Salinivibrio phage SMHB1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Playavirus; Salinivibrio virus SMHB1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D9C9U4|A0A1D9C9U4_9CAUD Putative head completion protein OS=Salinivibrio phage SMHB1 OX=1897436 GN=PP_00031 PE=4 SV=1
MM1 pKa = 7.74SIQDD5 pKa = 3.5NTFAAACYY13 pKa = 9.16DD14 pKa = 3.52QNTIAEE20 pKa = 4.57LEE22 pKa = 4.02ASLLNDD28 pKa = 3.76ADD30 pKa = 4.2AEE32 pKa = 4.52DD33 pKa = 3.74MEE35 pKa = 4.42AWKK38 pKa = 10.35ISEE41 pKa = 4.46SEE43 pKa = 3.64WLEE46 pKa = 4.01QIKK49 pKa = 10.36LALFEE54 pKa = 4.69LYY56 pKa = 10.13KK57 pKa = 11.06DD58 pKa = 3.73EE59 pKa = 4.58FDD61 pKa = 3.63SVEE64 pKa = 4.21FEE66 pKa = 4.01GGEE69 pKa = 4.04YY70 pKa = 10.95ALTQNAHH77 pKa = 6.19ICDD80 pKa = 3.75SGNQYY85 pKa = 9.78QAIAVNEE92 pKa = 3.98NGEE95 pKa = 4.24NFTVLWDD102 pKa = 4.28VISDD106 pKa = 3.93DD107 pKa = 4.6PDD109 pKa = 4.35NEE111 pKa = 4.29TDD113 pKa = 3.64EE114 pKa = 6.38SNMCDD119 pKa = 2.91WEE121 pKa = 4.6KK122 pKa = 10.53PSEE125 pKa = 4.17IVAHH129 pKa = 7.09DD130 pKa = 3.57EE131 pKa = 4.01

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D9CA10|A0A1D9CA10_9CAUD Capsid-scaffolding protein OS=Salinivibrio phage SMHB1 OX=1897436 GN=PP_00028 PE=4 SV=1
MM1 pKa = 7.39TKK3 pKa = 10.23RR4 pKa = 11.84AIKK7 pKa = 10.65GPDD10 pKa = 3.22PAILFFIRR18 pKa = 11.84QRR20 pKa = 11.84IVYY23 pKa = 10.18KK24 pKa = 8.38MTQQDD29 pKa = 3.59VAHH32 pKa = 6.06QAGIPFRR39 pKa = 11.84TYY41 pKa = 10.28QRR43 pKa = 11.84LEE45 pKa = 3.84TGQAEE50 pKa = 4.55LKK52 pKa = 9.6MSQFRR57 pKa = 11.84KK58 pKa = 9.56LCRR61 pKa = 11.84LFDD64 pKa = 3.68VTSVDD69 pKa = 3.4VALGEE74 pKa = 4.04LQFRR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84YY81 pKa = 8.67AADD84 pKa = 3.44VASVAATLPFEE95 pKa = 4.35VRR97 pKa = 11.84EE98 pKa = 4.21HH99 pKa = 6.56FLQLMIAIRR108 pKa = 11.84KK109 pKa = 7.26EE110 pKa = 4.02VV111 pKa = 2.98

Molecular weight: 12.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10178

38

810

207.7

23.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.403 ± 0.549

1.041 ± 0.135

6.259 ± 0.261

6.868 ± 0.371

3.567 ± 0.251

6.445 ± 0.318

2.112 ± 0.215

5.836 ± 0.28

6.111 ± 0.247

8.44 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.24

4.451 ± 0.269

4.058 ± 0.245

4.903 ± 0.264

5.6 ± 0.428

5.816 ± 0.315

5.777 ± 0.242

6.141 ± 0.313

1.533 ± 0.114

2.987 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski