Thalassotalea euphylliae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Thalassotalea

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3E0U2V0|A0A3E0U2V0_9GAMM Antitoxin OS=Thalassotalea euphylliae OX=1655234 GN=DXX94_11645 PE=3 SV=1
MM1 pKa = 7.3KK2 pKa = 10.65NKK4 pKa = 8.42VTFPLNCIALILASQLTGCGSDD26 pKa = 4.14GDD28 pKa = 4.12NTPQIDD34 pKa = 5.33DD35 pKa = 4.0SATDD39 pKa = 3.44NTPPVITLIGNEE51 pKa = 4.45SIDD54 pKa = 3.59VVYY57 pKa = 10.98NAVFGDD63 pKa = 4.4LGAEE67 pKa = 4.03AHH69 pKa = 7.12DD70 pKa = 4.2EE71 pKa = 4.11TDD73 pKa = 3.12GAITVTSQGTIDD85 pKa = 3.25TAMPGTYY92 pKa = 9.89EE93 pKa = 3.92LTYY96 pKa = 9.98TATDD100 pKa = 3.24ASGNSSQATRR110 pKa = 11.84TVVVLEE116 pKa = 4.63DD117 pKa = 3.71TSQSATITTPQAPFSLFYY135 pKa = 11.27DD136 pKa = 4.54GVLKK140 pKa = 10.85GADD143 pKa = 3.48YY144 pKa = 10.12WSEE147 pKa = 3.93EE148 pKa = 3.95PQILSAGMGFDD159 pKa = 4.43GIIGIDD165 pKa = 3.61APEE168 pKa = 3.98LTFDD172 pKa = 3.96SVLEE176 pKa = 4.26AGGAWSSDD184 pKa = 3.38INCGTGAQNYY194 pKa = 7.16TSTSTLDD201 pKa = 3.58QIAGAYY207 pKa = 9.17INQTPYY213 pKa = 11.62GEE215 pKa = 4.76LKK217 pKa = 10.83DD218 pKa = 3.91GAIGLDD224 pKa = 3.42GLPIVLSWPIDD235 pKa = 3.52TSTLSLTDD243 pKa = 3.67FQFTLNTGDD252 pKa = 3.13IVRR255 pKa = 11.84PLAIGPLPNFEE266 pKa = 4.76DD267 pKa = 3.88NEE269 pKa = 4.51RR270 pKa = 11.84NTPVAFGEE278 pKa = 4.24FGNRR282 pKa = 11.84LPSDD286 pKa = 3.65HH287 pKa = 7.63PDD289 pKa = 2.61ARR291 pKa = 11.84FPIKK295 pKa = 10.78LEE297 pKa = 3.79IVEE300 pKa = 5.24DD301 pKa = 4.07DD302 pKa = 4.1TPLMMVGPGGQVVSAVGLTWEE323 pKa = 4.79TDD325 pKa = 3.06SSPYY329 pKa = 10.52DD330 pKa = 3.71EE331 pKa = 5.54NNGPRR336 pKa = 11.84LVGAKK341 pKa = 9.94LNRR344 pKa = 11.84IEE346 pKa = 4.09GQMDD350 pKa = 3.9GEE352 pKa = 5.08GISTPIPLIPANDD365 pKa = 3.37ATVLYY370 pKa = 10.79DD371 pKa = 3.94EE372 pKa = 5.2GDD374 pKa = 3.17FMLRR378 pKa = 11.84MLTTGGFSPDD388 pKa = 3.4GVSGLTPNDD397 pKa = 3.17FEE399 pKa = 5.16RR400 pKa = 11.84FFRR403 pKa = 11.84IHH405 pKa = 6.9ANGVDD410 pKa = 3.69GSTVIIDD417 pKa = 3.81KK418 pKa = 10.97VGEE421 pKa = 4.07EE422 pKa = 4.44YY423 pKa = 10.77AVQGGTLRR431 pKa = 11.84VVGMSDD437 pKa = 3.35LGQPEE442 pKa = 4.15GGEE445 pKa = 4.42VVFDD449 pKa = 3.64ACYY452 pKa = 10.83SEE454 pKa = 6.09DD455 pKa = 3.62RR456 pKa = 11.84DD457 pKa = 3.83NYY459 pKa = 10.24IDD461 pKa = 4.11IIMVGDD467 pKa = 3.75EE468 pKa = 3.66EE469 pKa = 4.19AARR472 pKa = 11.84NITILEE478 pKa = 4.16IPSLEE483 pKa = 4.03EE484 pKa = 4.56GYY486 pKa = 10.55SAFYY490 pKa = 10.93NPGGPGSTPFEE501 pKa = 4.29GVSYY505 pKa = 7.74TAPGPRR511 pKa = 11.84DD512 pKa = 3.86LEE514 pKa = 4.16PVIIALDD521 pKa = 3.5DD522 pKa = 3.99PMRR525 pKa = 11.84VTYY528 pKa = 10.7VPGSEE533 pKa = 4.37NVEE536 pKa = 4.26TPDD539 pKa = 3.9NLMTFLFEE547 pKa = 4.75GIEE550 pKa = 4.12RR551 pKa = 11.84EE552 pKa = 4.1YY553 pKa = 10.75LLNMSSNYY561 pKa = 9.98TGDD564 pKa = 3.53TPVPLLFDD572 pKa = 3.37FHH574 pKa = 7.51GLNGTAEE581 pKa = 4.11QQNTDD586 pKa = 3.08SQFNQLAEE594 pKa = 4.24TEE596 pKa = 4.16NFILVTPKK604 pKa = 10.76AIGGWNVTGFPLGGDD619 pKa = 3.52ANDD622 pKa = 4.62LGFIDD627 pKa = 5.05ALINEE632 pKa = 5.25LSTTYY637 pKa = 10.84NIDD640 pKa = 3.22TNRR643 pKa = 11.84IYY645 pKa = 10.99AAGFSLGGFFSFEE658 pKa = 4.08LACQYY663 pKa = 11.38SDD665 pKa = 3.16TFAAIAPVSGVMTPNMANDD684 pKa = 4.38CLPEE688 pKa = 4.68RR689 pKa = 11.84PIPILQTHH697 pKa = 5.99GTADD701 pKa = 3.56DD702 pKa = 3.78QLPYY706 pKa = 10.92AQAQTVLQWWIDD718 pKa = 3.57FNQTDD723 pKa = 4.37VEE725 pKa = 4.13PAITDD730 pKa = 3.97LEE732 pKa = 4.45DD733 pKa = 3.38TFPDD737 pKa = 3.15NGTTVQRR744 pKa = 11.84YY745 pKa = 8.35VYY747 pKa = 10.86GNGTNGVSVEE757 pKa = 3.91HH758 pKa = 6.3LRR760 pKa = 11.84IEE762 pKa = 4.74GGQHH766 pKa = 5.6IWPGSAGDD774 pKa = 3.78SDD776 pKa = 4.21INMAEE781 pKa = 4.41EE782 pKa = 3.58IWSFFSNYY790 pKa = 10.0DD791 pKa = 3.75LNGKK795 pKa = 9.15IPDD798 pKa = 3.48

Molecular weight:
85.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3E0TII7|A0A3E0TII7_9GAMM Carboxy-S-adenosyl-L-methionine synthase OS=Thalassotalea euphylliae OX=1655234 GN=cmoA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.49GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight:
5.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3642

0

3642

1233835

38

8735

338.8

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.219 ± 0.039

0.927 ± 0.014

5.566 ± 0.042

6.145 ± 0.039

4.199 ± 0.027

6.506 ± 0.052

2.211 ± 0.021

6.364 ± 0.029

5.347 ± 0.053

10.234 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.024

4.636 ± 0.038

3.719 ± 0.025

5.166 ± 0.04

4.143 ± 0.032

6.727 ± 0.04

5.464 ± 0.04

6.9 ± 0.031

1.142 ± 0.014

3.056 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski