Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae; Acidaminococcus; Acidaminococcus fermentans

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2RL43|D2RL43_ACIFV 3D domain protein OS=Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4) OX=591001 GN=Acfer_1436 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.51FKK4 pKa = 11.07VNQDD8 pKa = 3.08TCIGCGLCEE17 pKa = 4.34GTCPAVFHH25 pKa = 5.94MTDD28 pKa = 2.93AGVAEE33 pKa = 5.99AIPDD37 pKa = 3.72DD38 pKa = 4.14VPAGEE43 pKa = 4.36EE44 pKa = 4.04ANAQAALEE52 pKa = 4.33GCPVGAIEE60 pKa = 4.97KK61 pKa = 10.64VEE63 pKa = 4.01GG64 pKa = 3.58

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2RN38|D2RN38_ACIFV DUF1653 domain-containing protein OS=Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4) OX=591001 GN=Acfer_0055 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTNRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.01KK14 pKa = 7.65THH16 pKa = 5.5GFRR19 pKa = 11.84EE20 pKa = 4.01RR21 pKa = 11.84MKK23 pKa = 10.39TLGGKK28 pKa = 8.74QVLKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2016

0

2016

669530

30

5904

332.1

36.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.038 ± 0.068

1.313 ± 0.028

5.394 ± 0.049

6.522 ± 0.061

3.864 ± 0.054

8.165 ± 0.06

2.025 ± 0.025

5.818 ± 0.05

5.95 ± 0.045

10.151 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.038

3.528 ± 0.063

4.255 ± 0.047

3.697 ± 0.038

5.32 ± 0.058

5.044 ± 0.055

5.447 ± 0.083

7.309 ± 0.043

1.037 ± 0.023

3.255 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski