Hubei myriapoda virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJG9|A0A1L3KJG9_9VIRU Uncharacterized protein OS=Hubei myriapoda virus 3 OX=1922932 PE=4 SV=1
MM1 pKa = 7.33SKK3 pKa = 8.4EE4 pKa = 4.23TKK6 pKa = 10.59AEE8 pKa = 3.85AVPEE12 pKa = 4.24NNPSLAFTNEE22 pKa = 4.06DD23 pKa = 2.98VSLPTSHH30 pKa = 7.22SAFTEE35 pKa = 4.53PGTSPSTVARR45 pKa = 11.84DD46 pKa = 3.45IGCTGVRR53 pKa = 11.84VFPEE57 pKa = 4.91LYY59 pKa = 10.27EE60 pKa = 4.7KK61 pKa = 10.46DD62 pKa = 3.87YY63 pKa = 11.36QIDD66 pKa = 4.12SITWDD71 pKa = 3.04IGKK74 pKa = 9.72SPGTILKK81 pKa = 9.97VFTISPDD88 pKa = 3.14MHH90 pKa = 6.29PQIAYY95 pKa = 9.68LMKK98 pKa = 9.99MFQFWSGTIDD108 pKa = 3.05IVFKK112 pKa = 10.84IAGTGFNAGLIGITTANPLVPLEE135 pKa = 4.39TYY137 pKa = 11.11KK138 pKa = 10.05NTTDD142 pKa = 3.68FCNFDD147 pKa = 3.37TQYY150 pKa = 11.93ADD152 pKa = 3.7PKK154 pKa = 10.52QLALFKK160 pKa = 9.67VTPQDD165 pKa = 2.96FHH167 pKa = 9.14IYY169 pKa = 7.71NTHH172 pKa = 6.3INSINQTATGDD183 pKa = 3.41TDD185 pKa = 3.47ADD187 pKa = 4.37KK188 pKa = 10.05ITKK191 pKa = 10.33ASGLAAKK198 pKa = 10.14VAVFVSLQLASGTSGNTSVDD218 pKa = 2.58IGVFLVASKK227 pKa = 10.58SFRR230 pKa = 11.84LHH232 pKa = 6.27QILPAPSSITNNISFEE248 pKa = 4.41LLNHH252 pKa = 6.57ALDD255 pKa = 3.58VSNPYY260 pKa = 9.69TKK262 pKa = 9.9TATLEE267 pKa = 3.87RR268 pKa = 11.84CRR270 pKa = 11.84GMMAVPLSLLDD281 pKa = 3.45KK282 pKa = 10.01PKK284 pKa = 10.9RR285 pKa = 11.84FIANCAGATGWEE297 pKa = 3.83NDD299 pKa = 2.85RR300 pKa = 11.84RR301 pKa = 11.84IWNKK305 pKa = 6.52THH307 pKa = 7.11TYY309 pKa = 10.77NSINGPIVIEE319 pKa = 4.16NVTYY323 pKa = 10.17DD324 pKa = 3.5YY325 pKa = 11.06PAFLGFYY332 pKa = 8.81EE333 pKa = 4.27SHH335 pKa = 6.73PTTVVSIPVNQNDD348 pKa = 3.48EE349 pKa = 4.5TALSSHH355 pKa = 6.17VATLDD360 pKa = 3.27YY361 pKa = 10.7TDD363 pKa = 4.1YY364 pKa = 11.24FEE366 pKa = 4.5EE367 pKa = 5.15HH368 pKa = 6.96PEE370 pKa = 3.97EE371 pKa = 4.68KK372 pKa = 10.3FVGVGFYY379 pKa = 10.05TKK381 pKa = 10.69NCIPASLIPRR391 pKa = 11.84KK392 pKa = 9.79SVLQNIRR399 pKa = 11.84TVEE402 pKa = 4.06SQNLDD407 pKa = 3.35GALGLYY413 pKa = 10.57AIDD416 pKa = 4.99NPEE419 pKa = 3.98EE420 pKa = 4.12YY421 pKa = 10.56QNLTSMLPQHH431 pKa = 6.48PRR433 pKa = 11.84FDD435 pKa = 4.23SIRR438 pKa = 11.84NSFQWGLAMINPTTKK453 pKa = 10.27HH454 pKa = 4.56YY455 pKa = 9.67TNLHH459 pKa = 5.68SFTTLKK465 pKa = 10.81NLTCTPPDD473 pKa = 3.42WEE475 pKa = 5.31LDD477 pKa = 3.76FSLKK481 pKa = 10.49GPSSGEE487 pKa = 3.95SFLLFTDD494 pKa = 4.28TYY496 pKa = 10.91DD497 pKa = 3.48YY498 pKa = 11.25QAADD502 pKa = 5.29LISIDD507 pKa = 3.08IGLPSFQTEE516 pKa = 3.91HH517 pKa = 6.7LASYY521 pKa = 8.78MYY523 pKa = 10.6NKK525 pKa = 9.56PIQSNQSVLLSMVDD539 pKa = 3.62TIDD542 pKa = 4.71DD543 pKa = 4.7KK544 pKa = 11.32IVSQAKK550 pKa = 9.87LYY552 pKa = 9.05PEE554 pKa = 5.25GYY556 pKa = 7.69ITVPTVSQLTFYY568 pKa = 11.22GIDD571 pKa = 3.46RR572 pKa = 11.84YY573 pKa = 11.04KK574 pKa = 11.01LIAQRR579 pKa = 11.84VVPRR583 pKa = 11.84NYY585 pKa = 10.3EE586 pKa = 3.74MRR588 pKa = 11.84PTAVQNLVTNMIAARR603 pKa = 11.84TQQEE607 pKa = 4.31SQTTYY612 pKa = 6.43QTKK615 pKa = 8.43YY616 pKa = 9.34TNFLLSQHH624 pKa = 6.5KK625 pKa = 10.53LLTKK629 pKa = 10.23KK630 pKa = 10.65FKK632 pKa = 10.35TVDD635 pKa = 3.4KK636 pKa = 10.59LNKK639 pKa = 10.12YY640 pKa = 10.32LDD642 pKa = 3.58EE643 pKa = 5.87HH644 pKa = 5.05STKK647 pKa = 9.93WPAEE651 pKa = 3.98PLPPEE656 pKa = 4.31QPSAPDD662 pKa = 3.13SS663 pKa = 3.75

Molecular weight: 74.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJL3|A0A1L3KJL3_9VIRU Uncharacterized protein OS=Hubei myriapoda virus 3 OX=1922932 PE=4 SV=1
MM1 pKa = 7.62PFLYY5 pKa = 10.24KK6 pKa = 9.9IEE8 pKa = 4.47SLIQLLDD15 pKa = 4.36FIEE18 pKa = 5.12DD19 pKa = 3.42QFQVDD24 pKa = 4.53FEE26 pKa = 5.04LGNSIYY32 pKa = 11.01YY33 pKa = 10.6LLQFPKK39 pKa = 9.78PWKK42 pKa = 9.26PHH44 pKa = 4.85TNEE47 pKa = 3.38PWFLYY52 pKa = 10.31YY53 pKa = 10.51CDD55 pKa = 3.7IYY57 pKa = 11.32EE58 pKa = 4.19HH59 pKa = 6.65FKK61 pKa = 10.32RR62 pKa = 11.84TIEE65 pKa = 4.09NDD67 pKa = 3.59PQHH70 pKa = 6.44LHH72 pKa = 7.15PDD74 pKa = 3.53LGVTIDD80 pKa = 4.33FLPDD84 pKa = 3.38PEE86 pKa = 4.71SFVAGATNSKK96 pKa = 10.41LFVYY100 pKa = 10.0KK101 pKa = 9.97YY102 pKa = 10.52QKK104 pKa = 10.53NPRR107 pKa = 11.84KK108 pKa = 8.88KK109 pKa = 8.72TNLLAHH115 pKa = 6.61TLNCVCEE122 pKa = 3.98DD123 pKa = 3.68CKK125 pKa = 11.21YY126 pKa = 10.97SEE128 pKa = 4.19QLALALDD135 pKa = 3.87RR136 pKa = 11.84KK137 pKa = 10.39DD138 pKa = 3.91RR139 pKa = 11.84LVSAIKK145 pKa = 10.44RR146 pKa = 11.84KK147 pKa = 8.04TDD149 pKa = 3.16HH150 pKa = 6.85NGQLLQRR157 pKa = 11.84PKK159 pKa = 10.59FGQKK163 pKa = 10.18KK164 pKa = 6.95IQNYY168 pKa = 8.13IRR170 pKa = 11.84NSGSEE175 pKa = 3.9DD176 pKa = 3.6LFRR179 pKa = 11.84QHH181 pKa = 6.81TSGCSANQLYY191 pKa = 10.85SSLAKK196 pKa = 8.57YY197 pKa = 7.31TKK199 pKa = 8.48PTVEE203 pKa = 3.86ITSPTRR209 pKa = 11.84DD210 pKa = 3.23MLDD213 pKa = 3.31FRR215 pKa = 11.84FKK217 pKa = 10.15EE218 pKa = 4.07VQRR221 pKa = 11.84KK222 pKa = 9.1KK223 pKa = 9.8EE224 pKa = 4.12TLSDD228 pKa = 3.61EE229 pKa = 5.3LISKK233 pKa = 9.25LAIALTSTGLPDD245 pKa = 5.91AIPKK249 pKa = 9.85ILRR252 pKa = 11.84QLKK255 pKa = 9.46VPEE258 pKa = 4.26GKK260 pKa = 9.06IDD262 pKa = 3.38QLAYY266 pKa = 10.61DD267 pKa = 4.36NQTVPPDD274 pKa = 3.49VTPKK278 pKa = 10.89NKK280 pKa = 8.67TQSLPLL286 pKa = 4.39

Molecular weight: 33.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3510

286

2242

877.5

100.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.014 ± 0.761

1.51 ± 0.403

6.296 ± 0.326

5.755 ± 0.451

5.726 ± 0.707

4.53 ± 0.483

2.735 ± 0.12

6.838 ± 0.522

7.664 ± 0.803

9.516 ± 0.636

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.909 ± 0.196

5.641 ± 0.302

5.071 ± 1.01

4.957 ± 0.3

3.903 ± 0.472

6.781 ± 0.884

6.581 ± 1.083

4.815 ± 0.272

0.969 ± 0.044

3.789 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski