Chlorella sorokiniana (Freshwater green alga)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Chlorellales; Chlorellaceae; Chlorella clade; Chlorella

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6U2K8|A0A2P6U2K8_CHLSO Ankyrin repeat isoform C OS=Chlorella sorokiniana OX=3076 GN=C2E21_1100 PE=3 SV=1
MM1 pKa = 6.92VAALVAGGADD11 pKa = 2.75INAPVEE17 pKa = 4.29ADD19 pKa = 3.57PCHH22 pKa = 6.68LPAYY26 pKa = 8.71TLLHH30 pKa = 5.65WAAYY34 pKa = 9.66SNAVDD39 pKa = 5.28AIQVLAACGADD50 pKa = 3.59LEE52 pKa = 4.79VRR54 pKa = 11.84DD55 pKa = 4.1ATVDD59 pKa = 3.45NSSLGRR65 pKa = 11.84WTEE68 pKa = 3.9FVGGGQTPLMVAAANGHH85 pKa = 5.13VEE87 pKa = 4.02AVEE90 pKa = 3.8ALIAAGCNANAGTTDD105 pKa = 3.06WGSWSPKK112 pKa = 9.54GATALEE118 pKa = 4.32FAVVGPGSGCQPGAQNPEE136 pKa = 3.88GLPAVVNALLAAGAVDD152 pKa = 3.72VEE154 pKa = 4.63YY155 pKa = 10.99ALEE158 pKa = 4.36AALHH162 pKa = 5.3RR163 pKa = 11.84HH164 pKa = 5.67ASEE167 pKa = 4.03EE168 pKa = 3.81MLAALEE174 pKa = 4.25AAVDD178 pKa = 3.9DD179 pKa = 5.23DD180 pKa = 4.78SEE182 pKa = 4.33EE183 pKa = 5.15DD184 pKa = 3.44EE185 pKa = 5.37DD186 pKa = 4.57EE187 pKa = 4.48EE188 pKa = 5.4SGSDD192 pKa = 3.51SDD194 pKa = 4.24

Molecular weight: 19.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6TEX9|A0A2P6TEX9_CHLSO Metallothiol transferase isoform B OS=Chlorella sorokiniana OX=3076 GN=C2E21_8300 PE=4 SV=1
MM1 pKa = 7.48AALAPVQSLLHH12 pKa = 6.63LDD14 pKa = 3.63NALVAIPEE22 pKa = 4.27DD23 pKa = 3.88AACCSTSQPAAPARR37 pKa = 11.84WVDD40 pKa = 3.5HH41 pKa = 6.12EE42 pKa = 4.46RR43 pKa = 11.84LLAAGEE49 pKa = 3.97ARR51 pKa = 11.84VRR53 pKa = 11.84PLRR56 pKa = 11.84KK57 pKa = 9.17CASVPTLHH65 pKa = 7.03SMQEE69 pKa = 3.81ACGEE73 pKa = 4.29SEE75 pKa = 3.84GMEE78 pKa = 4.15VYY80 pKa = 10.76VILRR84 pKa = 11.84PFKK87 pKa = 10.56EE88 pKa = 4.0FGGGLFRR95 pKa = 11.84RR96 pKa = 11.84LPKK99 pKa = 9.89RR100 pKa = 11.84VKK102 pKa = 10.71NGVRR106 pKa = 11.84DD107 pKa = 3.74CGLCHH112 pKa = 6.21YY113 pKa = 10.44LAVFKK118 pKa = 11.05QKK120 pKa = 10.66DD121 pKa = 3.48GSLVQFDD128 pKa = 4.0FGPRR132 pKa = 11.84GGDD135 pKa = 2.76IHH137 pKa = 6.42VARR140 pKa = 11.84GPFAFLSKK148 pKa = 10.71SADD151 pKa = 2.87GKK153 pKa = 7.61MQRR156 pKa = 11.84LVPGEE161 pKa = 3.84VRR163 pKa = 11.84EE164 pKa = 4.01RR165 pKa = 11.84RR166 pKa = 11.84LMRR169 pKa = 11.84LPDD172 pKa = 2.78AHH174 pKa = 6.57MYY176 pKa = 9.76VGRR179 pKa = 11.84TPLSLEE185 pKa = 5.81DD186 pKa = 3.2IRR188 pKa = 11.84AWNALQEE195 pKa = 4.31QGSMHH200 pKa = 6.49YY201 pKa = 9.63EE202 pKa = 3.8LHH204 pKa = 7.27RR205 pKa = 11.84NDD207 pKa = 3.07CRR209 pKa = 11.84HH210 pKa = 6.43YY211 pKa = 10.22INCLVKK217 pKa = 9.05YY218 pKa = 5.39TTGRR222 pKa = 11.84EE223 pKa = 3.96QAASACLAAQWQRR236 pKa = 11.84ARR238 pKa = 11.84QLGTYY243 pKa = 9.94GLATSVVRR251 pKa = 11.84LTQFFTDD258 pKa = 4.1LANWGKK264 pKa = 9.61VQLIGNVSMYY274 pKa = 11.33GMMALSGQKK283 pKa = 9.92VLARR287 pKa = 11.84LPALLPGAKK296 pKa = 9.89AKK298 pKa = 9.62LQPVVSKK305 pKa = 11.05ALAGGVKK312 pKa = 9.92RR313 pKa = 11.84ALTGPVRR320 pKa = 11.84TAIARR325 pKa = 11.84KK326 pKa = 7.93PVVVGTAAMATLAASSSQAPMIKK349 pKa = 8.81EE350 pKa = 4.31TVSVGARR357 pKa = 11.84VARR360 pKa = 11.84AAQATIAAASTAVTRR375 pKa = 11.84ASAATVRR382 pKa = 11.84TTSQAAVAVGSITRR396 pKa = 11.84GAVALAAGRR405 pKa = 11.84PMPLALEE412 pKa = 4.43WGRR415 pKa = 11.84AASPPRR421 pKa = 11.84ASLGMRR427 pKa = 11.84AADD430 pKa = 3.62RR431 pKa = 11.84SQRR434 pKa = 11.84LALAITSAARR444 pKa = 11.84RR445 pKa = 3.73

Molecular weight: 47.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9482

719

10201

6715017

54

18393

658.3

69.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.604 ± 0.05

1.646 ± 0.015

4.438 ± 0.013

5.941 ± 0.026

2.63 ± 0.012

8.821 ± 0.022

2.036 ± 0.01

2.523 ± 0.014

3.157 ± 0.019

10.146 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.895 ± 0.008

2.043 ± 0.012

6.398 ± 0.027

5.682 ± 0.028

6.359 ± 0.02

6.759 ± 0.02

4.428 ± 0.026

6.162 ± 0.017

1.416 ± 0.008

1.917 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski