Human associated huchismacovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Huchismacovirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5GNX7|A0A0B5GNX7_9VIRU Rep protein OS=Human associated huchismacovirus 2 OX=2169935 GN=rep PE=4 SV=1
MM1 pKa = 7.46TNFVSQKK8 pKa = 9.09FQWFVDD14 pKa = 4.15LQTSSDD20 pKa = 3.41SMQVVTITAGGQRR33 pKa = 11.84VRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84LLPFFAAFKK46 pKa = 9.4YY47 pKa = 10.09YY48 pKa = 10.8KK49 pKa = 9.96LGSVSVRR56 pKa = 11.84FVPASTLPVDD66 pKa = 3.92PTGLSYY72 pKa = 10.98SAGEE76 pKa = 4.1NTVDD80 pKa = 5.06PRR82 pKa = 11.84DD83 pKa = 3.46QFNPGLVRR91 pKa = 11.84ITNGEE96 pKa = 4.11DD97 pKa = 3.45FNIDD101 pKa = 3.29TALLADD107 pKa = 4.25DD108 pKa = 4.15TASQNMYY115 pKa = 8.68YY116 pKa = 10.67QLMLDD121 pKa = 3.58PRR123 pKa = 11.84WYY125 pKa = 10.63KK126 pKa = 10.84FNLQSGFRR134 pKa = 11.84RR135 pKa = 11.84KK136 pKa = 9.91AIPLYY141 pKa = 9.2WSIGQLHH148 pKa = 6.14QDD150 pKa = 3.67VFPGAVQNLPSVTKK164 pKa = 10.46RR165 pKa = 11.84SSGSVNSYY173 pKa = 8.94DD174 pKa = 3.08TGNRR178 pKa = 11.84IDD180 pKa = 4.42HH181 pKa = 6.78IEE183 pKa = 4.2NGSAAFNFYY192 pKa = 10.58SGSNPHH198 pKa = 7.03SIFQVGGKK206 pKa = 9.91DD207 pKa = 3.04RR208 pKa = 11.84MGWMPTDD215 pKa = 3.58AFYY218 pKa = 11.18DD219 pKa = 3.75PGNPDD224 pKa = 3.2LTLGSMAQVPEE235 pKa = 4.19VEE237 pKa = 4.48LLRR240 pKa = 11.84VILPKK245 pKa = 10.27AYY247 pKa = 7.79KK248 pKa = 9.62TKK250 pKa = 10.4FFYY253 pKa = 10.52RR254 pKa = 11.84LYY256 pKa = 10.4IEE258 pKa = 3.94EE259 pKa = 4.25TVNFKK264 pKa = 11.31GPVSMYY270 pKa = 9.15ATNSSVAGSFYY281 pKa = 10.56PLNQVDD287 pKa = 3.78RR288 pKa = 11.84FIYY291 pKa = 9.9PVMNEE296 pKa = 4.33LGSTTQAPARR306 pKa = 11.84NPINGSDD313 pKa = 3.08WHH315 pKa = 6.67NDD317 pKa = 2.62GNAGGEE323 pKa = 4.1NN324 pKa = 3.5

Molecular weight: 36.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5GNX7|A0A0B5GNX7_9VIRU Rep protein OS=Human associated huchismacovirus 2 OX=2169935 GN=rep PE=4 SV=1
MM1 pKa = 8.0PSPKK5 pKa = 9.58WYY7 pKa = 9.89DD8 pKa = 3.07ITVSADD14 pKa = 3.23KK15 pKa = 11.1VPEE18 pKa = 3.86KK19 pKa = 10.7TMVEE23 pKa = 4.13WLNKK27 pKa = 8.11YY28 pKa = 8.88CEE30 pKa = 4.0RR31 pKa = 11.84YY32 pKa = 9.93AYY34 pKa = 10.21GRR36 pKa = 11.84EE37 pKa = 4.0TGEE40 pKa = 3.82NGYY43 pKa = 9.55KK44 pKa = 10.03HH45 pKa = 4.98YY46 pKa = 10.62QIRR49 pKa = 11.84LVLKK53 pKa = 8.29TGADD57 pKa = 3.1ISEE60 pKa = 4.08MRR62 pKa = 11.84KK63 pKa = 8.03VWSAFGHH70 pKa = 5.33VSCTSVRR77 pKa = 11.84NFDD80 pKa = 3.83YY81 pKa = 10.89VLKK84 pKa = 10.68EE85 pKa = 3.62GDD87 pKa = 4.61YY88 pKa = 8.56VCSWIKK94 pKa = 10.52IPEE97 pKa = 4.0NMKK100 pKa = 10.64NPEE103 pKa = 3.63FRR105 pKa = 11.84PWQRR109 pKa = 11.84ALLDD113 pKa = 3.89LEE115 pKa = 4.19QDD117 pKa = 3.5DD118 pKa = 4.25RR119 pKa = 11.84QIDD122 pKa = 4.11VIIDD126 pKa = 3.5LQGNSGKK133 pKa = 10.16SWLTKK138 pKa = 9.84YY139 pKa = 10.33CALKK143 pKa = 9.74MRR145 pKa = 11.84AINVPSGLEE154 pKa = 3.86GKK156 pKa = 10.17DD157 pKa = 3.27IMRR160 pKa = 11.84MCLKK164 pKa = 10.52RR165 pKa = 11.84GAQSGLYY172 pKa = 9.5IFDD175 pKa = 3.76MPRR178 pKa = 11.84AITKK182 pKa = 9.11QSKK185 pKa = 9.26SVWSAIEE192 pKa = 4.23SIKK195 pKa = 10.93NGYY198 pKa = 8.87LWDD201 pKa = 4.71DD202 pKa = 3.86RR203 pKa = 11.84YY204 pKa = 8.77TWEE207 pKa = 4.92EE208 pKa = 3.62KK209 pKa = 10.09WIDD212 pKa = 3.66PPRR215 pKa = 11.84IWVFTNEE222 pKa = 3.93YY223 pKa = 9.29PKK225 pKa = 10.9YY226 pKa = 10.45DD227 pKa = 3.78LVSEE231 pKa = 4.11DD232 pKa = 4.79RR233 pKa = 11.84LRR235 pKa = 11.84FWAPTPTGLVSLAHH249 pKa = 6.38

Molecular weight: 29.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

573

249

324

286.5

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.585 ± 0.472

0.873 ± 0.701

6.457 ± 0.228

4.363 ± 1.273

4.712 ± 1.421

6.981 ± 0.839

1.222 ± 0.01

4.887 ± 0.95

5.41 ± 1.619

7.155 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.967 ± 0.152

5.585 ± 1.216

5.934 ± 0.44

4.014 ± 0.742

5.759 ± 0.411

7.853 ± 0.633

5.236 ± 0.505

6.806 ± 0.483

2.967 ± 1.144

5.236 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski