Enterococcus phage phiFL2A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Phifelvirus; Enterococcus virus FL2

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2IZD6|D2IZD6_9CAUD Endonuclease YqaJ superfamily OS=Enterococcus phage phiFL2A OX=673835 GN=gp16 PE=4 SV=1
MM1 pKa = 7.97PEE3 pKa = 4.04FDD5 pKa = 4.18SLGARR10 pKa = 11.84QEE12 pKa = 4.01PPEE15 pKa = 4.09EE16 pKa = 4.46KK17 pKa = 10.14EE18 pKa = 4.05VLEE21 pKa = 4.3LTWEE25 pKa = 4.03YY26 pKa = 11.47DD27 pKa = 3.44EE28 pKa = 6.21EE29 pKa = 4.37EE30 pKa = 4.49DD31 pKa = 4.94EE32 pKa = 4.53RR33 pKa = 5.78

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2IZE2|D2IZE2_9CAUD Uncharacterized protein gp22 OS=Enterococcus phage phiFL2A OX=673835 GN=gp22 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84ISLPIEE8 pKa = 4.32PKK10 pKa = 8.52PQSRR14 pKa = 11.84PRR16 pKa = 11.84FARR19 pKa = 11.84RR20 pKa = 11.84GNYY23 pKa = 7.94VQTYY27 pKa = 8.75EE28 pKa = 4.16DD29 pKa = 3.35SAMRR33 pKa = 11.84AYY35 pKa = 9.36KK36 pKa = 10.24QKK38 pKa = 10.41IKK40 pKa = 10.81AYY42 pKa = 9.39LRR44 pKa = 11.84KK45 pKa = 9.87AKK47 pKa = 10.27PEE49 pKa = 4.59LIEE52 pKa = 4.39KK53 pKa = 10.11GAIFAHH59 pKa = 4.87VTFYY63 pKa = 11.47VPAPKK68 pKa = 10.44YY69 pKa = 10.54LLSSKK74 pKa = 10.36KK75 pKa = 9.93KK76 pKa = 8.38RR77 pKa = 11.84LEE79 pKa = 3.95VKK81 pKa = 10.61LEE83 pKa = 3.79RR84 pKa = 11.84KK85 pKa = 9.89YY86 pKa = 11.02CDD88 pKa = 3.55KK89 pKa = 11.18KK90 pKa = 11.39SDD92 pKa = 3.43LDD94 pKa = 3.85NYY96 pKa = 9.98FKK98 pKa = 11.05AVTDD102 pKa = 3.63AAEE105 pKa = 5.05GILYY109 pKa = 10.68KK110 pKa = 10.46NDD112 pKa = 3.08GQIAVMVCQKK122 pKa = 10.65LYY124 pKa = 10.85SLNPRR129 pKa = 11.84TEE131 pKa = 3.74IEE133 pKa = 3.83IMRR136 pKa = 11.84LEE138 pKa = 4.52GEE140 pKa = 4.13

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

10659

30

706

169.2

19.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.586 ± 0.399

0.572 ± 0.094

5.864 ± 0.325

7.674 ± 0.579

3.978 ± 0.238

6.239 ± 0.373

1.21 ± 0.144

7.008 ± 0.311

8.659 ± 0.49

8.481 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.239

6.52 ± 0.585

2.721 ± 0.196

4.259 ± 0.281

3.818 ± 0.382

6.333 ± 0.515

6.38 ± 0.42

6.145 ± 0.204

1.051 ± 0.13

3.912 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski