EE157 pKa = 4.06EE158 pKa = 3.93YY159 pKa = 10.39LSLVQSSLPYY169 pKa = 10.68DD170 pKa = 3.27WATKK174 pKa = 10.48LNYY177 pKa = 9.62FEE179 pKa = 5.23YY180 pKa = 10.2SASRR184 pKa = 11.84LFPDD188 pKa = 3.2IAEE191 pKa = 5.11PYY193 pKa = 7.99TNPHH197 pKa = 6.38PTTEE201 pKa = 4.23YY202 pKa = 10.94DD203 pKa = 3.4LHH205 pKa = 7.92CNEE208 pKa = 5.52TIEE211 pKa = 5.55DD212 pKa = 3.55WLKK215 pKa = 10.05PNIYY219 pKa = 9.58QVSPQAYY226 pKa = 8.42KK227 pKa = 10.88LLEE230 pKa = 4.21PSCLSLEE237 pKa = 4.11QAIADD242 pKa = 4.68LEE244 pKa = 4.34WLDD247 pKa = 3.51DD248 pKa = 3.85TTRR251 pKa = 11.84MLQEE255 pKa = 4.21KK256 pKa = 9.35EE257 pKa = 4.3RR258 pKa = 11.84EE259 pKa = 4.18ASTSAAQHH267 pKa = 5.69GQVKK271 pKa = 10.29HH272 pKa = 6.52PGLEE276 pKa = 4.15ASDD279 pKa = 3.77DD280 pKa = 3.82TTTGKK285 pKa = 9.01TISTGLHH292 pKa = 4.59MMKK295 pKa = 10.4KK296 pKa = 10.09LSTMSLTTFPSSSVRR311 pKa = 11.84AGNDD315 pKa = 2.47SSAAKK320 pKa = 8.85KK321 pKa = 6.24TTT323 pKa = 3.14

Molecular weight: 37.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q00336|MP_PASVK Movement protein OS=Panicum streak virus (isolate Kenya) OX=268780 GN=V2 PE=3 SV=1
MM1 pKa = 7.63SGALKK6 pKa = 10.35RR7 pKa = 11.84KK8 pKa = 10.08RR9 pKa = 11.84SDD11 pKa = 2.87EE12 pKa = 4.37VAWSRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84PVKK22 pKa = 10.48KK23 pKa = 9.39PVRR26 pKa = 11.84RR27 pKa = 11.84APPPRR32 pKa = 11.84AGPSVRR38 pKa = 11.84RR39 pKa = 11.84GLPSLQIQTLVAAGDD54 pKa = 3.71TMITVPSGGICSLIGTYY71 pKa = 10.72ARR73 pKa = 11.84GSGEE77 pKa = 4.23GEE79 pKa = 3.64RR80 pKa = 11.84HH81 pKa = 4.78TNEE84 pKa = 3.48TLTYY88 pKa = 10.08KK89 pKa = 10.59VALDD93 pKa = 3.36YY94 pKa = 11.55HH95 pKa = 6.49FVATAAACKK104 pKa = 9.87YY105 pKa = 9.37SSIGIGVCWLVYY117 pKa = 9.92DD118 pKa = 4.45AQPTGTAPTVQDD130 pKa = 4.2IFPHH134 pKa = 6.56PATLSAFPYY143 pKa = 7.05TWKK146 pKa = 10.5VGRR149 pKa = 11.84EE150 pKa = 3.83VCHH153 pKa = 6.21RR154 pKa = 11.84FVVKK158 pKa = 10.44RR159 pKa = 11.84RR160 pKa = 11.84WCFTMEE166 pKa = 3.97TNGRR170 pKa = 11.84IGSDD174 pKa = 3.38TPPSNVAWPPCKK186 pKa = 9.74KK187 pKa = 10.36DD188 pKa = 3.04IYY190 pKa = 10.04FHH192 pKa = 6.77KK193 pKa = 10.19FCTGLGVKK201 pKa = 7.99TEE203 pKa = 4.15WKK205 pKa = 10.42NVTDD209 pKa = 4.62GKK211 pKa = 10.74DD212 pKa = 3.27GAIKK216 pKa = 10.54KK217 pKa = 9.94GGFYY221 pKa = 10.35IVIAPGNVEE230 pKa = 4.4FTCHH234 pKa = 5.16GQCRR238 pKa = 11.84LYY240 pKa = 10.55FKK242 pKa = 10.94SVGNQQ247 pKa = 2.8

Molecular weight: 27.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1037

108

359

259.3

29.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.172 ± 0.662

2.7 ± 0.298

5.207 ± 0.412

6.75 ± 1.225

3.279 ± 0.212

5.786 ± 1.172

2.989 ± 0.56

4.725 ± 0.349

6.847 ± 0.707

7.04 ± 0.837

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.446 ± 0.273

2.797 ± 0.557

7.425 ± 0.94

2.604 ± 0.07

6.75 ± 0.539

7.522 ± 0.699

7.618 ± 1.111

5.4 ± 1.216

2.122 ± 0.224

4.822 ± 0.639
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski