French bean severe leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Capulavirus

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7G1P2|J7G1P2_9GEMI Capsid protein OS=French bean severe leaf curl virus OX=1218727 GN=CP PE=3 SV=1
MM1 pKa = 7.78PRR3 pKa = 11.84RR4 pKa = 11.84NNNSFRR10 pKa = 11.84LQGKK14 pKa = 9.58SIFLTYY20 pKa = 9.11PKK22 pKa = 10.58CPLTPLFVIDD32 pKa = 4.87YY33 pKa = 9.9LYY35 pKa = 11.05QLLKK39 pKa = 10.77NFNPIYY45 pKa = 10.86ARR47 pKa = 11.84VCTEE51 pKa = 3.42NHH53 pKa = 6.07QDD55 pKa = 3.87GEE57 pKa = 4.4PHH59 pKa = 6.43LHH61 pKa = 6.94CLVQLDD67 pKa = 3.87KK68 pKa = 11.31RR69 pKa = 11.84FNTTSQRR76 pKa = 11.84YY77 pKa = 8.69FDD79 pKa = 3.87ISDD82 pKa = 3.71PNRR85 pKa = 11.84TGVYY89 pKa = 9.84HH90 pKa = 6.69PNCQVPRR97 pKa = 11.84RR98 pKa = 11.84DD99 pKa = 3.84ADD101 pKa = 3.58VADD104 pKa = 4.81YY105 pKa = 10.27IAKK108 pKa = 10.16GGQFEE113 pKa = 4.22EE114 pKa = 4.62RR115 pKa = 11.84GILRR119 pKa = 11.84ASRR122 pKa = 11.84RR123 pKa = 11.84SPKK126 pKa = 9.94KK127 pKa = 10.16SRR129 pKa = 11.84DD130 pKa = 3.99SIWTNIINEE139 pKa = 4.36STSKK143 pKa = 10.98SEE145 pKa = 3.65FLGRR149 pKa = 11.84VQIEE153 pKa = 4.17QPYY156 pKa = 9.38VWATQLRR163 pKa = 11.84NLEE166 pKa = 4.0YY167 pKa = 10.4AANSKK172 pKa = 9.6WPEE175 pKa = 3.88QPSVYY180 pKa = 9.68IPKK183 pKa = 8.49WTVFNNVPEE192 pKa = 5.28PIRR195 pKa = 11.84EE196 pKa = 4.03WADD199 pKa = 3.31TNLFTVSLQSMQLVQPEE216 pKa = 3.99ISVTDD221 pKa = 3.68MQWAHH226 pKa = 6.35NLTEE230 pKa = 5.42DD231 pKa = 5.21FITDD235 pKa = 3.15EE236 pKa = 4.47WIGNSEE242 pKa = 4.27DD243 pKa = 3.75PVVQSFSGSEE253 pKa = 3.82TGPPSDD259 pKa = 5.17SNNCLAQQKK268 pKa = 7.36QAKK271 pKa = 7.39QHH273 pKa = 6.35GPGAWVYY280 pKa = 10.28IIIFVGVLILVCGIILLLTPSS301 pKa = 3.43

Molecular weight: 34.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7G1P2|J7G1P2_9GEMI Capsid protein OS=French bean severe leaf curl virus OX=1218727 GN=CP PE=3 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84TRR5 pKa = 11.84SGRR8 pKa = 11.84QYY10 pKa = 10.92SAQAPSWGRR19 pKa = 11.84KK20 pKa = 5.17KK21 pKa = 9.17TRR23 pKa = 11.84TPRR26 pKa = 11.84SRR28 pKa = 11.84PTLIGPIRR36 pKa = 11.84RR37 pKa = 11.84PNYY40 pKa = 9.4QIKK43 pKa = 8.5TRR45 pKa = 11.84YY46 pKa = 9.14APHH49 pKa = 6.95RR50 pKa = 11.84PQTKK54 pKa = 8.93IHH56 pKa = 6.21SLSAARR62 pKa = 11.84VVSGSDD68 pKa = 2.89NGYY71 pKa = 8.75GWHH74 pKa = 6.87ISDD77 pKa = 4.11VPIGSGFEE85 pKa = 4.22DD86 pKa = 3.08RR87 pKa = 11.84HH88 pKa = 6.35SDD90 pKa = 4.03KK91 pKa = 10.91IRR93 pKa = 11.84ILNFNFKK100 pKa = 10.56LQMRR104 pKa = 11.84TSQQGQNTSCWHH116 pKa = 5.12NVYY119 pKa = 10.72LFLVRR124 pKa = 11.84DD125 pKa = 3.94NSGGTAVPKK134 pKa = 10.51FNSICMMDD142 pKa = 3.69NSNPSTAEE150 pKa = 3.47IDD152 pKa = 3.61HH153 pKa = 6.87DD154 pKa = 4.33SKK156 pKa = 11.82DD157 pKa = 2.99RR158 pKa = 11.84FTIMRR163 pKa = 11.84RR164 pKa = 11.84WRR166 pKa = 11.84FQFKK170 pKa = 10.73GNSTSGSVAYY180 pKa = 10.25DD181 pKa = 3.62CARR184 pKa = 11.84NTYY187 pKa = 9.93DD188 pKa = 2.84FRR190 pKa = 11.84KK191 pKa = 9.1FVKK194 pKa = 10.27LSSITEE200 pKa = 4.36FKK202 pKa = 10.83SATSGSYY209 pKa = 11.56ANTQKK214 pKa = 10.86NAWVLYY220 pKa = 7.21FVPQTYY226 pKa = 11.35DD227 pKa = 3.06MTVDD231 pKa = 3.31GHH233 pKa = 5.43CTIKK237 pKa = 10.77YY238 pKa = 9.39VSIVV242 pKa = 3.08

Molecular weight: 27.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

543

242

301

271.5

31.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.788 ± 0.382

1.842 ± 0.123

5.157 ± 0.141

3.499 ± 1.478

4.604 ± 0.232

5.341 ± 0.561

2.394 ± 0.326

6.446 ± 0.432

4.788 ± 0.652

5.893 ± 1.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.537

6.262 ± 0.312

6.077 ± 1.002

5.709 ± 0.761

7.182 ± 1.248

9.208 ± 1.274

6.63 ± 0.799

6.077 ± 0.461

2.394 ± 0.214

4.052 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski