Leptothrix ochracea L12

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Leptothrix; Leptothrix ochracea

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4Z631|I4Z631_9BURK GDT1 family protein OS=Leptothrix ochracea L12 OX=735332 GN=LepocDRAFT_00004070 PE=3 SV=1
MM1 pKa = 7.44NEE3 pKa = 3.95STVNPSVMPAIGPTQQGAGVNHH25 pKa = 7.43GGLSITPQRR34 pKa = 11.84HH35 pKa = 5.26EE36 pKa = 4.24GHH38 pKa = 6.37PTAAVTPAPLAHH50 pKa = 7.34RR51 pKa = 11.84FGQAPDD57 pKa = 3.68TEE59 pKa = 4.6GTEE62 pKa = 4.22EE63 pKa = 5.61DD64 pKa = 4.4SIATNAVEE72 pKa = 4.24TVEE75 pKa = 4.43TLDD78 pKa = 3.87EE79 pKa = 4.3DD80 pKa = 4.25TFGADD85 pKa = 3.06ISDD88 pKa = 3.63GTEE91 pKa = 3.75AFDD94 pKa = 4.5PGTMGHH100 pKa = 6.19EE101 pKa = 4.09SSGRR105 pKa = 11.84EE106 pKa = 3.56DD107 pKa = 3.48TLRR110 pKa = 11.84YY111 pKa = 9.37SALHH115 pKa = 6.35EE116 pKa = 4.2PAMMLTAADD125 pKa = 3.67ATFIASDD132 pKa = 3.95PAPLALAQPMEE143 pKa = 4.32YY144 pKa = 10.06LGSSAAASGGSDD156 pKa = 2.75AGAAAVGSTAMGEE169 pKa = 4.11LMPMLGAVTFGVIAATSANNATQAQTPNPSPTATGTAPPLVFSISHH215 pKa = 6.48AAGTPHH221 pKa = 7.49VMVVDD226 pKa = 3.8VTLPSTMVAGQTLTFSVAQGNAVLEE251 pKa = 4.4TVNYY255 pKa = 10.44VLTQTDD261 pKa = 3.47INAGSITKK269 pKa = 10.02FLGSNNLSDD278 pKa = 3.9SNYY281 pKa = 10.1QISVNQAGTTALASNTSTFHH301 pKa = 7.52LDD303 pKa = 2.98VTAPSAPQLNLSPNGNGTTVNVSLPIDD330 pKa = 3.72AVVGDD335 pKa = 4.68SLTLTLRR342 pKa = 11.84DD343 pKa = 3.57QTGSAVQTVTQAVTALNLSTHH364 pKa = 6.04TLQLSLNTGALPANTSYY381 pKa = 11.02QALANLTDD389 pKa = 3.22VSGNVSINSTPISMTTPGAPTPGITLTQDD418 pKa = 2.43SGTPGDD424 pKa = 4.73RR425 pKa = 11.84ITNQAGISLSGVLSSGVVEE444 pKa = 4.03YY445 pKa = 10.94SVDD448 pKa = 3.26GGAWTSVYY456 pKa = 8.83TAPTQNGNHH465 pKa = 5.3TVQVRR470 pKa = 11.84QFDD473 pKa = 4.28TNTGHH478 pKa = 6.32VSNPGSLSFTLDD490 pKa = 3.06TLAPAALIPVLANGANGLTNSAALAALTPEE520 pKa = 4.06VGALLEE526 pKa = 3.96YY527 pKa = 10.45RR528 pKa = 11.84VDD530 pKa = 3.46GGAWASSYY538 pKa = 7.73TAPTGDD544 pKa = 3.12IAHH547 pKa = 6.17TVDD550 pKa = 4.48VRR552 pKa = 11.84QTDD555 pKa = 3.27AAGNISAWFTLSFTLDD571 pKa = 3.24TVAPASVTPTLANGATTLTNNAALTALSATEE602 pKa = 3.78VGATVEE608 pKa = 3.97YY609 pKa = 10.42RR610 pKa = 11.84VDD612 pKa = 3.24GGAWGAYY619 pKa = 5.79TAPSAQGAHH628 pKa = 4.7TVEE631 pKa = 4.02ARR633 pKa = 11.84QVDD636 pKa = 3.65AAGNASAASAPLAFTLDD653 pKa = 3.39TQAPVAPGVALSNGNPAGSTTNNGSVSVTGIEE685 pKa = 3.84AGATVEE691 pKa = 4.06YY692 pKa = 10.23QINASGTWLPLVADD706 pKa = 3.84AQGAYY711 pKa = 9.52NVPSPTVPGLSYY723 pKa = 10.56TIDD726 pKa = 3.35VRR728 pKa = 11.84QTDD731 pKa = 3.2AAGNVSTTVGSINFALAGAGSAPTVGLVNDD761 pKa = 3.98TGVVGDD767 pKa = 5.46AITSVGQLQITPAADD782 pKa = 3.26TAPNSTVQEE791 pKa = 4.3FSTDD795 pKa = 3.65GGQTWSTSFTPTSNAVNVVEE815 pKa = 4.32VRR817 pKa = 11.84YY818 pKa = 10.2NNPSDD823 pKa = 3.54TTGNSATLPASFTFTLDD840 pKa = 3.54TQVVAPTLSLQTGTSALTNSSAVTLGGIEE869 pKa = 4.09AGASVEE875 pKa = 4.0YY876 pKa = 9.8STDD879 pKa = 3.14GATWTAQQPVATQGSNTLYY898 pKa = 10.6VRR900 pKa = 11.84QTDD903 pKa = 3.63VAGNVSAASTALSFNLDD920 pKa = 3.44SVAPTAPVVVLTNGATTLTNNAALAALTGVEE951 pKa = 4.27AGALLEE957 pKa = 3.89YY958 pKa = 10.38RR959 pKa = 11.84VDD961 pKa = 3.46GGAWASSYY969 pKa = 7.77TAPTANAAHH978 pKa = 5.9TVDD981 pKa = 4.63VRR983 pKa = 11.84QTDD986 pKa = 3.18AAGNVSPTSSLSFTLDD1002 pKa = 3.01TLAPAALTPVLANGANGLTNSAALAALTPEE1032 pKa = 4.06VGALLEE1038 pKa = 3.96YY1039 pKa = 10.45RR1040 pKa = 11.84VDD1042 pKa = 3.46GGAWASSYY1050 pKa = 7.77TAPTANAAHH1059 pKa = 5.9TVDD1062 pKa = 4.63VRR1064 pKa = 11.84QTDD1067 pKa = 3.18AAGNVSPTSSLSFTLDD1083 pKa = 3.09TVAPAALGLALALANGANGLTNSAALAALTPEE1115 pKa = 4.06VGALLEE1121 pKa = 3.96YY1122 pKa = 10.45RR1123 pKa = 11.84VDD1125 pKa = 3.46GGAWASSYY1133 pKa = 7.77TAPTANAAHH1142 pKa = 5.9TVDD1145 pKa = 4.63VRR1147 pKa = 11.84QTDD1150 pKa = 3.24AAGNVSLASTPLAFTLDD1167 pKa = 3.42TVAPVALTVALLTDD1181 pKa = 3.77SADD1184 pKa = 3.32ATAAPAVFGAQIADD1198 pKa = 3.82KK1199 pKa = 9.75LTNDD1203 pKa = 3.26PTANFVPEE1211 pKa = 4.02TAGNLVEE1218 pKa = 4.21YY1219 pKa = 10.64SVDD1222 pKa = 5.42GINNWQPLSALAPGVLADD1240 pKa = 3.68GAYY1243 pKa = 10.58NLFVRR1248 pKa = 11.84EE1249 pKa = 3.44TDD1251 pKa = 3.05AAGNVTTSATGTYY1264 pKa = 10.39SFTLDD1269 pKa = 3.53TVSTAPTLALNAPILAGGVTYY1290 pKa = 10.66SQTGSFWDD1298 pKa = 3.78PLGGLLTGGTSTSTITDD1315 pKa = 3.41LHH1317 pKa = 7.03PSTEE1321 pKa = 4.3HH1322 pKa = 4.7YY1323 pKa = 9.43TYY1325 pKa = 11.18TDD1327 pKa = 3.2ATTGTPITTDD1337 pKa = 3.35LLGMQNYY1344 pKa = 10.2LLTLGGLINQTTGIGIPVSVTVTQTDD1370 pKa = 3.39AAGNASAPSAPLSFTFDD1387 pKa = 2.9NMTPLPVVGLATDD1400 pKa = 3.8SGRR1403 pKa = 11.84LNDD1406 pKa = 3.57VTTNQPTFSLLATEE1420 pKa = 4.6AQTTEE1425 pKa = 4.32AYY1427 pKa = 10.73SFDD1430 pKa = 3.14GMTWTSIPTLVNGTFTPTGLPIPAQVATPTAVPFWVQQTDD1470 pKa = 2.92ATGNVGTFMTTFMVDD1485 pKa = 3.15NFVAAPTVQATDD1497 pKa = 3.2STGALVGSGTLVSSGTLTLTGEE1519 pKa = 4.23TSLAPTYY1526 pKa = 10.24QVSTDD1531 pKa = 3.92SGITWVDD1538 pKa = 3.1SSIYY1542 pKa = 10.22TPTAMGANNILVHH1555 pKa = 6.55QIDD1558 pKa = 3.83DD1559 pKa = 4.05AGNISAATTFNLNLQGTVTNVTTGLSSAEE1588 pKa = 4.0AAGVSPFITLPTMSLTDD1605 pKa = 3.33SGIAGDD1611 pKa = 5.08HH1612 pKa = 5.61ITSNATVTLNAANLGGNVDD1631 pKa = 3.83TLSYY1635 pKa = 10.81SLNGGLWTAYY1645 pKa = 9.42TPGSSLPAVEE1655 pKa = 4.86GLNSLLVKK1663 pKa = 10.64EE1664 pKa = 5.0SVQSGAIGTLATHH1677 pKa = 6.4EE1678 pKa = 4.34TPIIPMSFTLDD1689 pKa = 3.47TVFPTAGATANNIAEE1704 pKa = 5.15LYY1706 pKa = 9.1TSASSGLSTAVVRR1719 pKa = 11.84VTYY1722 pKa = 10.78SEE1724 pKa = 4.18AVSVNAATLNFSGGGAAGGYY1744 pKa = 6.78TSQVVTSGASLDD1756 pKa = 3.95GAVSLGVQVDD1766 pKa = 4.12GSTVIEE1772 pKa = 4.06VRR1774 pKa = 11.84LAEE1777 pKa = 4.32TTLHH1781 pKa = 6.81AFTTAQVVPSVSLLITDD1798 pKa = 4.75LAGNQATVPIAVTNNPAPVFADD1820 pKa = 3.34WNLGMLPP1827 pKa = 3.87

Molecular weight: 183.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4Z5K8|I4Z5K8_9BURK cAMP-binding protein OS=Leptothrix ochracea L12 OX=735332 GN=LepocDRAFT_00002310 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.05PLFHH6 pKa = 7.04VLLQFKK12 pKa = 9.95EE13 pKa = 4.27KK14 pKa = 10.7SRR16 pKa = 11.84LNTACCTAGCCVITSLHH33 pKa = 5.97RR34 pKa = 11.84RR35 pKa = 11.84PIRR38 pKa = 11.84RR39 pKa = 11.84MVLLSWINLQILRR52 pKa = 11.84SCHH55 pKa = 4.79QNRR58 pKa = 11.84VLTTNSNDD66 pKa = 3.53SCRR69 pKa = 11.84HH70 pKa = 5.42RR71 pKa = 11.84SNHH74 pKa = 4.87NNQRR78 pKa = 11.84PRR80 pKa = 11.84NHH82 pKa = 5.96QPNHH86 pKa = 6.58LYY88 pKa = 10.65LPPSKK93 pKa = 10.9GLFNTLFADD102 pKa = 3.94SSITGFFCILLIHH115 pKa = 7.22GYY117 pKa = 6.93TT118 pKa = 3.49

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

531

0

531

150642

20

1827

283.7

30.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.473 ± 0.14

1.069 ± 0.059

5.272 ± 0.074

5.006 ± 0.139

3.397 ± 0.074

7.956 ± 0.138

2.618 ± 0.073

4.703 ± 0.085

3.316 ± 0.116

10.7 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.068

2.861 ± 0.111

5.192 ± 0.078

4.257 ± 0.073

6.484 ± 0.164

6.045 ± 0.123

5.742 ± 0.163

7.586 ± 0.101

1.587 ± 0.054

2.212 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski