Xanthomonas phage Carpasina

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Carpasinavirus; Xanthomonas virus Carpasina

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S1GSQ5|A0A2S1GSQ5_9CAUD DNA ligase OS=Xanthomonas phage Carpasina OX=2163636 PE=4 SV=1
MM1 pKa = 7.24YY2 pKa = 10.15KK3 pKa = 10.09VEE5 pKa = 4.81LNPVPNQTVSFNLDD19 pKa = 2.97GAFWQIHH26 pKa = 5.46IFQSISFMCADD37 pKa = 3.22ISLNGRR43 pKa = 11.84RR44 pKa = 11.84VISGVRR50 pKa = 11.84CFGGFGLMPYY60 pKa = 10.44SYY62 pKa = 8.0MTAPNFGNFVFDD74 pKa = 6.08FDD76 pKa = 5.77ADD78 pKa = 3.41WTGFGSTCSLYY89 pKa = 10.87FLDD92 pKa = 4.32AVEE95 pKa = 4.73FKK97 pKa = 10.68EE98 pKa = 4.38FQSLMLSGEE107 pKa = 4.08

Molecular weight:
12.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S1GSU6|A0A2S1GSU6_9CAUD Uncharacterized protein OS=Xanthomonas phage Carpasina OX=2163636 PE=4 SV=1
MM1 pKa = 7.57DD2 pKa = 3.4QVAASGLIVAAASPSVKK19 pKa = 10.01LYY21 pKa = 9.94QKK23 pKa = 9.06MRR25 pKa = 11.84RR26 pKa = 11.84GEE28 pKa = 3.89LRR30 pKa = 11.84TPLKK34 pKa = 10.18AQSNRR39 pKa = 11.84RR40 pKa = 11.84QFAGIKK46 pKa = 10.11AEE48 pKa = 4.21SPKK51 pKa = 10.05PQQAPALKK59 pKa = 9.03PGLVSGEE66 pKa = 3.93

Molecular weight:
7.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

19008

55

942

221.0

24.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.238 ± 0.394

0.921 ± 0.085

5.898 ± 0.212

6.497 ± 0.325

3.967 ± 0.197

7.534 ± 0.374

1.563 ± 0.133

6.034 ± 0.265

5.871 ± 0.309

7.423 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.135

4.461 ± 0.221

4.24 ± 0.179

3.967 ± 0.144

5.324 ± 0.201

7.071 ± 0.253

5.492 ± 0.254

6.887 ± 0.197

1.268 ± 0.106

2.646 ± 0.133

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski