Bacillus phage pW2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R7I5|A0A3Q9R7I5_9CAUD Uncharacterized protein OS=Bacillus phage pW2 OX=2500559 GN=pW2_76 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 5.17KK3 pKa = 11.11EE4 pKa = 4.21NWCYY8 pKa = 11.26NCDD11 pKa = 3.36NHH13 pKa = 7.27FDD15 pKa = 4.24DD16 pKa = 5.4CEE18 pKa = 4.04CWEE21 pKa = 4.48CEE23 pKa = 3.95EE24 pKa = 5.55CGDD27 pKa = 4.19EE28 pKa = 4.42VEE30 pKa = 5.4NGEE33 pKa = 4.89KK34 pKa = 10.39YY35 pKa = 10.21CRR37 pKa = 11.84HH38 pKa = 6.53CKK40 pKa = 9.87PDD42 pKa = 3.96DD43 pKa = 4.06DD44 pKa = 4.91EE45 pKa = 4.36EE46 pKa = 4.28

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IHG2|A0A3T0IHG2_9CAUD Putative guanylate kinase OS=Bacillus phage pW2 OX=2500559 GN=pW2_35 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.56KK3 pKa = 8.9MLKK6 pKa = 10.03GLTVAILMALAIIMAIYY23 pKa = 10.47VGVFLMLGGGLMGIMSVITAGAGMTVLGWSLVKK56 pKa = 10.43ILFAGLAGYY65 pKa = 7.9ATFFVFWLPAMLLLGKK81 pKa = 9.26PKK83 pKa = 10.38KK84 pKa = 10.09RR85 pKa = 11.84FRR87 pKa = 11.84RR88 pKa = 3.74

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

172

0

172

40810

29

3126

237.3

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.577 ± 0.386

0.828 ± 0.094

6.513 ± 0.152

8.27 ± 0.317

4.217 ± 0.157

6.275 ± 0.249

1.894 ± 0.144

6.876 ± 0.185

9.12 ± 0.2

7.866 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.021 ± 0.138

5.883 ± 0.151

2.262 ± 0.108

3.551 ± 0.246

4.097 ± 0.161

5.484 ± 0.213

6.087 ± 0.311

6.618 ± 0.174

1.137 ± 0.077

4.423 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski