Escherichia phage ECML-117

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Wifcevirus; Escherichia virus ECML-117

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7A8J5|I7A8J5_9CAUD Uncharacterized protein OS=Escherichia phage ECML-117 OX=1204521 PE=4 SV=1
MM1 pKa = 6.9ITLAAIRR8 pKa = 11.84SARR11 pKa = 11.84VEE13 pKa = 3.86TSEE16 pKa = 3.71NSNVFGEE23 pKa = 4.48TLEE26 pKa = 4.13SVYY29 pKa = 10.76LSNVDD34 pKa = 3.34ATIEE38 pKa = 4.38SIYY41 pKa = 10.84EE42 pKa = 4.13PINFLDD48 pKa = 3.95LYY50 pKa = 10.42KK51 pKa = 10.92YY52 pKa = 10.09RR53 pKa = 11.84DD54 pKa = 3.77EE55 pKa = 6.5DD56 pKa = 3.54IVSYY60 pKa = 10.57RR61 pKa = 11.84VTINGFVYY69 pKa = 10.0TITEE73 pKa = 3.66IGGEE77 pKa = 4.28YY78 pKa = 9.71IAVPQLGGEE87 pKa = 4.03VTYY90 pKa = 9.65FKK92 pKa = 10.53IVSLL96 pKa = 4.08

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6ZXV4|I6ZXV4_9CAUD Uncharacterized protein OS=Escherichia phage ECML-117 OX=1204521 PE=4 SV=1
MM1 pKa = 7.1NAALGKK7 pKa = 10.64LSFNKK12 pKa = 9.95RR13 pKa = 11.84NEE15 pKa = 3.61LRR17 pKa = 11.84MVSRR21 pKa = 11.84FQFLLNAMSSVAIAMLRR38 pKa = 11.84ADD40 pKa = 4.15CPAEE44 pKa = 3.96KK45 pKa = 10.29RR46 pKa = 11.84EE47 pKa = 4.16TLFKK51 pKa = 10.55RR52 pKa = 11.84HH53 pKa = 4.56QQLRR57 pKa = 11.84NAAYY61 pKa = 9.78KK62 pKa = 8.91LQQYY66 pKa = 10.17AMEE69 pKa = 4.17YY70 pKa = 10.9AKK72 pKa = 10.95

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

20156

45

965

214.4

23.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.717 ± 0.325

0.933 ± 0.087

5.983 ± 0.192

6.589 ± 0.377

4.004 ± 0.142

7.521 ± 0.29

1.573 ± 0.144

6.415 ± 0.188

6.445 ± 0.369

7.368 ± 0.183

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.148

5.065 ± 0.214

3.746 ± 0.131

3.84 ± 0.172

4.922 ± 0.242

6.336 ± 0.287

5.854 ± 0.299

7.055 ± 0.211

1.295 ± 0.096

3.503 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski