Alteromonadaceae bacterium Bs31

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; unclassified Alteromonadaceae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4297 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y6CG80|A0A1Y6CG80_9ALTE DNA topoisomerase 4 subunit B OS=Alteromonadaceae bacterium Bs31 OX=1304903 GN=parE PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 9.98PLAQKK7 pKa = 10.04IHH9 pKa = 6.45LALLTALLLGGCGGDD24 pKa = 3.38SDD26 pKa = 5.51SPGGDD31 pKa = 2.74EE32 pKa = 5.72SGGNTNLQQNNNMTGVINPVTDD54 pKa = 3.44VDD56 pKa = 3.72WYY58 pKa = 10.6SIQVNEE64 pKa = 4.67PGLIGLNVFNEE75 pKa = 4.03TLRR78 pKa = 11.84YY79 pKa = 9.58DD80 pKa = 3.82VEE82 pKa = 4.27ILTTVYY88 pKa = 10.57EE89 pKa = 4.16KK90 pKa = 10.92DD91 pKa = 3.47ANGDD95 pKa = 3.9LQRR98 pKa = 11.84LAADD102 pKa = 4.35HH103 pKa = 6.48FPEE106 pKa = 5.37GSSANSSLTINVNVTQAKK124 pKa = 7.34TLYY127 pKa = 10.17IAVRR131 pKa = 11.84DD132 pKa = 4.04LDD134 pKa = 5.33DD135 pKa = 4.37NDD137 pKa = 4.17ASATEE142 pKa = 4.66TYY144 pKa = 10.42SISYY148 pKa = 10.3DD149 pKa = 3.34VAQAEE154 pKa = 4.53DD155 pKa = 3.75NNGSFDD161 pKa = 3.79TAVDD165 pKa = 3.94LALDD169 pKa = 4.94GACHH173 pKa = 6.52TDD175 pKa = 3.14SIGTVGDD182 pKa = 3.34VDD184 pKa = 3.74VGQFSLSNSGVYY196 pKa = 9.81EE197 pKa = 4.08ISADD201 pKa = 3.99FSAFAGGTAVDD212 pKa = 4.72LKK214 pKa = 11.33LSLFDD219 pKa = 4.88NEE221 pKa = 4.17GTLIRR226 pKa = 11.84SMGQTEE232 pKa = 4.0NSVYY236 pKa = 10.57RR237 pKa = 11.84LVEE240 pKa = 4.08SLPAGSFYY248 pKa = 11.48VLVHH252 pKa = 6.48DD253 pKa = 5.06QGKK256 pKa = 10.51DD257 pKa = 3.48DD258 pKa = 5.71FDD260 pKa = 4.0TASPFDD266 pKa = 3.88LCLSSVASAEE276 pKa = 4.0VSTDD280 pKa = 3.6DD281 pKa = 4.03NQQNATNLAGNGHH294 pKa = 5.99FEE296 pKa = 4.01ITGSIDD302 pKa = 3.31YY303 pKa = 11.03AGDD306 pKa = 3.69QDD308 pKa = 3.38WSSINSGATPPSLQVLQIEE327 pKa = 5.05FDD329 pKa = 3.64PSDD332 pKa = 4.55ANGCDD337 pKa = 2.89SWFLMEE343 pKa = 5.78VMDD346 pKa = 5.26SNDD349 pKa = 2.81VVLFSKK355 pKa = 10.44EE356 pKa = 3.9YY357 pKa = 8.97STEE360 pKa = 3.92TGPRR364 pKa = 11.84TAHH367 pKa = 6.05IRR369 pKa = 11.84VEE371 pKa = 4.19SSGEE375 pKa = 3.74HH376 pKa = 5.07FVRR379 pKa = 11.84VSGINEE385 pKa = 4.32DD386 pKa = 3.73VCSLDD391 pKa = 3.2GTVGMAYY398 pKa = 10.2SATVDD403 pKa = 3.7SVNVLDD409 pKa = 5.16DD410 pKa = 4.31AEE412 pKa = 4.82LGEE415 pKa = 4.64GNNTINTAIEE425 pKa = 4.03LDD427 pKa = 3.59EE428 pKa = 4.34TTNTEE433 pKa = 4.0TEE435 pKa = 4.01ALLSYY440 pKa = 10.6IGDD443 pKa = 3.72NDD445 pKa = 3.59WYY447 pKa = 10.84RR448 pKa = 11.84ITLPSDD454 pKa = 3.19NAQDD458 pKa = 4.0RR459 pKa = 11.84ILEE462 pKa = 4.15IFIEE466 pKa = 4.46TDD468 pKa = 2.88SEE470 pKa = 4.85TPLEE474 pKa = 4.29YY475 pKa = 10.8YY476 pKa = 9.22VTVFNADD483 pKa = 3.91EE484 pKa = 4.24IVDD487 pKa = 3.82TFTARR492 pKa = 11.84NNEE495 pKa = 3.96TSPVSFKK502 pKa = 10.94SSYY505 pKa = 10.26FIPSTAGNTNSDD517 pKa = 4.24YY518 pKa = 10.7FVKK521 pKa = 10.45IVDD524 pKa = 4.27LQSDD528 pKa = 3.71EE529 pKa = 5.39ADD531 pKa = 2.9IDD533 pKa = 3.69NNYY536 pKa = 9.45TIRR539 pKa = 11.84TNSYY543 pKa = 8.4TVQTTAPAANTGKK556 pKa = 8.46VTAAVFNSEE565 pKa = 4.19SAEE568 pKa = 4.04KK569 pKa = 10.75SLAASDD575 pKa = 3.88NTSLSLVVEE584 pKa = 4.76ASDD587 pKa = 3.41QRR589 pKa = 11.84SYY591 pKa = 11.6GVNTEE596 pKa = 3.98AFTIDD601 pKa = 3.72KK602 pKa = 8.82ATLANADD609 pKa = 3.44STITVTLPWQSGYY622 pKa = 11.25VDD624 pKa = 3.85FHH626 pKa = 7.85KK627 pKa = 11.13DD628 pKa = 3.19RR629 pKa = 11.84DD630 pKa = 3.78WFKK633 pKa = 11.67LDD635 pKa = 3.19IPTLYY640 pKa = 10.55GAAGGSAEE648 pKa = 3.82SWYY651 pKa = 11.07VEE653 pKa = 4.11VQIEE657 pKa = 4.79LYY659 pKa = 10.65SPAPGSLVEE668 pKa = 4.31YY669 pKa = 10.38AWGLYY674 pKa = 9.62RR675 pKa = 11.84DD676 pKa = 3.85EE677 pKa = 5.03SSNAVINEE685 pKa = 3.77WEE687 pKa = 4.42GEE689 pKa = 4.05DD690 pKa = 5.61GIIASNGDD698 pKa = 3.12VSALVSALDD707 pKa = 4.4LMTPAANAEE716 pKa = 4.18PMWISHH722 pKa = 5.69EE723 pKa = 4.33VANVPYY729 pKa = 9.47YY730 pKa = 10.32LTVTDD735 pKa = 5.93IINQSTNIADD745 pKa = 3.88NDD747 pKa = 3.59WGYY750 pKa = 11.22DD751 pKa = 3.08QAYY754 pKa = 9.95YY755 pKa = 10.2MRR757 pKa = 11.84AQLVFHH763 pKa = 7.16EE764 pKa = 4.93GLDD767 pKa = 3.78RR768 pKa = 11.84PADD771 pKa = 3.42

Molecular weight: 83.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y6BKT2|A0A1Y6BKT2_9ALTE Cu(I)/Ag(I) efflux system membrane protein CusA/SilA OS=Alteromonadaceae bacterium Bs31 OX=1304903 GN=SAMN02745866_00996 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.31QLAAA44 pKa = 4.14

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4297

0

4297

1444979

18

5930

336.3

37.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.896 ± 0.042

1.089 ± 0.014

5.535 ± 0.044

6.488 ± 0.04

4.106 ± 0.026

7.129 ± 0.048

2.136 ± 0.019

5.884 ± 0.027

5.112 ± 0.043

10.235 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.02

4.345 ± 0.036

4.021 ± 0.028

4.105 ± 0.027

4.768 ± 0.035

7.663 ± 0.069

5.029 ± 0.04

6.7 ± 0.033

1.354 ± 0.017

3.17 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski