Amino acid dipepetide frequency for Chlorella sorokiniana (Freshwater green alga)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	41.143AlaAla: 41.143 ± 0.336	2.227AlaCys: 2.227 ± 0.04	6.242AlaAsp: 6.242 ± 0.036	9.262AlaGlu: 9.262 ± 0.08	3.322AlaPhe: 3.322 ± 0.029	13.84AlaGly: 13.84 ± 0.066	2.313AlaHis: 2.313 ± 0.022	3.073AlaIle: 3.073 ± 0.023	3.975AlaLys: 3.975 ± 0.037	13.561AlaLeu: 13.561 ± 0.078	2.557AlaMet: 2.557 ± 0.024	2.399AlaAsn: 2.399 ± 0.025	8.68AlaPro: 8.68 ± 0.059	7.139AlaGln: 7.139 ± 0.06	7.25AlaArg: 7.25 ± 0.044	9.098AlaSer: 9.098 ± 0.056	6.302AlaThr: 6.302 ± 0.042	9.789AlaVal: 9.789 ± 0.058	1.724AlaTrp: 1.724 ± 0.019	2.142AlaTyr: 2.142 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	1.751CysAla: 1.751 ± 0.042	0.506CysCys: 0.506 ± 0.019	0.744CysAsp: 0.744 ± 0.013	0.718CysGlu: 0.718 ± 0.014	0.524CysPhe: 0.524 ± 0.01	1.507CysGly: 1.507 ± 0.023	0.337CysHis: 0.337 ± 0.007	0.548CysIle: 0.548 ± 0.008	0.609CysLys: 0.609 ± 0.011	1.678CysLeu: 1.678 ± 0.022	0.347CysMet: 0.347 ± 0.008	0.473CysAsn: 0.473 ± 0.01	0.993CysPro: 0.993 ± 0.02	0.647CysGln: 0.647 ± 0.012	1.083CysArg: 1.083 ± 0.014	1.355CysSer: 1.355 ± 0.019	1.077CysThr: 1.077 ± 0.029	0.912CysVal: 0.912 ± 0.015	0.278CysTrp: 0.278 ± 0.006	0.374CysTyr: 0.374 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	6.222AspAla: 6.222 ± 0.035	0.787AspCys: 0.787 ± 0.015	2.437AspAsp: 2.437 ± 0.023	3.176AspGlu: 3.176 ± 0.028	1.389AspPhe: 1.389 ± 0.017	4.236AspGly: 4.236 ± 0.031	0.706AspHis: 0.706 ± 0.012	1.471AspIle: 1.471 ± 0.016	1.528AspLys: 1.528 ± 0.02	4.158AspLeu: 4.158 ± 0.03	0.944AspMet: 0.944 ± 0.014	0.942AspAsn: 0.942 ± 0.014	2.633AspPro: 2.633 ± 0.023	1.429AspGln: 1.429 ± 0.015	2.345AspArg: 2.345 ± 0.022	2.99AspSer: 2.99 ± 0.021	1.967AspThr: 1.967 ± 0.021	3.155AspVal: 3.155 ± 0.026	0.822AspTrp: 0.822 ± 0.014	1.042AspTyr: 1.042 ± 0.015	0.0AspXaa: 0.0 ± 0.0
Glu	9.699GluAla: 9.699 ± 0.077	0.757GluCys: 0.757 ± 0.012	2.944GluAsp: 2.944 ± 0.027	6.173GluGlu: 6.173 ± 0.076	1.312GluPhe: 1.312 ± 0.016	5.087GluGly: 5.087 ± 0.032	1.178GluHis: 1.178 ± 0.014	1.308GluIle: 1.308 ± 0.018	1.688GluLys: 1.688 ± 0.026	6.261GluLeu: 6.261 ± 0.041	1.062GluMet: 1.062 ± 0.015	0.865GluAsn: 0.865 ± 0.014	2.858GluPro: 2.858 ± 0.026	4.131GluGln: 4.131 ± 0.042	4.272GluArg: 4.272 ± 0.035	2.394GluSer: 2.394 ± 0.021	1.772GluThr: 1.772 ± 0.02	3.835GluVal: 3.835 ± 0.023	0.796GluTrp: 0.796 ± 0.012	1.017GluTyr: 1.017 ± 0.012	0.0GluXaa: 0.0 ± 0.0
Phe	2.909PheAla: 2.909 ± 0.024	0.528PheCys: 0.528 ± 0.01	1.513PheAsp: 1.513 ± 0.017	1.542PheGlu: 1.542 ± 0.016	0.959PhePhe: 0.959 ± 0.016	2.39PheGly: 2.39 ± 0.028	0.526PheHis: 0.526 ± 0.009	0.87PheIle: 0.87 ± 0.014	1.039PheLys: 1.039 ± 0.016	2.454PheLeu: 2.454 ± 0.02	0.584PheMet: 0.584 ± 0.01	0.837PheAsn: 0.837 ± 0.013	1.289PhePro: 1.289 ± 0.015	1.06PheGln: 1.06 ± 0.013	1.465PheArg: 1.465 ± 0.015	1.891PheSer: 1.891 ± 0.018	1.4PheThr: 1.4 ± 0.021	1.906PheVal: 1.906 ± 0.018	0.421PheTrp: 0.421 ± 0.008	0.717PheTyr: 0.717 ± 0.012	0.0PheXaa: 0.0 ± 0.0
Gly	11.306GlyAla: 11.306 ± 0.052	1.436GlyCys: 1.436 ± 0.02	3.649GlyAsp: 3.649 ± 0.028	4.548GlyGlu: 4.548 ± 0.031	2.228GlyPhe: 2.228 ± 0.021	12.11GlyGly: 12.11 ± 0.096	1.573GlyHis: 1.573 ± 0.016	2.217GlyIle: 2.217 ± 0.022	3.019GlyLys: 3.019 ± 0.028	7.45GlyLeu: 7.45 ± 0.043	1.904GlyMet: 1.904 ± 0.025	1.783GlyAsn: 1.783 ± 0.022	4.276GlyPro: 4.276 ± 0.033	3.928GlyGln: 3.928 ± 0.03	5.619GlyArg: 5.619 ± 0.032	8.968GlySer: 8.968 ± 0.062	4.279GlyThr: 4.279 ± 0.039	4.865GlyVal: 4.865 ± 0.033	1.373GlyTrp: 1.373 ± 0.018	1.684GlyTyr: 1.684 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	2.419HisAla: 2.419 ± 0.024	0.422HisCys: 0.422 ± 0.009	0.812HisAsp: 0.812 ± 0.012	1.016HisGlu: 1.016 ± 0.013	0.661HisPhe: 0.661 ± 0.01	1.634HisGly: 1.634 ± 0.016	0.61HisHis: 0.61 ± 0.013	0.675HisIle: 0.675 ± 0.01	0.695HisLys: 0.695 ± 0.013	2.208HisLeu: 2.208 ± 0.023	0.488HisMet: 0.488 ± 0.009	0.49HisAsn: 0.49 ± 0.009	1.417HisPro: 1.417 ± 0.02	0.852HisGln: 0.852 ± 0.011	1.373HisArg: 1.373 ± 0.021	1.45HisSer: 1.45 ± 0.016	1.027HisThr: 1.027 ± 0.014	1.251HisVal: 1.251 ± 0.014	0.358HisTrp: 0.358 ± 0.007	0.502HisTyr: 0.502 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	2.978IleAla: 2.978 ± 0.024	0.49IleCys: 0.49 ± 0.009	1.469IleAsp: 1.469 ± 0.019	1.547IleGlu: 1.547 ± 0.018	0.825IlePhe: 0.825 ± 0.012	1.868IleGly: 1.868 ± 0.02	0.509IleHis: 0.509 ± 0.01	0.931IleIle: 0.931 ± 0.017	1.116IleLys: 1.116 ± 0.017	2.156IleLeu: 2.156 ± 0.02	0.58IleMet: 0.58 ± 0.01	0.841IleAsn: 0.841 ± 0.013	1.36IlePro: 1.36 ± 0.016	1.031IleGln: 1.031 ± 0.013	1.438IleArg: 1.438 ± 0.018	1.759IleSer: 1.759 ± 0.019	1.494IleThr: 1.494 ± 0.019	1.863IleVal: 1.863 ± 0.022	0.348IleTrp: 0.348 ± 0.007	0.628IleTyr: 0.628 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	4.306LysAla: 4.306 ± 0.036	0.467LysCys: 0.467 ± 0.013	1.43LysAsp: 1.43 ± 0.016	2.128LysGlu: 2.128 ± 0.023	0.758LysPhe: 0.758 ± 0.014	2.393LysGly: 2.393 ± 0.025	0.696LysHis: 0.696 ± 0.01	0.827LysIle: 0.827 ± 0.014	1.772LysLys: 1.772 ± 0.028	3.223LysLeu: 3.223 ± 0.026	0.611LysMet: 0.611 ± 0.011	0.654LysAsn: 0.654 ± 0.012	1.992LysPro: 1.992 ± 0.022	1.925LysGln: 1.925 ± 0.017	2.459LysArg: 2.459 ± 0.022	1.503LysSer: 1.503 ± 0.016	1.257LysThr: 1.257 ± 0.015	1.999LysVal: 1.999 ± 0.022	0.455LysTrp: 0.455 ± 0.01	0.719LysTyr: 0.719 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	14.237LeuAla: 14.237 ± 0.08	1.638LeuCys: 1.638 ± 0.025	4.634LeuAsp: 4.634 ± 0.028	6.053LeuGlu: 6.053 ± 0.041	2.598LeuPhe: 2.598 ± 0.024	7.271LeuGly: 7.271 ± 0.043	2.514LeuHis: 2.514 ± 0.026	2.163LeuIle: 2.163 ± 0.025	3.017LeuLys: 3.017 ± 0.027	12.394LeuLeu: 12.394 ± 0.083	1.797LeuMet: 1.797 ± 0.021	2.057LeuAsn: 2.057 ± 0.02	7.521LeuPro: 7.521 ± 0.044	6.108LeuGln: 6.108 ± 0.039	7.292LeuArg: 7.292 ± 0.04	6.376LeuSer: 6.376 ± 0.036	4.551LeuThr: 4.551 ± 0.036	6.151LeuVal: 6.151 ± 0.033	1.28LeuTrp: 1.28 ± 0.018	1.806LeuTyr: 1.806 ± 0.018	0.0LeuXaa: 0.0 ± 0.0
Met	2.729MetAla: 2.729 ± 0.025	0.261MetCys: 0.261 ± 0.007	0.907MetAsp: 0.907 ± 0.012	1.128MetGlu: 1.128 ± 0.013	0.516MetPhe: 0.516 ± 0.009	1.469MetGly: 1.469 ± 0.019	0.474MetHis: 0.474 ± 0.009	0.439MetIle: 0.439 ± 0.01	0.555MetLys: 0.555 ± 0.011	2.153MetLeu: 2.153 ± 0.023	0.421MetMet: 0.421 ± 0.01	0.381MetAsn: 0.381 ± 0.008	1.305MetPro: 1.305 ± 0.017	1.219MetGln: 1.219 ± 0.016	1.357MetArg: 1.357 ± 0.016	1.091MetSer: 1.091 ± 0.015	0.756MetThr: 0.756 ± 0.012	1.196MetVal: 1.196 ± 0.014	0.245MetTrp: 0.245 ± 0.007	0.348MetTyr: 0.348 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.294AsnAla: 2.294 ± 0.02	0.467AsnCys: 0.467 ± 0.019	0.871AsnAsp: 0.871 ± 0.012	0.998AsnGlu: 0.998 ± 0.014	0.69AsnPhe: 0.69 ± 0.014	2.018AsnGly: 2.018 ± 0.024	0.359AsnHis: 0.359 ± 0.009	0.815AsnIle: 0.815 ± 0.012	0.795AsnLys: 0.795 ± 0.013	1.987AsnLeu: 1.987 ± 0.019	0.458AsnMet: 0.458 ± 0.009	0.643AsnAsn: 0.643 ± 0.016	1.417AsnPro: 1.417 ± 0.022	0.735AsnGln: 0.735 ± 0.012	1.121AsnArg: 1.121 ± 0.014	1.374AsnSer: 1.374 ± 0.018	1.116AsnThr: 1.116 ± 0.017	1.403AsnVal: 1.403 ± 0.018	0.361AsnTrp: 0.361 ± 0.008	0.508AsnTyr: 0.508 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	11.26ProAla: 11.26 ± 0.081	0.798ProCys: 0.798 ± 0.015	2.775ProAsp: 2.775 ± 0.023	3.593ProGlu: 3.593 ± 0.024	1.543ProPhe: 1.543 ± 0.018	5.572ProGly: 5.572 ± 0.044	1.235ProHis: 1.235 ± 0.014	1.299ProIle: 1.299 ± 0.013	1.654ProLys: 1.654 ± 0.018	5.867ProLeu: 5.867 ± 0.037	0.927ProMet: 0.927 ± 0.012	1.212ProAsn: 1.212 ± 0.019	6.835ProPro: 6.835 ± 0.087	3.073ProGln: 3.073 ± 0.033	3.268ProArg: 3.268 ± 0.025	4.781ProSer: 4.781 ± 0.039	3.003ProThr: 3.003 ± 0.029	3.476ProVal: 3.476 ± 0.026	0.783ProTrp: 0.783 ± 0.012	1.022ProTyr: 1.022 ± 0.015	0.0ProXaa: 0.0 ± 0.0
Gln	7.932GlnAla: 7.932 ± 0.065	0.664GlnCys: 0.664 ± 0.012	1.705GlnAsp: 1.705 ± 0.016	3.097GlnGlu: 3.097 ± 0.027	1.081GlnPhe: 1.081 ± 0.013	3.484GlnGly: 3.484 ± 0.028	1.538GlnHis: 1.538 ± 0.02	0.953GlnIle: 0.953 ± 0.013	1.195GlnLys: 1.195 ± 0.015	7.048GlnLeu: 7.048 ± 0.048	0.877GlnMet: 0.877 ± 0.012	0.641GlnAsn: 0.641 ± 0.01	4.433GlnPro: 4.433 ± 0.053	9.704GlnGln: 9.704 ± 0.13	4.686GlnArg: 4.686 ± 0.037	1.971GlnSer: 1.971 ± 0.017	1.54GlnThr: 1.54 ± 0.017	2.656GlnVal: 2.656 ± 0.021	0.696GlnTrp: 0.696 ± 0.012	0.921GlnTyr: 0.921 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	7.173ArgAla: 7.173 ± 0.043	1.178ArgCys: 1.178 ± 0.015	2.526ArgAsp: 2.526 ± 0.026	3.536ArgGlu: 3.536 ± 0.035	1.815ArgPhe: 1.815 ± 0.02	4.696ArgGly: 4.696 ± 0.032	1.609ArgHis: 1.609 ± 0.018	1.768ArgIle: 1.768 ± 0.02	2.248ArgLys: 2.248 ± 0.022	7.468ArgLeu: 7.468 ± 0.044	1.316ArgMet: 1.316 ± 0.015	1.221ArgAsn: 1.221 ± 0.014	3.835ArgPro: 3.835 ± 0.028	4.305ArgGln: 4.305 ± 0.034	6.194ArgArg: 6.194 ± 0.049	4.24ArgSer: 4.24 ± 0.031	2.563ArgThr: 2.563 ± 0.018	3.54ArgVal: 3.54 ± 0.026	1.055ArgTrp: 1.055 ± 0.014	1.302ArgTyr: 1.302 ± 0.014	0.0ArgXaa: 0.0 ± 0.0
Ser	8.222SerAla: 8.222 ± 0.053	1.135SerCys: 1.135 ± 0.019	2.941SerAsp: 2.941 ± 0.023	3.211SerGlu: 3.211 ± 0.025	1.863SerPhe: 1.863 ± 0.018	7.771SerGly: 7.771 ± 0.06	1.123SerHis: 1.123 ± 0.014	1.815SerIle: 1.815 ± 0.02	2.215SerLys: 2.215 ± 0.022	6.583SerLeu: 6.583 ± 0.045	1.362SerMet: 1.362 ± 0.014	1.546SerAsn: 1.546 ± 0.019	4.524SerPro: 4.524 ± 0.051	2.639SerGln: 2.639 ± 0.027	3.836SerArg: 3.836 ± 0.03	7.612SerSer: 7.612 ± 0.076	3.259SerThr: 3.259 ± 0.029	3.636SerVal: 3.636 ± 0.027	1.01SerTrp: 1.01 ± 0.014	1.285SerTyr: 1.285 ± 0.017	0.0SerXaa: 0.0 ± 0.0
Thr	7.02ThrAla: 7.02 ± 0.064	0.893ThrCys: 0.893 ± 0.024	1.95ThrAsp: 1.95 ± 0.018	2.013ThrGlu: 2.013 ± 0.02	1.392ThrPhe: 1.392 ± 0.02	3.859ThrGly: 3.859 ± 0.033	0.822ThrHis: 0.822 ± 0.012	1.421ThrIle: 1.421 ± 0.017	1.306ThrLys: 1.306 ± 0.016	4.536ThrLeu: 4.536 ± 0.033	0.803ThrMet: 0.803 ± 0.011	1.069ThrAsn: 1.069 ± 0.02	3.053ThrPro: 3.053 ± 0.03	1.748ThrGln: 1.748 ± 0.019	2.183ThrArg: 2.183 ± 0.02	3.1ThrSer: 3.1 ± 0.032	2.307ThrThr: 2.307 ± 0.028	3.158ThrVal: 3.158 ± 0.032	0.669ThrTrp: 0.669 ± 0.012	0.973ThrTyr: 0.973 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	8.911ValAla: 8.911 ± 0.05	1.088ValCys: 1.088 ± 0.015	3.044ValAsp: 3.044 ± 0.025	3.738ValGlu: 3.738 ± 0.026	1.77ValPhe: 1.77 ± 0.02	4.306ValGly: 4.306 ± 0.034	1.44ValHis: 1.44 ± 0.014	1.637ValIle: 1.637 ± 0.02	2.011ValLys: 2.011 ± 0.021	6.754ValLeu: 6.754 ± 0.038	1.183ValMet: 1.183 ± 0.015	1.374ValAsn: 1.374 ± 0.018	3.985ValPro: 3.985 ± 0.026	3.361ValGln: 3.361 ± 0.024	3.73ValArg: 3.73 ± 0.029	3.442ValSer: 3.442 ± 0.027	2.962ValThr: 2.962 ± 0.029	4.721ValVal: 4.721 ± 0.037	0.872ValTrp: 0.872 ± 0.013	1.296ValTyr: 1.296 ± 0.015	0.0ValXaa: 0.0 ± 0.0
Trp	1.574TrpAla: 1.574 ± 0.017	0.25TrpCys: 0.25 ± 0.006	0.718TrpAsp: 0.718 ± 0.013	0.872TrpGlu: 0.872 ± 0.011	0.388TrpPhe: 0.388 ± 0.008	1.066TrpGly: 1.066 ± 0.016	0.381TrpHis: 0.381 ± 0.007	0.334TrpIle: 0.334 ± 0.008	0.439TrpLys: 0.439 ± 0.008	1.601TrpLeu: 1.601 ± 0.02	0.297TrpMet: 0.297 ± 0.006	0.346TrpAsn: 0.346 ± 0.008	0.693TrpPro: 0.693 ± 0.011	0.995TrpGln: 0.995 ± 0.013	1.314TrpArg: 1.314 ± 0.016	0.85TrpSer: 0.85 ± 0.013	0.584TrpThr: 0.584 ± 0.011	0.873TrpVal: 0.873 ± 0.011	0.291TrpTrp: 0.291 ± 0.007	0.293TrpTyr: 0.293 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.954TyrAla: 1.954 ± 0.018	0.466TyrCys: 0.466 ± 0.009	1.066TyrAsp: 1.066 ± 0.014	1.061TyrGlu: 1.061 ± 0.013	0.668TyrPhe: 0.668 ± 0.011	1.64TyrGly: 1.64 ± 0.018	0.419TyrHis: 0.419 ± 0.009	0.666TyrIle: 0.666 ± 0.011	0.698TyrLys: 0.698 ± 0.011	1.917TyrLeu: 1.917 ± 0.018	0.432TyrMet: 0.432 ± 0.009	0.657TyrAsn: 0.657 ± 0.01	0.878TyrPro: 0.878 ± 0.013	0.792TyrGln: 0.792 ± 0.013	1.304TyrArg: 1.304 ± 0.02	1.341TyrSer: 1.341 ± 0.017	1.058TyrThr: 1.058 ± 0.018	1.239TyrVal: 1.239 ± 0.014	0.324TyrTrp: 0.324 ± 0.007	0.589TyrTyr: 0.589 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10201 proteins (6715017 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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