Yersinia phage phi80-18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Pokrovskaiavirus; Yersinia virus Phi80-18

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7LHC2|I7LHC2_9CAUD Uncharacterized protein OS=Yersinia phage phi80-18 OX=1206559 GN=g08 PE=4 SV=2
MM1 pKa = 7.66IKK3 pKa = 8.46HH4 pKa = 5.88TLATSFTLKK13 pKa = 10.5VMNLDD18 pKa = 3.46TDD20 pKa = 3.87DD21 pKa = 5.47SYY23 pKa = 12.02TLYY26 pKa = 10.88FDD28 pKa = 3.78TQAEE32 pKa = 3.97LDD34 pKa = 3.73AAVQAVRR41 pKa = 11.84EE42 pKa = 4.11LASIINTTLRR52 pKa = 11.84ISISSTQDD60 pKa = 2.67YY61 pKa = 11.33DD62 pKa = 3.59LL63 pKa = 5.76

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7J422|I7J422_9CAUD Uncharacterized protein OS=Yersinia phage phi80-18 OX=1206559 GN=g22 PE=4 SV=2
MM1 pKa = 7.26VNLHH5 pKa = 6.3IIFRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84LTDD14 pKa = 3.59FLFNPKK20 pKa = 9.85RR21 pKa = 11.84PNEE24 pKa = 3.95VTFKK28 pKa = 10.83NVYY31 pKa = 6.95ITHH34 pKa = 6.18RR35 pKa = 11.84TGGLEE40 pKa = 4.04VKK42 pKa = 10.67HH43 pKa = 6.62NDD45 pKa = 2.86GKK47 pKa = 10.85VYY49 pKa = 10.02IYY51 pKa = 10.65SWHH54 pKa = 5.55TLNRR58 pKa = 11.84VRR60 pKa = 11.84TEE62 pKa = 3.65HH63 pKa = 6.63CC64 pKa = 3.81

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13480

42

1259

236.5

26.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.555 ± 0.551

1.157 ± 0.173

6.372 ± 0.19

6.269 ± 0.264

3.516 ± 0.17

7.218 ± 0.319

2.033 ± 0.186

4.896 ± 0.256

6.298 ± 0.283

8.301 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.138 ± 0.179

4.303 ± 0.227

3.746 ± 0.17

4.206 ± 0.26

5.371 ± 0.213

6.068 ± 0.236

5.734 ± 0.249

6.914 ± 0.395

1.35 ± 0.115

3.546 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski