Bacillus phage AP631

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wbetavirus; unclassified Wbetavirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F2I9|A0A3G8F2I9_9CAUD Uncharacterized protein OS=Bacillus phage AP631 OX=2483609 GN=AP631_0034 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.41KK3 pKa = 10.6FNDD6 pKa = 3.71YY7 pKa = 10.85FGLEE11 pKa = 4.25SKK13 pKa = 10.98SDD15 pKa = 3.8FVWFYY20 pKa = 11.71GFYY23 pKa = 9.19TVSAILIVINMIIVLII39 pKa = 4.21

Molecular weight: 4.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F2J0|A0A3G8F2J0_9CAUD Putative antirepressor OS=Bacillus phage AP631 OX=2483609 GN=AP631_0035 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.3NGKK5 pKa = 9.15RR6 pKa = 11.84LTKK9 pKa = 10.0RR10 pKa = 11.84EE11 pKa = 4.03KK12 pKa = 10.08IHH14 pKa = 6.42LKK16 pKa = 10.36SYY18 pKa = 11.0SLNPDD23 pKa = 2.3NWLVFKK29 pKa = 10.87KK30 pKa = 10.69ADD32 pKa = 3.59GEE34 pKa = 4.2MHH36 pKa = 6.84LVHH39 pKa = 7.45RR40 pKa = 11.84YY41 pKa = 6.0TSRR44 pKa = 11.84TRR46 pKa = 11.84VIPSLL51 pKa = 3.67

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11535

37

1332

206.0

23.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.181 ± 0.619

1.049 ± 0.262

5.635 ± 0.254

8.834 ± 0.28

3.927 ± 0.252

5.817 ± 0.323

1.552 ± 0.203

6.953 ± 0.318

9.788 ± 0.402

8.314 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.159

5.323 ± 0.213

2.557 ± 0.209

4.066 ± 0.322

4.846 ± 0.264

6.025 ± 0.253

5.54 ± 0.357

6.181 ± 0.206

1.136 ± 0.147

3.563 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski