Roseobacter phage CRP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QW36|A0A646QW36_9CAUD Uncharacterized protein OS=Roseobacter phage CRP-1 OX=2559280 GN=CRP1_gp18 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.44IMGYY6 pKa = 9.85EE7 pKa = 3.71IVVEE11 pKa = 3.91IDD13 pKa = 3.19GVEE16 pKa = 4.2SVIQLDD22 pKa = 3.72DD23 pKa = 3.85TYY25 pKa = 11.36PAINDD30 pKa = 3.03WHH32 pKa = 6.3TATEE36 pKa = 4.17FAMRR40 pKa = 11.84LAQHH44 pKa = 6.35EE45 pKa = 4.68HH46 pKa = 7.08PDD48 pKa = 3.26ANQIDD53 pKa = 4.23FVEE56 pKa = 4.54CGEE59 pKa = 4.42YY60 pKa = 10.36EE61 pKa = 4.06LEE63 pKa = 4.37EE64 pKa = 4.02YY65 pKa = 9.98KK66 pKa = 10.8QYY68 pKa = 11.67DD69 pKa = 4.59FIHH72 pKa = 6.58EE73 pKa = 4.47APFMIQQ79 pKa = 2.86

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QVI9|A0A646QVI9_9CAUD Putative tail structural protein OS=Roseobacter phage CRP-1 OX=2559280 GN=CRP1_gp37 PE=4 SV=1
MM1 pKa = 7.29YY2 pKa = 10.54NVGFQTMNNLVLPRR16 pKa = 11.84EE17 pKa = 4.29EE18 pKa = 4.18KK19 pKa = 9.0TQQEE23 pKa = 4.44TSMGLLARR31 pKa = 11.84NMPSKK36 pKa = 10.55PKK38 pKa = 10.04TGEE41 pKa = 3.61QQVRR45 pKa = 11.84QRR47 pKa = 11.84VANYY51 pKa = 9.31VSQIRR56 pKa = 11.84KK57 pKa = 9.52ARR59 pKa = 11.84MEE61 pKa = 4.3LKK63 pKa = 10.74NGG65 pKa = 3.48

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

16856

42

1251

251.6

27.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.362 ± 0.448

0.629 ± 0.161

6.425 ± 0.183

6.597 ± 0.569

3.548 ± 0.186

7.517 ± 0.432

1.56 ± 0.205

4.758 ± 0.167

5.707 ± 0.558

7.042 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.196

5.458 ± 0.334

3.672 ± 0.238

4.521 ± 0.551

4.817 ± 0.372

6.467 ± 0.339

7.528 ± 0.67

6.395 ± 0.21

1.287 ± 0.147

3.66 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski