Marine snail associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL52|A0A0K1RL52_9CIRC Putative replication initiation protein OS=Marine snail associated circular virus OX=1692255 PE=4 SV=1
MM1 pKa = 7.71AHH3 pKa = 6.5RR4 pKa = 11.84QHH6 pKa = 6.86AAKK9 pKa = 10.11RR10 pKa = 11.84WVFTINNWTAAEE22 pKa = 3.96QQALIDD28 pKa = 3.79SSDD31 pKa = 3.44NFDD34 pKa = 3.69YY35 pKa = 11.36LCFGRR40 pKa = 11.84EE41 pKa = 3.63RR42 pKa = 11.84GDD44 pKa = 3.23NNTPHH49 pKa = 6.27LQGYY53 pKa = 9.13VILKK57 pKa = 8.72TKK59 pKa = 10.62LRR61 pKa = 11.84LNNVKK66 pKa = 10.15ALPGFRR72 pKa = 11.84RR73 pKa = 11.84CHH75 pKa = 6.9LEE77 pKa = 3.72VSRR80 pKa = 11.84GTPQQAADD88 pKa = 3.74YY89 pKa = 9.05CKK91 pKa = 10.44KK92 pKa = 10.81DD93 pKa = 2.99GDD95 pKa = 3.88FEE97 pKa = 4.73EE98 pKa = 5.61FGEE101 pKa = 4.35LPKK104 pKa = 11.19GQGKK108 pKa = 10.23RR109 pKa = 11.84SDD111 pKa = 3.71FEE113 pKa = 4.4NLKK116 pKa = 10.35EE117 pKa = 4.2WIKK120 pKa = 11.26SLDD123 pKa = 3.38HH124 pKa = 6.72WPDD127 pKa = 2.98DD128 pKa = 3.66HH129 pKa = 7.23EE130 pKa = 4.42IAEE133 pKa = 4.91EE134 pKa = 4.46YY135 pKa = 9.61PSLWGRR141 pKa = 11.84YY142 pKa = 7.85RR143 pKa = 11.84SACEE147 pKa = 3.67SFRR150 pKa = 11.84QLFGKK155 pKa = 9.32PIEE158 pKa = 4.5VVDD161 pKa = 3.78PTTFEE166 pKa = 3.86PRR168 pKa = 11.84VWQQRR173 pKa = 11.84IIDD176 pKa = 3.96ICNGEE181 pKa = 3.93PDD183 pKa = 3.42PRR185 pKa = 11.84KK186 pKa = 10.01VYY188 pKa = 10.24FVVDD192 pKa = 3.75EE193 pKa = 4.58NGNTGKK199 pKa = 10.54SYY201 pKa = 11.16LSAYY205 pKa = 9.81LISKK209 pKa = 10.1FPDD212 pKa = 2.96EE213 pKa = 4.22VQILSVGRR221 pKa = 11.84RR222 pKa = 11.84DD223 pKa = 4.49DD224 pKa = 3.66LAHH227 pKa = 7.35AINPRR232 pKa = 11.84RR233 pKa = 11.84SIFLFDD239 pKa = 3.6VPRR242 pKa = 11.84GGMEE246 pKa = 3.76YY247 pKa = 10.24LQYY250 pKa = 10.96TIFEE254 pKa = 4.16QLKK257 pKa = 8.49NRR259 pKa = 11.84TVFSPKK265 pKa = 9.81YY266 pKa = 10.25NSITKK271 pKa = 10.05ILRR274 pKa = 11.84KK275 pKa = 9.29LPHH278 pKa = 6.31VIIFSNEE285 pKa = 3.77SPDD288 pKa = 3.81RR289 pKa = 11.84NKK291 pKa = 10.0MSHH294 pKa = 6.57DD295 pKa = 3.97RR296 pKa = 11.84YY297 pKa = 9.97HH298 pKa = 5.43VTHH301 pKa = 6.79IRR303 pKa = 11.84QFPRR307 pKa = 11.84ADD309 pKa = 3.4DD310 pKa = 3.81NGANGG315 pKa = 3.47

Molecular weight: 36.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL52|A0A0K1RL52_9CIRC Putative replication initiation protein OS=Marine snail associated circular virus OX=1692255 PE=4 SV=1
MM1 pKa = 7.73SFEE4 pKa = 4.25TAVTAGFVAGYY15 pKa = 9.98GYY17 pKa = 10.0VARR20 pKa = 11.84EE21 pKa = 4.11TYY23 pKa = 10.12RR24 pKa = 11.84ALQEE28 pKa = 4.2GRR30 pKa = 11.84SQSMYY35 pKa = 10.34HH36 pKa = 5.17YY37 pKa = 10.27NLRR40 pKa = 11.84KK41 pKa = 9.6RR42 pKa = 11.84KK43 pKa = 8.06PSAVITPSKK52 pKa = 10.62APVIRR57 pKa = 11.84RR58 pKa = 11.84TAPTQAPEE66 pKa = 3.94MRR68 pKa = 11.84GLTVHH73 pKa = 6.39TRR75 pKa = 11.84TTRR78 pKa = 11.84PWRR81 pKa = 11.84TSNYY85 pKa = 6.86RR86 pKa = 11.84TLGRR90 pKa = 11.84RR91 pKa = 11.84PGKK94 pKa = 9.44YY95 pKa = 9.58GSRR98 pKa = 11.84KK99 pKa = 8.17RR100 pKa = 11.84TISADD105 pKa = 3.42NTSSAEE111 pKa = 3.98KK112 pKa = 9.73MPSDD116 pKa = 3.94KK117 pKa = 10.91SVSADD122 pKa = 2.95GLIFVEE128 pKa = 4.55QQSTTSQINKK138 pKa = 9.24RR139 pKa = 11.84RR140 pKa = 11.84GEE142 pKa = 4.0LVNIRR147 pKa = 11.84GVKK150 pKa = 9.22VRR152 pKa = 11.84YY153 pKa = 8.17FFKK156 pKa = 10.37MNQRR160 pKa = 11.84LNPTNAITIPVRR172 pKa = 11.84VRR174 pKa = 11.84WAIIIPKK181 pKa = 10.01ADD183 pKa = 3.67TLTLTTVGTAPNSYY197 pKa = 10.39SRR199 pKa = 11.84PALPNFFINEE209 pKa = 3.99NPVDD213 pKa = 4.07RR214 pKa = 11.84VAKK217 pKa = 10.67DD218 pKa = 3.31FDD220 pKa = 3.99TSVPNPGNHH229 pKa = 4.88WDD231 pKa = 3.48MFNRR235 pKa = 11.84KK236 pKa = 8.31INRR239 pKa = 11.84EE240 pKa = 3.94EE241 pKa = 3.97YY242 pKa = 10.28SVLKK246 pKa = 10.15EE247 pKa = 4.02GQFILCQDD255 pKa = 3.93GGNGEE260 pKa = 4.3LSGIRR265 pKa = 11.84PRR267 pKa = 11.84PMRR270 pKa = 11.84TYY272 pKa = 10.6KK273 pKa = 10.47HH274 pKa = 6.31LNFYY278 pKa = 10.59IPIKK282 pKa = 9.98RR283 pKa = 11.84QMHH286 pKa = 4.84FTGTGGEE293 pKa = 4.1PDD295 pKa = 3.49ANVYY299 pKa = 8.34MLYY302 pKa = 9.68WYY304 pKa = 10.43AFEE307 pKa = 4.99GSNAATKK314 pKa = 10.41DD315 pKa = 3.1IADD318 pKa = 3.97NADD321 pKa = 3.31KK322 pKa = 10.91PFVEE326 pKa = 5.02FHH328 pKa = 6.7EE329 pKa = 4.43KK330 pKa = 7.34TTYY333 pKa = 8.21FTNSDD338 pKa = 3.42MFKK341 pKa = 10.98

Molecular weight: 38.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

656

315

341

328.0

37.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.098 ± 0.739

0.915 ± 0.488

5.64 ± 1.206

5.488 ± 0.625

5.335 ± 0.275

6.25 ± 0.158

2.591 ± 0.654

5.64 ± 0.284

5.945 ± 0.063

5.64 ± 0.976

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.747

6.098 ± 0.278

6.098 ± 0.278

3.659 ± 0.571

8.689 ± 0.316

6.098 ± 0.278

6.555 ± 1.993

5.488 ± 0.297

1.524 ± 0.276

4.268 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski