Microbacterium phage Rasovi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Goodmanvirus; unclassified Goodmanvirus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SWS5|A0A6M3SWS5_9CAUD Uncharacterized protein OS=Microbacterium phage Rasovi OX=2725621 GN=32 PE=4 SV=1
MM1 pKa = 7.6SYY3 pKa = 9.43ATVALMRR10 pKa = 11.84RR11 pKa = 11.84DD12 pKa = 3.24YY13 pKa = 11.76DD14 pKa = 3.61LANRR18 pKa = 11.84VAACVAVEE26 pKa = 4.94GISDD30 pKa = 4.66YY31 pKa = 10.96PEE33 pKa = 3.3QWAGEE38 pKa = 4.24NAWALAAQPGWAEE51 pKa = 3.72AWEE54 pKa = 4.5SAVVSHH60 pKa = 7.41PDD62 pKa = 3.12PDD64 pKa = 3.62YY65 pKa = 11.35RR66 pKa = 11.84PALDD70 pKa = 3.98EE71 pKa = 4.55GVITDD76 pKa = 4.02GMILSAVQSLHH87 pKa = 6.25AVPPVEE93 pKa = 3.9EE94 pKa = 4.29SAVVEE99 pKa = 4.18SDD101 pKa = 3.48PEE103 pKa = 4.17EE104 pKa = 4.1VV105 pKa = 3.26

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SWP6|A0A6M3SWP6_9CAUD Uncharacterized protein OS=Microbacterium phage Rasovi OX=2725621 GN=2 PE=4 SV=1
MM1 pKa = 6.97TRR3 pKa = 11.84GRR5 pKa = 11.84SRR7 pKa = 11.84GRR9 pKa = 11.84RR10 pKa = 11.84VSISVLTQYY19 pKa = 10.69PWTSSWEE26 pKa = 3.84AGIWDD31 pKa = 3.64YY32 pKa = 11.53HH33 pKa = 5.35GTLTTVVPDD42 pKa = 3.04IWTGTEE48 pKa = 3.94SVRR51 pKa = 11.84KK52 pKa = 9.36FDD54 pKa = 3.59VAFVTMPGAYY64 pKa = 9.46KK65 pKa = 10.85AMRR68 pKa = 11.84VWNEE72 pKa = 3.45RR73 pKa = 11.84PPWRR77 pKa = 11.84DD78 pKa = 2.58

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13720

41

976

217.8

23.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.853 ± 0.287

0.576 ± 0.12

6.399 ± 0.203

6.64 ± 0.332

2.959 ± 0.177

8.819 ± 0.306

1.691 ± 0.142

4.337 ± 0.241

4.534 ± 0.353

7.624 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.112

3.338 ± 0.219

5.736 ± 0.366

3.95 ± 0.17

5.904 ± 0.368

5.386 ± 0.303

6.669 ± 0.284

7.194 ± 0.292

2.478 ± 0.203

2.784 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski