Proteome-pI 2.0 - Proteome Isoelectric Point Database

Candidatus Pantoea carbekii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Pantoea

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|U3U2N1|U3U2N1_9GAMM Uracil-DNA glycosylase OS=Candidatus Pantoea carbekii OX=1235990 GN=ung PE=3 SV=1

MM1 pKa = 7.27VHH3 pKa = 7.37DD4 pKa = 4.17SQMDD8 pKa = 3.58LFDD11 pKa = 3.02IHH13 pKa = 6.63TGYY16 pKa = 11.12SSGIYY21 pKa = 10.0NLNQRR26 pKa = 11.84AWAEE30 pKa = 3.83QLYY33 pKa = 10.46LAICNYY39 pKa = 9.38IQQNLDD45 pKa = 3.22PSNRR49 pKa = 11.84AHH51 pKa = 6.8HH52 pKa = 5.92PVIDD56 pKa = 3.81KK57 pKa = 10.36LQEE60 pKa = 3.63RR61 pKa = 11.84MADD64 pKa = 3.36KK65 pKa = 10.52IYY67 pKa = 11.32VNFSLFQSMPDD78 pKa = 2.73AWGINQLFPVFPLEE92 pKa = 5.07GLNQTLKK99 pKa = 10.61RR100 pKa = 11.84RR101 pKa = 11.84AVLLDD106 pKa = 3.3ITCDD110 pKa = 3.06SDD112 pKa = 5.5GIIDD116 pKa = 4.49HH117 pKa = 6.94YY118 pKa = 11.27IDD120 pKa = 4.5GDD122 pKa = 4.36GIATTMPIADD132 pKa = 3.97YY133 pKa = 10.83DD134 pKa = 3.97INNPPLLGFFMAGAYY149 pKa = 9.43QKK151 pKa = 10.76ILGNMHH157 pKa = 6.7NLFGDD162 pKa = 4.22TEE164 pKa = 4.32AVNVFVFSNGNIEE177 pKa = 4.27IQLSDD182 pKa = 3.7QGDD185 pKa = 3.92TVADD189 pKa = 3.48MLQYY193 pKa = 10.67VQLNAADD200 pKa = 4.19LLII203 pKa = 5.01

Molecular weight: 22.86 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|U3U8X0|U3U8X0_9GAMM 2-oxoglutarate reductase OS=Candidatus Pantoea carbekii OX=1235990 GN=serA PE=3 SV=1

MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.32RR12 pKa = 11.84MRR14 pKa = 11.84SHH16 pKa = 7.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.06HH26 pKa = 5.06GRR28 pKa = 11.84QIISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84LTVSSKK47 pKa = 10.12NN48 pKa = 3.47

Molecular weight: 5.75 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
825	0	825	260910	45	1413	316.3	35.72

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.593 ± 0.076	1.455 ± 0.033	4.548 ± 0.057	5.321 ± 0.075	4.18 ± 0.056	6.152 ± 0.061	2.528 ± 0.035	9.517 ± 0.077	6.465 ± 0.082	10.598 ± 0.085

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.623 ± 0.037	5.64 ± 0.066	3.48 ± 0.037	4.035 ± 0.043	5.042 ± 0.05	6.216 ± 0.052	5.114 ± 0.045	5.981 ± 0.061	1.107 ± 0.033	3.405 ± 0.05

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski