Marine gokushovirus

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5KPB8|U5KPB8_9VIRU Portal protein OS=Marine gokushovirus OX=1385658 PE=4 SV=1
MM1 pKa = 7.49AFVPRR6 pKa = 11.84LQQLPVLNASFIEE19 pKa = 4.29EE20 pKa = 4.42NPPIDD25 pKa = 3.7RR26 pKa = 11.84VVALPTEE33 pKa = 4.36PDD35 pKa = 4.6LLFDD39 pKa = 4.17WYY41 pKa = 11.27FDD43 pKa = 3.88MKK45 pKa = 9.6CTRR48 pKa = 11.84PMPTYY53 pKa = 10.29SVPGLIDD60 pKa = 3.2HH61 pKa = 7.26FF62 pKa = 4.9

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5KQ03|U5KQ03_9VIRU Packaging protein OS=Marine gokushovirus OX=1385658 PE=4 SV=1
MM1 pKa = 6.87MKK3 pKa = 10.02YY4 pKa = 9.59RR5 pKa = 11.84KK6 pKa = 9.73KK7 pKa = 10.22MSAKK11 pKa = 9.92SSRR14 pKa = 11.84KK15 pKa = 9.24QFTKK19 pKa = 10.77GAMKK23 pKa = 10.7VKK25 pKa = 10.15GKK27 pKa = 10.19NFTKK31 pKa = 10.34PMRR34 pKa = 11.84GGIRR38 pKa = 11.84LL39 pKa = 3.45

Molecular weight: 4.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1420

39

473

157.8

17.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.085 ± 1.438

1.549 ± 0.605

5.423 ± 0.814

5.423 ± 1.262

4.718 ± 0.412

8.31 ± 1.071

2.465 ± 0.606

4.718 ± 0.432

6.831 ± 1.418

7.042 ± 0.505

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.958 ± 0.485

4.366 ± 0.467

5.141 ± 0.563

4.648 ± 0.621

4.93 ± 0.538

5.493 ± 0.826

4.718 ± 0.393

6.408 ± 0.744

1.338 ± 0.285

4.437 ± 0.669
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski