Hudisavirus sp.

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223Q5V2|A0A223Q5V2_9VIRU Putative capsid protein OS=Hudisavirus sp. OX=2021738 PE=4 SV=1
MM1 pKa = 7.59IDD3 pKa = 3.06SMAAEE8 pKa = 4.28TAAKK12 pKa = 9.75RR13 pKa = 11.84WCFTINNPTDD23 pKa = 3.38EE24 pKa = 4.5DD25 pKa = 4.29KK26 pKa = 10.99FWEE29 pKa = 4.16NAEE32 pKa = 4.0QQEE35 pKa = 4.1QLEE38 pKa = 4.27YY39 pKa = 10.87LIVQHH44 pKa = 6.33EE45 pKa = 4.58VGEE48 pKa = 4.32QGTPHH53 pKa = 5.68YY54 pKa = 10.51QGFLILKK61 pKa = 9.3RR62 pKa = 11.84KK63 pKa = 9.22NRR65 pKa = 11.84LSWLKK70 pKa = 10.97KK71 pKa = 9.19NINSRR76 pKa = 11.84AHH78 pKa = 5.34WEE80 pKa = 4.11KK81 pKa = 10.26TRR83 pKa = 11.84GTDD86 pKa = 3.35QQAADD91 pKa = 4.0YY92 pKa = 10.33CRR94 pKa = 11.84KK95 pKa = 10.26DD96 pKa = 3.38DD97 pKa = 3.67THH99 pKa = 7.69PEE101 pKa = 3.64GSLRR105 pKa = 11.84FEE107 pKa = 3.93WGTLKK112 pKa = 9.92TALKK116 pKa = 10.53RR117 pKa = 11.84KK118 pKa = 10.06DD119 pKa = 3.57RR120 pKa = 11.84DD121 pKa = 3.77CLEE124 pKa = 3.95EE125 pKa = 4.02AVIEE129 pKa = 4.16EE130 pKa = 4.24VKK132 pKa = 10.58KK133 pKa = 10.91IQDD136 pKa = 3.11EE137 pKa = 4.61GFRR140 pKa = 11.84SVRR143 pKa = 11.84DD144 pKa = 3.14IDD146 pKa = 3.95PQVLARR152 pKa = 11.84PGFMAAYY159 pKa = 8.95NALTADD165 pKa = 3.53MLGVYY170 pKa = 9.74RR171 pKa = 11.84PEE173 pKa = 3.99LKK175 pKa = 9.86IVTMVGPPGTGKK187 pKa = 10.42SYY189 pKa = 10.9AINTLFPHH197 pKa = 7.24AGRR200 pKa = 11.84AIMGNGGTWFANPTATVMVFEE221 pKa = 4.84EE222 pKa = 4.5FAGQIPLQKK231 pKa = 9.48MLKK234 pKa = 10.37LLDD237 pKa = 4.66PYY239 pKa = 11.18PMALEE244 pKa = 4.52VKK246 pKa = 10.15GGMRR250 pKa = 11.84PAMYY254 pKa = 9.13TLVIITSNTRR264 pKa = 11.84PDD266 pKa = 2.75GWYY269 pKa = 10.16KK270 pKa = 10.8EE271 pKa = 4.2EE272 pKa = 4.01EE273 pKa = 4.14QGGKK277 pKa = 8.16RR278 pKa = 11.84TDD280 pKa = 3.27ALLALWDD287 pKa = 3.9RR288 pKa = 11.84LGFSNGTNRR297 pKa = 11.84CYY299 pKa = 9.72RR300 pKa = 11.84TCGTYY305 pKa = 10.46LEE307 pKa = 4.79PVRR310 pKa = 11.84GMALGAAGTWIDD322 pKa = 3.61NTRR325 pKa = 11.84TWFMNEE331 pKa = 3.48LAKK334 pKa = 10.47AAHH337 pKa = 5.57VEE339 pKa = 4.09EE340 pKa = 5.71HH341 pKa = 6.84EE342 pKa = 4.38EE343 pKa = 4.07LSDD346 pKa = 3.54EE347 pKa = 4.47ALSQVEE353 pKa = 3.96QDD355 pKa = 3.96KK356 pKa = 11.74LEE358 pKa = 4.57DD359 pKa = 5.55DD360 pKa = 3.78IASLDD365 pKa = 3.59VV366 pKa = 3.54

Molecular weight: 41.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223Q5V2|A0A223Q5V2_9VIRU Putative capsid protein OS=Hudisavirus sp. OX=2021738 PE=4 SV=1
MM1 pKa = 6.98PTYY4 pKa = 9.82VYY6 pKa = 10.43KK7 pKa = 10.49YY8 pKa = 7.97RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 7.5GRR14 pKa = 11.84RR15 pKa = 11.84YY16 pKa = 8.92SRR18 pKa = 11.84YY19 pKa = 8.02RR20 pKa = 11.84RR21 pKa = 11.84YY22 pKa = 9.69SGAYY26 pKa = 7.57SRR28 pKa = 11.84YY29 pKa = 7.74RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84SGTSSTASSRR43 pKa = 11.84GRR45 pKa = 11.84IRR47 pKa = 11.84VRR49 pKa = 11.84VPIQQVVTMTVPAGSIDD66 pKa = 4.21SNLLTSSPFYY76 pKa = 7.79WTSSITSAPYY86 pKa = 9.63GRR88 pKa = 11.84CGAVATPLYY97 pKa = 10.09RR98 pKa = 11.84AYY100 pKa = 9.82TSLYY104 pKa = 10.57DD105 pKa = 3.56QVKK108 pKa = 10.25CDD110 pKa = 3.55GVVTNLSVVSPIGGGSGAAASALQVIVAYY139 pKa = 10.23DD140 pKa = 3.32RR141 pKa = 11.84QGNFNEE147 pKa = 4.17VLNGDD152 pKa = 4.3GVTVAQLFNFSSAVVRR168 pKa = 11.84SAINNSVAKK177 pKa = 9.78MSRR180 pKa = 11.84SCWASDD186 pKa = 3.31LQEE189 pKa = 3.39RR190 pKa = 11.84TMFHH194 pKa = 7.39DD195 pKa = 4.18CTVSASGNPVNTITDD210 pKa = 3.46GDD212 pKa = 4.02FSSNQQKK219 pKa = 10.31VGYY222 pKa = 7.91FAPLTYY228 pKa = 10.62VGLRR232 pKa = 11.84LAAAAPTSDD241 pKa = 5.37LSIQVLLEE249 pKa = 3.47QTYY252 pKa = 9.51YY253 pKa = 9.61FTFRR257 pKa = 11.84NPKK260 pKa = 10.07FGADD264 pKa = 3.51TSNSASFSVRR274 pKa = 11.84PVTMDD279 pKa = 3.17SAVDD283 pKa = 3.54TRR285 pKa = 11.84TMQSTQVMDD294 pKa = 4.75DD295 pKa = 4.3DD296 pKa = 5.79GGLDD300 pKa = 4.49DD301 pKa = 5.34EE302 pKa = 5.22AAQAAQPAARR312 pKa = 11.84VARR315 pKa = 11.84ATPLSSLFDD324 pKa = 3.59SSIPVPARR332 pKa = 11.84FRR334 pKa = 11.84QQ335 pKa = 3.37

Molecular weight: 36.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

701

335

366

350.5

39.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.7 ± 0.512

1.284 ± 0.062

5.991 ± 0.219

5.136 ± 2.698

3.566 ± 0.215

7.133 ± 0.387

1.141 ± 0.577

3.709 ± 0.496

3.852 ± 1.615

7.133 ± 1.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.523

3.994 ± 0.078

4.565 ± 0.145

4.422 ± 0.242

7.561 ± 1.159

7.703 ± 3.513

7.418 ± 0.235

6.847 ± 1.647

1.712 ± 0.763

4.28 ± 0.953
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski