Mycobacterium phage Turbido

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; Mycobacterium virus Turbido

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1JUX6|G1JUX6_9CAUD Uncharacterized protein OS=Mycobacterium phage Turbido OX=1071504 GN=10 PE=4 SV=1
MM1 pKa = 7.66LGQDD5 pKa = 3.55SNYY8 pKa = 8.32QQKK11 pKa = 10.94SYY13 pKa = 11.01VFEE16 pKa = 4.38AAASVTTVAGRR27 pKa = 11.84EE28 pKa = 3.92QASADD33 pKa = 2.97IDD35 pKa = 4.08YY36 pKa = 10.87LVSLITNPGGSAYY49 pKa = 9.85MVMIGVDD56 pKa = 4.08LEE58 pKa = 4.47VPLPSGQVAVDD69 pKa = 3.63TKK71 pKa = 10.89ISTLSLPADD80 pKa = 4.45DD81 pKa = 4.52YY82 pKa = 11.75TPLFDD87 pKa = 4.9FVGPLEE93 pKa = 4.22EE94 pKa = 4.19AA95 pKa = 3.8

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1JUS9|G1JUS9_9CAUD Metallophosphoesterase OS=Mycobacterium phage Turbido OX=1071504 GN=57 PE=4 SV=1
MM1 pKa = 7.55TSTLPYY7 pKa = 8.53LHH9 pKa = 6.93KK10 pKa = 10.31NARR13 pKa = 11.84SRR15 pKa = 11.84RR16 pKa = 11.84ITRR19 pKa = 11.84KK20 pKa = 7.77EE21 pKa = 3.62VRR23 pKa = 11.84EE24 pKa = 4.0VFAAEE29 pKa = 3.86VTRR32 pKa = 11.84NLDD35 pKa = 3.09RR36 pKa = 11.84RR37 pKa = 11.84LDD39 pKa = 3.6RR40 pKa = 11.84EE41 pKa = 3.87EE42 pKa = 3.98YY43 pKa = 9.8LRR45 pKa = 11.84KK46 pKa = 10.25VMPP49 pKa = 4.57

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16400

37

847

174.5

19.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.976 ± 0.315

0.933 ± 0.119

6.494 ± 0.209

6.841 ± 0.356

3.451 ± 0.164

7.75 ± 0.291

1.982 ± 0.194

4.988 ± 0.165

4.75 ± 0.222

8.433 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.125

3.323 ± 0.198

5.445 ± 0.222

3.494 ± 0.213

6.463 ± 0.411

5.238 ± 0.221

6.152 ± 0.23

6.988 ± 0.223

1.866 ± 0.138

2.89 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski