Actinophytocola sp.

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinophytocola; unclassified Actinophytocola

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4585 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L5ESG1|A0A6L5ESG1_9PSEU Uncharacterized protein OS=Actinophytocola sp. OX=1872138 GN=GEV00_06790 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 10.03FGVMLAACAMAGASVVSTQTATAEE28 pKa = 4.36TLGSEE33 pKa = 4.18LGSYY37 pKa = 9.88IVVLEE42 pKa = 4.79DD43 pKa = 3.56GVDD46 pKa = 3.45AASVAQQQVGALGGIVGHH64 pKa = 6.33VYY66 pKa = 10.72GSALSGYY73 pKa = 9.06SAQLTDD79 pKa = 3.42AARR82 pKa = 11.84DD83 pKa = 3.59RR84 pKa = 11.84LATYY88 pKa = 8.71PAVDD92 pKa = 4.57YY93 pKa = 10.62IQADD97 pKa = 3.98TPVRR101 pKa = 11.84ATQTMPTGIDD111 pKa = 3.06RR112 pKa = 11.84TDD114 pKa = 3.62AEE116 pKa = 4.4QSPTAAIDD124 pKa = 4.07GSDD127 pKa = 3.3EE128 pKa = 4.16RR129 pKa = 11.84VDD131 pKa = 3.29VDD133 pKa = 3.96VAVIDD138 pKa = 3.81TGADD142 pKa = 3.16MDD144 pKa = 4.61HH145 pKa = 7.6PDD147 pKa = 3.48LNVYY151 pKa = 10.05EE152 pKa = 4.91EE153 pKa = 4.43GAKK156 pKa = 10.25NCSTLSLSPEE166 pKa = 4.28DD167 pKa = 4.02EE168 pKa = 4.56NGHH171 pKa = 4.93GTHH174 pKa = 6.1VAGTIGALDD183 pKa = 4.13DD184 pKa = 4.05ASGVVGMAPGARR196 pKa = 11.84IWPVKK201 pKa = 10.08VLNALGMGLTSDD213 pKa = 4.38VICGIDD219 pKa = 3.47YY220 pKa = 10.77VAAHH224 pKa = 6.46SDD226 pKa = 3.83EE227 pKa = 5.01IEE229 pKa = 4.17VANMSLGGSGSDD241 pKa = 4.93DD242 pKa = 3.86GNCGEE247 pKa = 5.35DD248 pKa = 3.87NNDD251 pKa = 3.35AQHH254 pKa = 6.92AAICNAVDD262 pKa = 3.25AGVTFVVAAGNDD274 pKa = 3.51SADD277 pKa = 3.45ARR279 pKa = 11.84EE280 pKa = 4.4TVPAAYY286 pKa = 10.17DD287 pKa = 3.48EE288 pKa = 4.97VITVSALADD297 pKa = 3.69FDD299 pKa = 4.08GQPGGRR305 pKa = 11.84AAPTCRR311 pKa = 11.84DD312 pKa = 3.7DD313 pKa = 5.24VDD315 pKa = 3.87DD316 pKa = 4.32TFADD320 pKa = 4.97FSNYY324 pKa = 10.09GDD326 pKa = 5.33DD327 pKa = 4.04IDD329 pKa = 5.06VIAPGVCIEE338 pKa = 4.16STSLGGGYY346 pKa = 7.61EE347 pKa = 4.28TLSGTSMASPHH358 pKa = 6.26AAGAAALYY366 pKa = 9.07QANNPAASPEE376 pKa = 4.12QVKK379 pKa = 10.21RR380 pKa = 11.84ALQNAGTTDD389 pKa = 2.91WTFPSEE395 pKa = 4.2DD396 pKa = 3.36RR397 pKa = 11.84DD398 pKa = 4.4GIQEE402 pKa = 3.98MLVNVAGFF410 pKa = 3.38

Molecular weight: 41.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L5ERD4|A0A6L5ERD4_9PSEU YCII domain-containing protein OS=Actinophytocola sp. OX=1872138 GN=GEV00_04855 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84KK18 pKa = 8.57HH19 pKa = 4.77GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.74GRR42 pKa = 11.84AKK44 pKa = 10.68LSAA47 pKa = 3.92

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4585

0

4585

1350462

17

5027

294.5

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.078 ± 0.045

0.829 ± 0.01

6.396 ± 0.032

5.844 ± 0.036

2.77 ± 0.021

9.081 ± 0.033

2.316 ± 0.018

3.749 ± 0.025

2.105 ± 0.023

10.177 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.862 ± 0.013

1.884 ± 0.019

5.648 ± 0.027

2.818 ± 0.023

7.98 ± 0.04

5.119 ± 0.021

6.052 ± 0.025

8.8 ± 0.034

1.441 ± 0.016

2.049 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski