Proteiniclasticum ruminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Proteiniclasticum

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2937 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5F4B0|A0A1I5F4B0_9CLOT Transposase OS=Proteiniclasticum ruminis OX=398199 GN=SAMN04488695_12811 PE=3 SV=1
MM1 pKa = 7.61IEE3 pKa = 3.89NLEE6 pKa = 4.06IEE8 pKa = 4.74EE9 pKa = 4.27FTTITKK15 pKa = 10.61AFVIDD20 pKa = 4.67IIFEE24 pKa = 4.29FYY26 pKa = 11.05LCIIVTNSS34 pKa = 2.59

Molecular weight: 4.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4XQ68|A0A1I4XQ68_9CLOT HDIG domain-containing protein OS=Proteiniclasticum ruminis OX=398199 GN=SAMN04488695_101117 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84EE4 pKa = 3.64NSSRR8 pKa = 11.84DD9 pKa = 3.46FKK11 pKa = 11.28RR12 pKa = 11.84NNPRR16 pKa = 11.84GRR18 pKa = 11.84RR19 pKa = 11.84AKK21 pKa = 10.38RR22 pKa = 11.84KK23 pKa = 8.58VCSFCVDD30 pKa = 3.26KK31 pKa = 11.23VDD33 pKa = 4.78HH34 pKa = 7.12IDD36 pKa = 3.58YY37 pKa = 10.88KK38 pKa = 11.22DD39 pKa = 3.4VSKK42 pKa = 10.65LRR44 pKa = 11.84KK45 pKa = 9.84YY46 pKa = 8.52ITEE49 pKa = 4.01RR50 pKa = 11.84GKK52 pKa = 10.04ILPRR56 pKa = 11.84RR57 pKa = 11.84ISGTCAKK64 pKa = 9.88HH65 pKa = 6.02QRR67 pKa = 11.84TLTDD71 pKa = 4.58AIKK74 pKa = 10.34RR75 pKa = 11.84SRR77 pKa = 11.84NIALLPFTQDD87 pKa = 2.5

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2937

0

2937

937308

21

2943

319.1

35.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.473 ± 0.048

0.694 ± 0.015

5.193 ± 0.035

8.54 ± 0.057

4.452 ± 0.036

7.019 ± 0.044

1.892 ± 0.021

7.309 ± 0.044

7.373 ± 0.043

10.398 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.139 ± 0.023

4.044 ± 0.032

3.314 ± 0.023

2.692 ± 0.023

4.326 ± 0.031

6.212 ± 0.032

5.223 ± 0.031

7.061 ± 0.04

0.699 ± 0.016

3.948 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski