Lactobacillus phage phiPYB5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2KRC4|D2KRC4_9CAUD BppU_N domain-containing protein OS=Lactobacillus phage phiPYB5 OX=438780 GN=CU5_20 PE=4 SV=1
MM1 pKa = 7.34FNLFGSLEE9 pKa = 4.13MYY11 pKa = 10.68LNNLIKK17 pKa = 10.5VANSYY22 pKa = 10.62GVLDD26 pKa = 3.92QQTGQATTTVILTPPTGLTLDD47 pKa = 3.89SDD49 pKa = 4.19FVDD52 pKa = 5.71AINALWSTIEE62 pKa = 3.92STLNNLQSYY71 pKa = 10.83ANEE74 pKa = 4.1FF75 pKa = 3.5

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2KRD3|D2KRD3_9CAUD Uncharacterized protein OS=Lactobacillus phage phiPYB5 OX=438780 GN=CU5_31 PE=4 SV=1
MM1 pKa = 7.96LEE3 pKa = 3.84VCMKK7 pKa = 8.73IAVYY11 pKa = 10.6VRR13 pKa = 11.84VSTLEE18 pKa = 3.83QAEE21 pKa = 4.25SGYY24 pKa = 10.92SIGEE28 pKa = 3.89QTEE31 pKa = 4.12KK32 pKa = 10.08LTAYY36 pKa = 10.28CRR38 pKa = 11.84VKK40 pKa = 10.25DD41 pKa = 3.54WQVAKK46 pKa = 10.39IYY48 pKa = 9.33TDD50 pKa = 3.52PGFSGSSLDD59 pKa = 3.75RR60 pKa = 11.84PAMQQLIGDD69 pKa = 4.39CKK71 pKa = 10.83LGVFDD76 pKa = 5.52AVLVYY81 pKa = 10.81KK82 pKa = 10.59LDD84 pKa = 3.8RR85 pKa = 11.84LSRR88 pKa = 11.84SQKK91 pKa = 8.91DD92 pKa = 3.35TLYY95 pKa = 11.05LIEE98 pKa = 5.4DD99 pKa = 3.6VFNAYY104 pKa = 10.12NIHH107 pKa = 6.31FMSLSEE113 pKa = 4.46NFDD116 pKa = 3.17TSTPFGKK123 pKa = 10.57AMIGLLSVFAQLEE136 pKa = 4.12RR137 pKa = 11.84EE138 pKa = 4.22QIKK141 pKa = 10.5EE142 pKa = 3.7RR143 pKa = 11.84MQMGKK148 pKa = 10.18LGRR151 pKa = 11.84VKK153 pKa = 10.42AGKK156 pKa = 9.81ISAWSNVPFGYY167 pKa = 9.3TKK169 pKa = 10.82VKK171 pKa = 10.57DD172 pKa = 4.02SYY174 pKa = 11.86DD175 pKa = 3.79VDD177 pKa = 3.89PLRR180 pKa = 11.84ADD182 pKa = 3.3IVRR185 pKa = 11.84RR186 pKa = 11.84IYY188 pKa = 11.28ADD190 pKa = 3.39YY191 pKa = 10.85LAGKK195 pKa = 9.92SITKK199 pKa = 9.68IMKK202 pKa = 8.03EE203 pKa = 4.02LNRR206 pKa = 11.84EE207 pKa = 3.74GHH209 pKa = 5.88IGKK212 pKa = 9.82GVAWSYY218 pKa = 9.08RR219 pKa = 11.84TVRR222 pKa = 11.84QVLGNVTYY230 pKa = 9.8TGRR233 pKa = 11.84IIFKK237 pKa = 9.61GQVYY241 pKa = 10.45DD242 pKa = 4.04GLHH245 pKa = 5.7TGIISKK251 pKa = 10.64ADD253 pKa = 3.07WDD255 pKa = 3.9EE256 pKa = 3.97VQRR259 pKa = 11.84VLKK262 pKa = 10.31IRR264 pKa = 11.84QLDD267 pKa = 3.57QARR270 pKa = 11.84KK271 pKa = 9.41SNNPRR276 pKa = 11.84PFQARR281 pKa = 11.84YY282 pKa = 8.11MLSGLLKK289 pKa = 10.28CHH291 pKa = 6.0YY292 pKa = 10.11CGATLAIAKK301 pKa = 9.33SHH303 pKa = 5.75TKK305 pKa = 10.38NGPLWRR311 pKa = 11.84YY312 pKa = 7.74VCPTRR317 pKa = 11.84HH318 pKa = 5.33ANKK321 pKa = 9.54YY322 pKa = 8.65HH323 pKa = 6.75RR324 pKa = 11.84NNAARR329 pKa = 11.84YY330 pKa = 7.68RR331 pKa = 11.84IPPVNCEE338 pKa = 3.59LTYY341 pKa = 10.38KK342 pKa = 10.9YY343 pKa = 10.72KK344 pKa = 10.67DD345 pKa = 3.74DD346 pKa = 4.45LEE348 pKa = 4.32EE349 pKa = 4.17AVLRR353 pKa = 11.84EE354 pKa = 4.15VKK356 pKa = 10.26KK357 pKa = 10.98VALDD361 pKa = 3.47PDD363 pKa = 4.08AVVASQDD370 pKa = 3.57DD371 pKa = 4.27GQPKK375 pKa = 9.41IDD377 pKa = 3.62KK378 pKa = 10.3QAIKK382 pKa = 10.6AQLAKK387 pKa = 10.15IKK389 pKa = 10.12RR390 pKa = 11.84QQAKK394 pKa = 10.31LVDD397 pKa = 5.09LYY399 pKa = 11.6LLSDD403 pKa = 4.25DD404 pKa = 5.8LMLTSFTIGPKK415 pKa = 9.1SLKK418 pKa = 10.12SKK420 pKa = 10.6LMLSRR425 pKa = 11.84HH426 pKa = 5.8NLSMMPKK433 pKa = 9.89RR434 pKa = 11.84PTPLKK439 pKa = 9.78RR440 pKa = 11.84RR441 pKa = 11.84RR442 pKa = 11.84PMLHH446 pKa = 6.67RR447 pKa = 11.84LISS450 pKa = 3.52

Molecular weight: 51.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10059

36

966

218.7

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.705 ± 0.453

0.557 ± 0.098

7.188 ± 0.374

5.1 ± 0.446

3.539 ± 0.194

7.287 ± 0.416

1.74 ± 0.163

6.541 ± 0.245

7.058 ± 0.433

7.864 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.535 ± 0.127

5.766 ± 0.224

3.519 ± 0.192

4.265 ± 0.271

4.016 ± 0.3

6.601 ± 0.387

6.83 ± 0.442

6.263 ± 0.242

1.66 ± 0.152

3.967 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski