Paraflavitalea soli

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Paraflavitalea

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B7MUW5|A0A3B7MUW5_9BACT RagB/SusD family nutrient uptake outer membrane protein OS=Paraflavitalea soli OX=2315862 GN=D3H65_23970 PE=3 SV=1
MM1 pKa = 7.26YY2 pKa = 10.19SYY4 pKa = 11.81ALLEE8 pKa = 4.33SGCYY12 pKa = 9.72YY13 pKa = 10.71LIQEE17 pKa = 4.55KK18 pKa = 10.61EE19 pKa = 4.05EE20 pKa = 4.64SPVSLIKK27 pKa = 10.29VTVDD31 pKa = 2.67TDD33 pKa = 3.51YY34 pKa = 11.56CLYY37 pKa = 7.68VTNYY41 pKa = 10.84AEE43 pKa = 4.48VPVLEE48 pKa = 4.73WKK50 pKa = 10.6KK51 pKa = 9.69KK52 pKa = 7.38TDD54 pKa = 4.36PIFDD58 pKa = 3.77ILEE61 pKa = 4.3LLSDD65 pKa = 4.21DD66 pKa = 5.31KK67 pKa = 11.57VKK69 pKa = 10.36EE70 pKa = 4.1WEE72 pKa = 4.16SVYY75 pKa = 11.25NDD77 pKa = 3.51NQDD80 pKa = 2.96AYY82 pKa = 11.46YY83 pKa = 10.81EE84 pKa = 4.34EE85 pKa = 6.59DD86 pKa = 4.08DD87 pKa = 4.74DD88 pKa = 5.0

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B7MPW6|A0A3B7MPW6_9BACT Uncharacterized protein OS=Paraflavitalea soli OX=2315862 GN=D3H65_15810 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.56RR3 pKa = 11.84TFQPHH8 pKa = 4.54RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.38SVHH16 pKa = 5.05GFRR19 pKa = 11.84KK20 pKa = 10.11RR21 pKa = 11.84MQTANGRR28 pKa = 11.84KK29 pKa = 8.71VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.77KK41 pKa = 10.08LTVSDD46 pKa = 3.83EE47 pKa = 4.55RR48 pKa = 11.84KK49 pKa = 10.22LKK51 pKa = 10.73

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6406

0

6406

2480089

21

5738

387.2

43.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.087 ± 0.036

0.805 ± 0.008

5.143 ± 0.018

5.126 ± 0.033

4.643 ± 0.022

7.225 ± 0.03

1.928 ± 0.014

6.602 ± 0.022

6.188 ± 0.032

9.458 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.014

5.3 ± 0.032

4.226 ± 0.02

4.157 ± 0.021

4.434 ± 0.02

6.044 ± 0.024

6.242 ± 0.038

6.507 ± 0.022

1.431 ± 0.013

4.192 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski