Merkel cell polyomavirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus; Human polyomavirus 5

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6DVW7|B6DVW7_9POLY Large T antigen OS=Merkel cell polyomavirus OX=493803 GN=LT PE=4 SV=1
MM1 pKa = 7.42GGIITLLANIGEE13 pKa = 4.47IATEE17 pKa = 4.46LSATTGVTLEE27 pKa = 4.9AILTGEE33 pKa = 4.08ALAALEE39 pKa = 4.46AEE41 pKa = 4.71ISSLMTIEE49 pKa = 4.98GISGIEE55 pKa = 3.86ALAQLGFTAEE65 pKa = 4.04QFSNFSLVASLVNQGLTYY83 pKa = 10.85GFILQTVSGIGSLITVGVRR102 pKa = 11.84LSRR105 pKa = 11.84EE106 pKa = 3.78QVSLVKK112 pKa = 10.22RR113 pKa = 11.84DD114 pKa = 3.77VSWVGSNEE122 pKa = 4.12VLRR125 pKa = 11.84HH126 pKa = 5.17ALMAFSLDD134 pKa = 3.38PLQWEE139 pKa = 4.2NSLLHH144 pKa = 6.91SVGQDD149 pKa = 2.56IFNSLSPTSRR159 pKa = 11.84LQIQSNLVNLILNSRR174 pKa = 11.84WVFQTTASQNQGLLSGEE191 pKa = 4.68AILIPEE197 pKa = 5.06HH198 pKa = 7.11IGGTLQQQTPDD209 pKa = 2.97WLLPLVLGLSGYY221 pKa = 10.08ISPEE225 pKa = 3.71LQVIEE230 pKa = 5.5DD231 pKa = 3.79GTKK234 pKa = 9.66KK235 pKa = 10.51KK236 pKa = 10.91SIIHH240 pKa = 6.3LL241 pKa = 3.99

Molecular weight: 25.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0G0W3|B0G0W3_9POLY Capsid protein VP1 OS=Merkel cell polyomavirus OX=493803 GN=VP1 PE=1 SV=1
MM1 pKa = 7.97DD2 pKa = 5.16LVLNRR7 pKa = 11.84KK8 pKa = 7.67EE9 pKa = 4.64RR10 pKa = 11.84EE11 pKa = 4.15ALCKK15 pKa = 10.38LLEE18 pKa = 4.35IAPNCYY24 pKa = 10.74GNIPLMKK31 pKa = 10.1AAFKK35 pKa = 10.5RR36 pKa = 11.84SCLKK40 pKa = 10.33HH41 pKa = 7.01HH42 pKa = 7.26PDD44 pKa = 3.13KK45 pKa = 11.37GGNPVIMMEE54 pKa = 5.71LNTLWSKK61 pKa = 9.27FQQNIHH67 pKa = 6.44KK68 pKa = 9.99LRR70 pKa = 11.84SDD72 pKa = 3.41FSMFDD77 pKa = 3.34EE78 pKa = 5.09VSTKK82 pKa = 10.06FPWEE86 pKa = 3.89EE87 pKa = 3.79YY88 pKa = 9.07GTLKK92 pKa = 10.89DD93 pKa = 4.0YY94 pKa = 8.55MQSGYY99 pKa = 8.71NARR102 pKa = 11.84FCRR105 pKa = 11.84GPGCMLKK112 pKa = 9.77QLRR115 pKa = 11.84DD116 pKa = 3.82SKK118 pKa = 10.64CACISCKK125 pKa = 10.1LSRR128 pKa = 11.84QHH130 pKa = 7.32CSLKK134 pKa = 9.74TLKK137 pKa = 10.34QKK139 pKa = 10.77NCLTWGEE146 pKa = 4.47CFCYY150 pKa = 10.15QCFILWFGFPPTWEE164 pKa = 4.29SFDD167 pKa = 3.03WWQKK171 pKa = 7.04TLEE174 pKa = 4.15EE175 pKa = 3.65TDD177 pKa = 3.4YY178 pKa = 11.58CLLHH182 pKa = 6.32LHH184 pKa = 6.88LFF186 pKa = 4.12

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1667

186

817

416.8

46.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.919 ± 0.43

2.879 ± 0.845

4.379 ± 0.507

6.779 ± 0.795

4.319 ± 0.803

6.119 ± 1.138

2.1 ± 0.417

5.219 ± 0.817

7.259 ± 1.224

10.198 ± 1.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.04 ± 0.274

4.859 ± 0.193

6.419 ± 1.012

4.559 ± 0.764

3.719 ± 0.491

9.658 ± 1.103

5.699 ± 0.497

4.499 ± 1.488

1.5 ± 0.325

2.879 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski