Mycobacterium virus Gumbie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1JTM4|G1JTM4_9CAUD Uncharacterized protein OS=Mycobacterium virus Gumbie OX=1071505 GN=78 PE=4 SV=1
MM1 pKa = 7.92SGEE4 pKa = 4.04INPEE8 pKa = 3.43GFTRR12 pKa = 11.84YY13 pKa = 10.28GGDD16 pKa = 3.72CTCGPIYY23 pKa = 10.21TYY25 pKa = 10.79GGHH28 pKa = 7.13AEE30 pKa = 4.48PGQFDD35 pKa = 4.88PFCPDD40 pKa = 2.8HH41 pKa = 6.95GNPEE45 pKa = 4.29YY46 pKa = 10.83VASLEE51 pKa = 4.27EE52 pKa = 4.01AA53 pKa = 3.67

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1JTL5|G1JTL5_9CAUD Uncharacterized protein OS=Mycobacterium virus Gumbie OX=1071505 GN=69 PE=4 SV=1
MM1 pKa = 7.44PHH3 pKa = 6.75GNGLRR8 pKa = 11.84VGNRR12 pKa = 11.84ACMFSVAGQQPVANQKK28 pKa = 6.55THH30 pKa = 5.63SLEE33 pKa = 4.03TAMTRR38 pKa = 11.84GFAIKK43 pKa = 9.16MGRR46 pKa = 11.84VKK48 pKa = 10.71RR49 pKa = 11.84EE50 pKa = 3.61MKK52 pKa = 10.36KK53 pKa = 10.46RR54 pKa = 11.84GIPDD58 pKa = 3.23YY59 pKa = 11.02AALARR64 pKa = 11.84DD65 pKa = 3.55MKK67 pKa = 10.9VNKK70 pKa = 8.55STVWRR75 pKa = 11.84VMNGRR80 pKa = 11.84ARR82 pKa = 11.84PGPDD86 pKa = 3.24FVNALLDD93 pKa = 3.49AWDD96 pKa = 4.83LEE98 pKa = 4.34FHH100 pKa = 7.42DD101 pKa = 5.95LFDD104 pKa = 5.18DD105 pKa = 4.19PRR107 pKa = 11.84KK108 pKa = 9.91LRR110 pKa = 11.84PKK112 pKa = 10.13RR113 pKa = 11.84AAA115 pKa = 3.41

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

18437

31

1161

177.3

19.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.36 ± 0.483

1.345 ± 0.162

6.536 ± 0.225

5.966 ± 0.304

2.951 ± 0.226

8.732 ± 0.566

2.462 ± 0.21

4.366 ± 0.172

3.433 ± 0.201

7.165 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.114

3.379 ± 0.181

6.026 ± 0.207

3.482 ± 0.177

6.856 ± 0.342

5.76 ± 0.274

6.666 ± 0.214

7.214 ± 0.291

2.332 ± 0.153

2.658 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski