Rhodobacter veldkampii DSM 11550

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Bieblia; Bieblia veldkampii

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4JL83|A0A2T4JL83_9RHOB Alpha-ketoglutarate dehydrogenase OS=Rhodobacter veldkampii DSM 11550 OX=1185920 GN=C5F46_03440 PE=4 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84EE3 pKa = 3.93GDD5 pKa = 4.12DD6 pKa = 4.01LQIMLADD13 pKa = 3.72GRR15 pKa = 11.84TITLSGYY22 pKa = 9.45YY23 pKa = 9.44DD24 pKa = 3.27AHH26 pKa = 5.93ARR28 pKa = 11.84LYY30 pKa = 10.29ISSDD34 pKa = 3.57GEE36 pKa = 4.16LTPVTLEE43 pKa = 4.23DD44 pKa = 3.75GGNGVIYY51 pKa = 10.47ADD53 pKa = 3.62YY54 pKa = 10.53GQVEE58 pKa = 5.18VIGKK62 pKa = 8.39WSPNDD67 pKa = 3.12QLAFLGGDD75 pKa = 4.11EE76 pKa = 4.4ILSPAGDD83 pKa = 4.26DD84 pKa = 3.72TTGMAMFAPAMLGMGGLGAAGAGLAGLGLLGLGGGGSKK122 pKa = 10.79GGGDD126 pKa = 3.76DD127 pKa = 3.56TTPGGGEE134 pKa = 3.9VDD136 pKa = 4.54PIVPTVDD143 pKa = 3.2TPDD146 pKa = 3.37AQTTLTTNTEE156 pKa = 3.95DD157 pKa = 3.41PAATVSGTGEE167 pKa = 4.0PGSTVEE173 pKa = 5.4VVLGDD178 pKa = 3.48QTLEE182 pKa = 4.02TTIDD186 pKa = 3.67EE187 pKa = 4.87DD188 pKa = 4.39GTWSVTFEE196 pKa = 5.27DD197 pKa = 4.46DD198 pKa = 3.67TFPSDD203 pKa = 3.8GDD205 pKa = 3.68LTSTVDD211 pKa = 3.12VTAPDD216 pKa = 3.61GTEE219 pKa = 3.91YY220 pKa = 10.86TLDD223 pKa = 4.02GPDD226 pKa = 4.19FLIDD230 pKa = 3.52MTPPAVVITEE240 pKa = 4.54GALSTGDD247 pKa = 3.51VEE249 pKa = 5.62NLSEE253 pKa = 4.25YY254 pKa = 10.56QDD256 pKa = 4.35GITVRR261 pKa = 11.84GTGEE265 pKa = 3.61AGAAIVVAVAGHH277 pKa = 6.07AQATTVAADD286 pKa = 4.21GSWSVTFTTEE296 pKa = 3.57QMPAGTYY303 pKa = 9.87SEE305 pKa = 4.68AMTVTATDD313 pKa = 3.11AVGNVTTLNDD323 pKa = 3.66TLVVDD328 pKa = 4.8TEE330 pKa = 4.25ASVAFSSASVTGDD343 pKa = 3.36NIVNADD349 pKa = 3.56EE350 pKa = 4.54AQTGFALSGTSEE362 pKa = 4.25PGSVSVRR369 pKa = 11.84IEE371 pKa = 3.94LGGTSYY377 pKa = 11.36SATPAADD384 pKa = 3.93GTWSIALQDD393 pKa = 3.54VALATGVHH401 pKa = 5.79TATVTATDD409 pKa = 3.81ANGNTASATRR419 pKa = 11.84AISFDD424 pKa = 3.32TDD426 pKa = 3.29TSVSFDD432 pKa = 3.19SAQAGGNNLLNAAEE446 pKa = 4.14RR447 pKa = 11.84SAGLVLTGTAEE458 pKa = 4.07PGATVVVAFEE468 pKa = 4.34QGSHH472 pKa = 4.92TVTAGADD479 pKa = 3.91GHH481 pKa = 6.36WSASFAASEE490 pKa = 4.34VRR492 pKa = 11.84TGTYY496 pKa = 10.45SSTATVTATDD506 pKa = 3.11IAGNVATASHH516 pKa = 7.51TINVDD521 pKa = 3.4TEE523 pKa = 4.52VQNFTHH529 pKa = 6.99TSPTANAIVADD540 pKa = 4.75DD541 pKa = 3.47IVNARR546 pKa = 11.84EE547 pKa = 3.83AAGGLVVTGTVEE559 pKa = 3.84PGATVTVQLASGSVIPAINLGNGSWTATIPAAQLPAVEE597 pKa = 4.54TDD599 pKa = 3.1NVTLTVRR606 pKa = 11.84ATDD609 pKa = 3.59VNGNTATQTSSIDD622 pKa = 3.34FDD624 pKa = 3.78PVVRR628 pKa = 11.84NFAPNATVAGDD639 pKa = 4.26GIVNAQEE646 pKa = 4.19GADD649 pKa = 3.53GFVISGAVEE658 pKa = 3.87PGSRR662 pKa = 11.84VAVTLASGAVEE673 pKa = 4.2TVTAGANGQWSVMFHH688 pKa = 6.72SDD690 pKa = 4.08DD691 pKa = 4.7LGTTGSGTMGYY702 pKa = 9.85QIAATDD708 pKa = 3.8PVGNSATASGSFGYY722 pKa = 10.7DD723 pKa = 2.91LVAPEE728 pKa = 4.93SPDD731 pKa = 3.12IIAFTRR737 pKa = 11.84DD738 pKa = 3.03AGSLLGIRR746 pKa = 11.84TDD748 pKa = 3.53LGEE751 pKa = 4.04NTYY754 pKa = 10.76EE755 pKa = 3.96IAAVDD760 pKa = 3.19AAGRR764 pKa = 11.84VTGVPNDD771 pKa = 3.84ASVSTHH777 pKa = 5.74GGYY780 pKa = 8.1GTYY783 pKa = 10.62DD784 pKa = 4.24FYY786 pKa = 11.99SSVPNGSYY794 pKa = 10.65LVVSDD799 pKa = 4.79HH800 pKa = 6.74DD801 pKa = 3.89TAGNEE806 pKa = 4.0TSTLLVVDD814 pKa = 3.68NTSAVTVDD822 pKa = 4.12LARR825 pKa = 11.84SGLGDD830 pKa = 3.83FDD832 pKa = 5.64FGTIDD837 pKa = 3.47LTFAPEE843 pKa = 3.78AQLTITEE850 pKa = 4.21AQLVALTGPDD860 pKa = 3.35HH861 pKa = 7.0ALVIQGDD868 pKa = 4.28SADD871 pKa = 3.76HH872 pKa = 5.09VTALGAEE879 pKa = 4.22DD880 pKa = 4.16SGQNTVIGGHH890 pKa = 5.67TYY892 pKa = 10.59SVYY895 pKa = 10.76TLGDD899 pKa = 3.87DD900 pKa = 3.97GASLIIDD907 pKa = 3.84DD908 pKa = 5.07QITHH912 pKa = 6.02LTII915 pKa = 4.16

Molecular weight: 91.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4JK15|A0A2T4JK15_9RHOB DUF3429 domain-containing protein OS=Rhodobacter veldkampii DSM 11550 OX=1185920 GN=C5F46_05720 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.12GGRR28 pKa = 11.84RR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.0GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3115

0

3115

961833

28

2468

308.8

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.249 ± 0.084

0.857 ± 0.014

5.696 ± 0.032

5.571 ± 0.043

3.509 ± 0.026

8.877 ± 0.046

1.999 ± 0.021

4.742 ± 0.033

2.744 ± 0.041

10.447 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.019

2.184 ± 0.02

5.494 ± 0.036

3.021 ± 0.021

7.6 ± 0.043

4.446 ± 0.027

5.243 ± 0.033

7.269 ± 0.036

1.437 ± 0.022

2.005 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski