Mannheimia phage PHL101

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Baylorvirus; Mannheimia virus PHL101

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q19UX5|Q19UX5_9CAUD DksA C4-type domain-containing protein OS=Mannheimia phage PHL101 OX=2681596 PE=4 SV=1
MM1 pKa = 7.71IKK3 pKa = 9.76PDD5 pKa = 3.43RR6 pKa = 11.84LRR8 pKa = 11.84AVLTQTIDD16 pKa = 2.8IFQANPEE23 pKa = 4.18KK24 pKa = 10.97LILQYY29 pKa = 11.35DD30 pKa = 3.47KK31 pKa = 11.76GKK33 pKa = 10.27IKK35 pKa = 10.91SKK37 pKa = 10.46GSQSHH42 pKa = 5.1SFEE45 pKa = 3.76YY46 pKa = 10.28HH47 pKa = 6.04YY48 pKa = 11.04DD49 pKa = 3.55LEE51 pKa = 5.32LIVVDD56 pKa = 5.76FPYY59 pKa = 10.75HH60 pKa = 6.57PDD62 pKa = 3.2VLFVPVLNFVRR73 pKa = 11.84NEE75 pKa = 3.66QWEE78 pKa = 4.17LLQNPEE84 pKa = 4.35LQDD87 pKa = 3.83KK88 pKa = 10.98IEE90 pKa = 4.79FEE92 pKa = 4.06IDD94 pKa = 3.32HH95 pKa = 6.65NNHH98 pKa = 5.95EE99 pKa = 4.87SYY101 pKa = 10.64DD102 pKa = 3.47IYY104 pKa = 10.87IRR106 pKa = 11.84LPLTEE111 pKa = 3.78RR112 pKa = 11.84VIVKK116 pKa = 9.49EE117 pKa = 3.92QDD119 pKa = 2.86GHH121 pKa = 5.8LTATHH126 pKa = 6.88ADD128 pKa = 3.92EE129 pKa = 5.6PNLADD134 pKa = 3.28VSPFTRR140 pKa = 11.84LTEE143 pKa = 4.04YY144 pKa = 10.46EE145 pKa = 4.45VYY147 pKa = 10.83LKK149 pKa = 11.0DD150 pKa = 3.44EE151 pKa = 5.83LIYY154 pKa = 10.63QWAEE158 pKa = 3.59PNEE161 pKa = 4.11

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q19UU9|Q19UU9_9CAUD Uncharacterized protein OS=Mannheimia phage PHL101 OX=2681596 PE=4 SV=1
MM1 pKa = 7.6SKK3 pKa = 10.1QLYY6 pKa = 8.71YY7 pKa = 10.17RR8 pKa = 11.84HH9 pKa = 6.55KK10 pKa = 10.95SRR12 pKa = 11.84WNRR15 pKa = 11.84LNRR18 pKa = 11.84LKK20 pKa = 11.02KK21 pKa = 8.75MNKK24 pKa = 8.89RR25 pKa = 11.84RR26 pKa = 11.84LNLFLQEE33 pKa = 3.93KK34 pKa = 9.65RR35 pKa = 11.84VANLEE40 pKa = 3.9SRR42 pKa = 11.84LADD45 pKa = 3.5EE46 pKa = 5.37VEE48 pKa = 3.98LAKK51 pKa = 10.99LNSEE55 pKa = 4.42QIGDD59 pKa = 4.08LLQALEE65 pKa = 4.46LKK67 pKa = 10.51VNRR70 pKa = 11.84LIRR73 pKa = 11.84QNEE76 pKa = 3.99KK77 pKa = 10.51LKK79 pKa = 11.14ARR81 pKa = 11.84VIKK84 pKa = 10.54LEE86 pKa = 4.32KK87 pKa = 10.43VKK89 pKa = 9.1QTQKK93 pKa = 10.64QGLFAMFKK101 pKa = 10.28KK102 pKa = 10.91VLGSKK107 pKa = 10.44GNPP110 pKa = 3.22

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10274

58

970

209.7

23.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.855 ± 0.431

1.1 ± 0.209

5.1 ± 0.26

7.427 ± 0.396

4.078 ± 0.274

6.025 ± 0.382

1.703 ± 0.131

6.512 ± 0.309

7.903 ± 0.47

9.276 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.191

5.402 ± 0.381

3.231 ± 0.183

5.071 ± 0.275

5.091 ± 0.258

5.801 ± 0.251

5.655 ± 0.361

6.239 ± 0.336

1.275 ± 0.14

3.193 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski