Cohnella luojiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4577 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8LTM0|A0A4Y8LTM0_9BACL Extracellular solute-binding protein OS=Cohnella luojiensis OX=652876 GN=E2980_18270 PE=4 SV=1
MM1 pKa = 7.17LTITEE6 pKa = 4.27YY7 pKa = 10.94SVEE10 pKa = 4.02LVKK13 pKa = 11.09DD14 pKa = 3.68PFQILSGKK22 pKa = 10.16RR23 pKa = 11.84YY24 pKa = 9.99EE25 pKa = 4.48FLLDD29 pKa = 4.05LDD31 pKa = 4.9LEE33 pKa = 4.72EE34 pKa = 7.05DD35 pKa = 4.13DD36 pKa = 5.21EE37 pKa = 5.32LYY39 pKa = 10.6SANGIAIKK47 pKa = 10.38AVYY50 pKa = 9.82KK51 pKa = 10.6VDD53 pKa = 4.42AEE55 pKa = 4.33QQGSIVNYY63 pKa = 10.55DD64 pKa = 4.59LIEE67 pKa = 4.63KK68 pKa = 8.89STGKK72 pKa = 10.57YY73 pKa = 10.19LDD75 pKa = 4.31LDD77 pKa = 3.79MEE79 pKa = 4.68AEE81 pKa = 4.12EE82 pKa = 4.37EE83 pKa = 3.89AALAAFCQEE92 pKa = 4.56HH93 pKa = 6.13YY94 pKa = 11.11SEE96 pKa = 5.49AEE98 pKa = 3.91EE99 pKa = 4.09

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8LWJ5|A0A4Y8LWJ5_9BACL MerR family transcriptional regulator OS=Cohnella luojiensis OX=652876 GN=E2980_11960 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.57NGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.61KK37 pKa = 10.35GRR39 pKa = 11.84KK40 pKa = 8.89VLTAA44 pKa = 4.27

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4577

0

4577

1446148

26

3037

316.0

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.123 ± 0.041

0.748 ± 0.01

5.25 ± 0.028

6.689 ± 0.042

4.204 ± 0.029

7.518 ± 0.034

2.035 ± 0.016

6.796 ± 0.034

5.347 ± 0.031

10.089 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.781 ± 0.017

3.882 ± 0.029

4.11 ± 0.021

3.587 ± 0.019

5.375 ± 0.037

6.441 ± 0.028

5.241 ± 0.033

7.084 ± 0.028

1.347 ± 0.017

3.352 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski