Butyricicoccus porcorum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Butyricicoccus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A252F4M6|A0A252F4M6_9CLOT PspC domain-containing protein OS=Butyricicoccus porcorum OX=1945634 GN=CBW42_07830 PE=4 SV=1
MM1 pKa = 7.31TKK3 pKa = 8.4NTKK6 pKa = 9.9RR7 pKa = 11.84LLFGVIAVAVLGGTYY22 pKa = 10.36AALLLHH28 pKa = 6.49PAEE31 pKa = 4.76EE32 pKa = 4.6EE33 pKa = 4.0ADD35 pKa = 3.83SVPSEE40 pKa = 4.36EE41 pKa = 4.52LVSMDD46 pKa = 3.56TADD49 pKa = 3.74LASVQVTLRR58 pKa = 11.84DD59 pKa = 3.64GSSFTMTTSSDD70 pKa = 3.71DD71 pKa = 3.45SGTSYY76 pKa = 10.91TMSGASEE83 pKa = 4.33DD84 pKa = 4.55DD85 pKa = 3.7YY86 pKa = 11.87SEE88 pKa = 5.27SLMQGLMDD96 pKa = 4.89AACDD100 pKa = 3.31ISARR104 pKa = 11.84PIEE107 pKa = 4.5EE108 pKa = 4.17TCSDD112 pKa = 3.77PEE114 pKa = 4.23KK115 pKa = 10.67YY116 pKa = 10.45GLSAEE121 pKa = 4.94DD122 pKa = 3.61DD123 pKa = 4.05TNTLVITDD131 pKa = 3.78TDD133 pKa = 3.83GTAVTLTIGLSGEE146 pKa = 4.08LGTYY150 pKa = 10.45CSTNGGTAVYY160 pKa = 10.62LLDD163 pKa = 4.31SDD165 pKa = 4.12TAEE168 pKa = 4.58TLTQAQSYY176 pKa = 8.34YY177 pKa = 11.05RR178 pKa = 11.84NLTVLGGYY186 pKa = 10.04YY187 pKa = 10.4SLSDD191 pKa = 3.66EE192 pKa = 4.79LKK194 pKa = 10.83SLTIDD199 pKa = 3.62HH200 pKa = 6.93MDD202 pKa = 4.54DD203 pKa = 3.42GTTVTLSARR212 pKa = 11.84DD213 pKa = 3.82TSDD216 pKa = 2.87MDD218 pKa = 4.12SDD220 pKa = 4.0VAQAYY225 pKa = 9.6SKK227 pKa = 11.26YY228 pKa = 10.9VFTAPLSCDD237 pKa = 3.42ADD239 pKa = 4.12DD240 pKa = 6.02DD241 pKa = 4.27EE242 pKa = 6.63LSTGLLSGLQSGLTAQSIVEE262 pKa = 4.74DD263 pKa = 4.01NPSDD267 pKa = 3.66LSRR270 pKa = 11.84YY271 pKa = 9.93GLDD274 pKa = 3.33DD275 pKa = 3.38PTRR278 pKa = 11.84IEE280 pKa = 4.18LAASSLDD287 pKa = 3.49AAILIGDD294 pKa = 3.9EE295 pKa = 4.21TDD297 pKa = 3.61DD298 pKa = 3.87GGIYY302 pKa = 10.29VMKK305 pKa = 10.44EE306 pKa = 3.61GGKK309 pKa = 7.65TVYY312 pKa = 10.65LCTASDD318 pKa = 3.84YY319 pKa = 11.78SFLEE323 pKa = 4.07EE324 pKa = 5.48DD325 pKa = 3.31WNDD328 pKa = 3.12WRR330 pKa = 11.84STNLLPCALSEE341 pKa = 4.08VDD343 pKa = 5.63KK344 pKa = 10.83ITVTQGDD351 pKa = 3.81EE352 pKa = 4.06THH354 pKa = 6.48TVDD357 pKa = 3.33ITHH360 pKa = 6.28VEE362 pKa = 3.81ADD364 pKa = 3.76EE365 pKa = 4.82NEE367 pKa = 4.24SDD369 pKa = 5.52DD370 pKa = 5.74ADD372 pKa = 5.21DD373 pKa = 5.16DD374 pKa = 4.25TATLDD379 pKa = 3.92GADD382 pKa = 3.62MTDD385 pKa = 3.57DD386 pKa = 3.81ALEE389 pKa = 4.06QFFLAITSVNYY400 pKa = 9.09TRR402 pKa = 11.84LIDD405 pKa = 4.48DD406 pKa = 4.71PQDD409 pKa = 3.2AAASATVTLTMNDD422 pKa = 2.83GSTRR426 pKa = 11.84SLAFTKK432 pKa = 10.52GGSRR436 pKa = 11.84EE437 pKa = 3.97YY438 pKa = 10.73LVSVDD443 pKa = 3.34GGAYY447 pKa = 10.06AYY449 pKa = 8.84GVPQDD454 pKa = 4.81DD455 pKa = 4.13LTSILDD461 pKa = 3.83ALTTGAA467 pKa = 4.93

Molecular weight: 49.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A252F519|A0A252F519_9CLOT Bifunctional protein FolD OS=Butyricicoccus porcorum OX=1945634 GN=folD PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.55KK9 pKa = 7.8RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.92GFRR19 pKa = 11.84KK20 pKa = 10.08RR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.83VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.15RR41 pKa = 11.84LTHH44 pKa = 6.25

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2566

0

2566

777331

37

1759

302.9

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.252 ± 0.055

1.864 ± 0.025

5.913 ± 0.038

6.677 ± 0.053

3.794 ± 0.036

7.453 ± 0.046

2.033 ± 0.023

6.353 ± 0.044

5.051 ± 0.049

9.114 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.023 ± 0.022

3.526 ± 0.032

3.802 ± 0.036

3.611 ± 0.028

5.468 ± 0.061

5.749 ± 0.041

5.794 ± 0.046

6.988 ± 0.042

0.924 ± 0.016

3.608 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski