Klebsiella phage 1 LV-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2FG38|A0A2Z2FG38_9CAUD Head-tail connector protein OS=Klebsiella phage 1 LV-2017 OX=1960654 PE=4 SV=1
MM1 pKa = 7.02STTIEE6 pKa = 4.13MLRR9 pKa = 11.84AQCRR13 pKa = 11.84IDD15 pKa = 4.35IDD17 pKa = 3.89DD18 pKa = 3.78TTEE21 pKa = 4.6DD22 pKa = 3.75EE23 pKa = 5.14VLTLYY28 pKa = 10.85YY29 pKa = 10.61GAARR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 10.06AEE37 pKa = 3.91NFINRR42 pKa = 11.84HH43 pKa = 4.95LYY45 pKa = 9.59EE46 pKa = 4.36EE47 pKa = 4.77EE48 pKa = 4.25VPEE51 pKa = 4.23TDD53 pKa = 3.79PDD55 pKa = 4.05GLVIADD61 pKa = 5.31DD62 pKa = 3.6ILLALMLLVGHH73 pKa = 7.03WYY75 pKa = 10.03EE76 pKa = 4.08NRR78 pKa = 11.84EE79 pKa = 4.05EE80 pKa = 4.27SSDD83 pKa = 3.56AAKK86 pKa = 10.17TSIPFGFTSLIEE98 pKa = 4.07PYY100 pKa = 10.18RR101 pKa = 11.84YY102 pKa = 9.5IPLL105 pKa = 4.15

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2FBK9|A0A2Z2FBK9_9CAUD Tail length tape-measure protein 1 OS=Klebsiella phage 1 LV-2017 OX=1960654 PE=4 SV=1
MM1 pKa = 7.27NQTQFQKK8 pKa = 11.07AAGISAGLAARR19 pKa = 11.84WFPHH23 pKa = 4.91ITAAMKK29 pKa = 10.48EE30 pKa = 4.1FGITAPLDD38 pKa = 3.27QAMFIAQMGHH48 pKa = 5.67EE49 pKa = 4.41SGGFTRR55 pKa = 11.84LVEE58 pKa = 4.11NLNYY62 pKa = 10.58SADD65 pKa = 3.74SLVPTFGKK73 pKa = 9.97HH74 pKa = 6.76RR75 pKa = 11.84ITAQQAAALGRR86 pKa = 11.84TATQPANQRR95 pKa = 11.84AIANLVYY102 pKa = 10.5GGEE105 pKa = 4.03WGKK108 pKa = 11.39KK109 pKa = 8.57NLGNQVAGDD118 pKa = 3.34GWKK121 pKa = 10.36YY122 pKa = 10.29RR123 pKa = 11.84GRR125 pKa = 11.84GLKK128 pKa = 9.78QVTGLSNYY136 pKa = 9.44RR137 pKa = 11.84SCGLALKK144 pKa = 10.57LEE146 pKa = 4.69LVTQPEE152 pKa = 4.28LLEE155 pKa = 5.57RR156 pKa = 11.84DD157 pKa = 3.68DD158 pKa = 3.83YY159 pKa = 11.51AARR162 pKa = 11.84SAAWFYY168 pKa = 11.49VSHH171 pKa = 6.75GCLLHH176 pKa = 7.67SGDD179 pKa = 3.88VEE181 pKa = 4.67RR182 pKa = 11.84VTLLINGGRR191 pKa = 11.84NGLDD195 pKa = 3.01KK196 pKa = 10.91RR197 pKa = 11.84RR198 pKa = 11.84ALFNLAKK205 pKa = 10.38SVLAA209 pKa = 4.81

Molecular weight: 22.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

6285

55

1128

261.9

29.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.469 ± 0.69

0.875 ± 0.206

5.664 ± 0.467

6.778 ± 0.432

3.628 ± 0.317

7.192 ± 0.363

1.623 ± 0.287

4.821 ± 0.222

5.442 ± 0.363

9.037 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.195

4.36 ± 0.334

3.882 ± 0.457

4.041 ± 0.606

6.364 ± 0.311

6.794 ± 0.834

5.664 ± 0.398

6.317 ± 0.448

1.655 ± 0.238

2.689 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski