Halomicronema hongdechloris C2206

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochlorotrichaceae; Halomicronema; Halomicronema hongdechloris

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z3HUC4|A0A1Z3HUC4_9CYAN Tetratricopeptide repeat domain protein OS=Halomicronema hongdechloris C2206 OX=1641165 GN=XM38_048670 PE=4 SV=1
MM1 pKa = 7.76SKK3 pKa = 10.72LLWKK7 pKa = 10.53SLLAAPAALGAVLAISGTSVAAEE30 pKa = 3.81SVEE33 pKa = 3.89PSFNEE38 pKa = 3.81LSAAEE43 pKa = 4.22PAQLAQVTSVDD54 pKa = 3.76EE55 pKa = 4.57LSDD58 pKa = 3.72VLPSDD63 pKa = 3.25WAYY66 pKa = 9.08TALQNLVEE74 pKa = 4.45TYY76 pKa = 10.83GCIEE80 pKa = 4.99GYY82 pKa = 9.35PNRR85 pKa = 11.84TFRR88 pKa = 11.84GDD90 pKa = 3.28RR91 pKa = 11.84ALTRR95 pKa = 11.84YY96 pKa = 8.99EE97 pKa = 3.9FAAGLNACLDD107 pKa = 4.09VISNLIVGGGVGEE120 pKa = 4.48SDD122 pKa = 3.47LATIRR127 pKa = 11.84RR128 pKa = 11.84LQEE131 pKa = 3.45EE132 pKa = 4.51FQAEE136 pKa = 4.36LATLRR141 pKa = 11.84GRR143 pKa = 11.84VDD145 pKa = 3.18ALEE148 pKa = 4.62ADD150 pKa = 4.06VAEE153 pKa = 4.67LEE155 pKa = 4.39ANQFSTTTKK164 pKa = 10.51LRR166 pKa = 11.84GQVDD170 pKa = 3.42SHH172 pKa = 7.2IVAPFDD178 pKa = 3.53EE179 pKa = 5.08LEE181 pKa = 4.21GVEE184 pKa = 5.52DD185 pKa = 3.57STTFTNRR192 pKa = 11.84VRR194 pKa = 11.84MNFDD198 pKa = 3.05TSFTGEE204 pKa = 3.53DD205 pKa = 3.22RR206 pKa = 11.84LRR208 pKa = 11.84VRR210 pKa = 11.84LQASGGADD218 pKa = 3.27PLVAGGGLANGDD230 pKa = 4.11DD231 pKa = 3.81ASGDD235 pKa = 2.95GDD237 pKa = 4.44YY238 pKa = 11.47NVDD241 pKa = 3.48IDD243 pKa = 3.97DD244 pKa = 4.87FYY246 pKa = 12.0YY247 pKa = 10.88LFPVGDD253 pKa = 3.89RR254 pKa = 11.84LDD256 pKa = 4.0IIIAANGIVTDD267 pKa = 4.63DD268 pKa = 4.0YY269 pKa = 11.55VVSTIVPFDD278 pKa = 4.0GPSVADD284 pKa = 3.48PGGPVFYY291 pKa = 10.35DD292 pKa = 3.59FDD294 pKa = 3.96MGGSAGAGFSFALTNNIAIDD314 pKa = 3.37AGYY317 pKa = 10.73SFDD320 pKa = 3.91EE321 pKa = 5.12AEE323 pKa = 4.63GADD326 pKa = 3.86PLIGISAASEE336 pKa = 3.6QSYY339 pKa = 10.15IGQVTFISDD348 pKa = 4.84GILDD352 pKa = 3.89LAGTFIRR359 pKa = 11.84GDD361 pKa = 3.59SGDD364 pKa = 3.74GAFTNTFAALANLDD378 pKa = 3.77FGRR381 pKa = 11.84FMIGGYY387 pKa = 10.1FSYY390 pKa = 10.8HH391 pKa = 6.87DD392 pKa = 4.8LDD394 pKa = 3.77GTPAVGDD401 pKa = 4.04DD402 pKa = 4.69DD403 pKa = 5.8FTTSWQAGIAVPDD416 pKa = 3.98LFIEE420 pKa = 5.37GAQLGAYY427 pKa = 7.36YY428 pKa = 9.55TALPEE433 pKa = 4.18YY434 pKa = 10.56ASGVNPYY441 pKa = 9.22MIEE444 pKa = 4.23GYY446 pKa = 10.73YY447 pKa = 10.12SIPVNQFLTITPALIYY463 pKa = 10.96GDD465 pKa = 4.17IDD467 pKa = 3.45SGAADD472 pKa = 3.42EE473 pKa = 4.29EE474 pKa = 4.48AFYY477 pKa = 10.98GAIRR481 pKa = 11.84ATFEE485 pKa = 3.85FF486 pKa = 4.77

Molecular weight: 51.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z3HGK3|A0A1Z3HGK3_9CYAN Uncharacterized protein OS=Halomicronema hongdechloris C2206 OX=1641165 GN=XM38_003020 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 8.94RR4 pKa = 11.84TLGGTVRR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.12RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TQTGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.54GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.42

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5211

0

5211

1532136

29

3109

294.0

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.726 ± 0.038

1.019 ± 0.011

5.417 ± 0.03

5.6 ± 0.036

3.489 ± 0.021

7.101 ± 0.035

2.292 ± 0.02

5.296 ± 0.027

2.784 ± 0.029

11.66 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.017

2.91 ± 0.023

5.553 ± 0.03

5.946 ± 0.036

6.375 ± 0.03

5.967 ± 0.026

5.626 ± 0.023

6.738 ± 0.028

1.679 ± 0.016

2.866 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski