Coleus vein necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7TZR9|A7TZR9_9VIRU Movement protein TGB2 OS=Coleus vein necrosis virus OX=404404 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 4.4FVCTVLQEE10 pKa = 4.48FGFSSVSVKK19 pKa = 10.38FGRR22 pKa = 11.84PIVVNCVPGSGKK34 pKa = 7.86SHH36 pKa = 7.92CIRR39 pKa = 11.84TILGRR44 pKa = 11.84DD45 pKa = 3.27SRR47 pKa = 11.84FVAATFGKK55 pKa = 10.16ADD57 pKa = 5.14PINVHH62 pKa = 6.05CRR64 pKa = 11.84RR65 pKa = 11.84IASTSEE71 pKa = 3.58IEE73 pKa = 4.41GLDD76 pKa = 3.33SKK78 pKa = 11.16YY79 pKa = 10.69IIIDD83 pKa = 4.09EE84 pKa = 4.32YY85 pKa = 11.7QLGSWEE91 pKa = 4.33AFDD94 pKa = 5.43PIAIFGDD101 pKa = 4.05PCQGTAPCIPPQFISTKK118 pKa = 6.84TRR120 pKa = 11.84RR121 pKa = 11.84FGYY124 pKa = 7.49NTCGLLQKK132 pKa = 10.49FGFSIEE138 pKa = 4.08SDD140 pKa = 3.44QEE142 pKa = 4.33DD143 pKa = 3.84EE144 pKa = 4.93VVIQSSAVGEE154 pKa = 4.35VEE156 pKa = 5.03GEE158 pKa = 4.22VLACGPEE165 pKa = 3.95AEE167 pKa = 6.15LILTWYY173 pKa = 9.66GYY175 pKa = 9.34PYY177 pKa = 10.72KK178 pKa = 10.37KK179 pKa = 9.84YY180 pKa = 10.5CEE182 pKa = 4.19VRR184 pKa = 11.84GSTFEE189 pKa = 4.23VVTLVTDD196 pKa = 4.3FSTIPEE202 pKa = 4.15EE203 pKa = 4.62LRR205 pKa = 11.84VEE207 pKa = 5.12LYY209 pKa = 9.73TCLTRR214 pKa = 11.84HH215 pKa = 5.48RR216 pKa = 11.84RR217 pKa = 11.84KK218 pKa = 10.46LIIINGDD225 pKa = 3.08ATFAPAGQHH234 pKa = 5.58

Molecular weight: 25.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7TZS2|A7TZS2_9VIRU Nucleic acid binding protein OS=Coleus vein necrosis virus OX=404404 PE=4 SV=1
MM1 pKa = 6.97VQTTQIDD8 pKa = 3.88MEE10 pKa = 4.54DD11 pKa = 3.98LEE13 pKa = 4.9FTLCVCKK20 pKa = 10.57IKK22 pKa = 10.75LSEE25 pKa = 4.57LGLPTSVCHH34 pKa = 6.64YY35 pKa = 10.56ILDD38 pKa = 4.12PLVAAVRR45 pKa = 11.84TCLRR49 pKa = 11.84QEE51 pKa = 4.29KK52 pKa = 9.69QRR54 pKa = 11.84QNAPFNGVSRR64 pKa = 11.84SSAKK68 pKa = 9.96RR69 pKa = 11.84RR70 pKa = 11.84AKK72 pKa = 10.86YY73 pKa = 10.26LDD75 pKa = 3.28RR76 pKa = 11.84CIKK79 pKa = 10.18CGKK82 pKa = 9.54FSHH85 pKa = 6.91TGPCPRR91 pKa = 11.84NQTISNAEE99 pKa = 4.02IVHH102 pKa = 6.78LIYY105 pKa = 11.05NGTTRR110 pKa = 11.84FLAEE114 pKa = 3.78KK115 pKa = 8.35PQYY118 pKa = 10.23RR119 pKa = 11.84RR120 pKa = 11.84GSFAQMLADD129 pKa = 4.06RR130 pKa = 11.84LLEE133 pKa = 4.1RR134 pKa = 11.84SNLSLL139 pKa = 4.34

Molecular weight: 15.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2841

68

1977

473.5

52.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.673 ± 1.352

2.746 ± 0.609

4.611 ± 0.336

6.582 ± 0.706

5.702 ± 0.674

6.969 ± 0.394

2.534 ± 0.466

5.139 ± 0.76

6.019 ± 0.572

9.996 ± 1.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.343

3.942 ± 0.547

4.329 ± 0.462

2.992 ± 0.335

5.385 ± 0.582

7.392 ± 0.146

5.702 ± 1.02

6.512 ± 0.509

0.774 ± 0.081

2.957 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski