Propionibacteriaceae bacterium NML 030167

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; unclassified Propionibacteriaceae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4158 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255FXJ6|A0A255FXJ6_9ACTN NUDIX hydrolase OS=Propionibacteriaceae bacterium NML 030167 OX=2016500 GN=CGZ94_20865 PE=4 SV=1
MM1 pKa = 7.19AVEE4 pKa = 3.95MSFGEE9 pKa = 4.44FEE11 pKa = 5.19DD12 pKa = 4.56LVADD16 pKa = 5.11ALDD19 pKa = 3.68QVPDD23 pKa = 3.85EE24 pKa = 4.59LVALIDD30 pKa = 3.47NCIVIIEE37 pKa = 4.25EE38 pKa = 4.48EE39 pKa = 4.36PPEE42 pKa = 4.58GDD44 pKa = 3.36PKK46 pKa = 11.37LLGLYY51 pKa = 10.36DD52 pKa = 5.14GIPLTEE58 pKa = 4.39RR59 pKa = 11.84NSDD62 pKa = 3.58YY63 pKa = 11.72TMVLPDD69 pKa = 4.96RR70 pKa = 11.84IFIYY74 pKa = 9.85MNPTLAMCSDD84 pKa = 3.68RR85 pKa = 11.84DD86 pKa = 3.7EE87 pKa = 4.85VVDD90 pKa = 4.15EE91 pKa = 3.9VHH93 pKa = 5.72ITVVHH98 pKa = 6.69EE99 pKa = 4.08IAHH102 pKa = 6.02HH103 pKa = 6.08FGINDD108 pKa = 3.57EE109 pKa = 4.23QLHH112 pKa = 4.82QLGYY116 pKa = 11.03AA117 pKa = 3.57

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255GNQ2|A0A255GNQ2_9ACTN Glyceraldehyde-3-phosphate dehydrogenase OS=Propionibacteriaceae bacterium NML 030167 OX=2016500 GN=gap PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.86GRR40 pKa = 11.84AKK42 pKa = 10.71LAGG45 pKa = 3.63

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4158

0

4158

1373801

33

5305

330.4

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.749 ± 0.045

0.739 ± 0.011

5.942 ± 0.031

5.703 ± 0.042

2.9 ± 0.023

9.236 ± 0.034

1.943 ± 0.02

4.092 ± 0.027

2.022 ± 0.025

10.518 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.915 ± 0.017

2.103 ± 0.024

5.873 ± 0.031

3.418 ± 0.027

7.697 ± 0.041

5.131 ± 0.024

6.067 ± 0.046

8.401 ± 0.03

1.602 ± 0.017

1.949 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski