Microbacterium hydrocarbonoxydans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae;

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2HQV1|A0A0M2HQV1_9MICO Gluconate 5-dehydrogenase OS=Microbacterium hydrocarbonoxydans OX=273678 GN=gno_2 PE=4 SV=1
MM1 pKa = 7.18GVVGRR6 pKa = 11.84DD7 pKa = 3.15RR8 pKa = 11.84AAEE11 pKa = 4.0PAEE14 pKa = 4.11GVYY17 pKa = 10.19SICYY21 pKa = 9.54VNGFQTQPGEE31 pKa = 4.18LEE33 pKa = 3.97SWPDD37 pKa = 3.34DD38 pKa = 3.78VLLHH42 pKa = 7.27RR43 pKa = 11.84DD44 pKa = 3.64GDD46 pKa = 4.19PLFDD50 pKa = 5.63PDD52 pKa = 4.11WPDD55 pKa = 3.05EE56 pKa = 4.12ALLDD60 pKa = 3.8TSTGDD65 pKa = 3.13RR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 3.87RR69 pKa = 11.84IAATIEE75 pKa = 3.57PWIIGCADD83 pKa = 3.23AGFDD87 pKa = 4.38AVEE90 pKa = 3.97FDD92 pKa = 4.34NLDD95 pKa = 3.25SYY97 pKa = 10.78TRR99 pKa = 11.84SEE101 pKa = 4.38GALSLDD107 pKa = 4.66DD108 pKa = 4.9NLDD111 pKa = 3.41LATRR115 pKa = 11.84LVEE118 pKa = 4.12IAHH121 pKa = 6.21GAGLAAGQKK130 pKa = 9.45NAAEE134 pKa = 4.4DD135 pKa = 3.49AAALHH140 pKa = 6.09AQAGFDD146 pKa = 3.46FAVTEE151 pKa = 4.11EE152 pKa = 4.1CAAYY156 pKa = 9.54QEE158 pKa = 4.54CPVYY162 pKa = 10.55IEE164 pKa = 4.67VYY166 pKa = 8.39GAHH169 pKa = 6.58VIDD172 pKa = 4.24IEE174 pKa = 4.54YY175 pKa = 10.72VDD177 pKa = 3.7EE178 pKa = 4.56LPRR181 pKa = 11.84TFAEE185 pKa = 4.28MCADD189 pKa = 4.06PDD191 pKa = 4.09SPASMVLRR199 pKa = 11.84DD200 pKa = 4.36RR201 pKa = 11.84EE202 pKa = 4.42LLTPDD207 pKa = 3.49DD208 pKa = 3.78EE209 pKa = 6.49GYY211 pKa = 11.3AFEE214 pKa = 4.46TCC216 pKa = 4.54

Molecular weight: 23.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2HRE8|A0A0M2HRE8_9MICO Mannitol-1-phosphate 5-dehydrogenase OS=Microbacterium hydrocarbonoxydans OX=273678 GN=mtlD PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3663

0

3663

1217945

31

2582

332.5

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.597 ± 0.067

0.476 ± 0.009

6.346 ± 0.041

5.717 ± 0.038

3.184 ± 0.025

8.93 ± 0.055

1.944 ± 0.021

4.803 ± 0.029

2.012 ± 0.03

9.918 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.839 ± 0.019

1.986 ± 0.022

5.282 ± 0.033

2.832 ± 0.022

7.0 ± 0.051

5.809 ± 0.031

6.069 ± 0.041

8.686 ± 0.039

1.568 ± 0.018

2.002 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski