Clostridium sp. CAG:138

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6AWP7|R6AWP7_9CLOT Serine-type D-Ala-D-Ala carboxypeptidase OS=Clostridium sp. CAG:138 OX=1262775 GN=BN491_01416 PE=4 SV=1
MM1 pKa = 7.56LVPVVLSADD10 pKa = 3.61GEE12 pKa = 4.89YY13 pKa = 10.99VLSPLDD19 pKa = 3.59SSEE22 pKa = 5.47SMDD25 pKa = 3.92NLLWTANGDD34 pKa = 3.71YY35 pKa = 11.2TNGFSFRR42 pKa = 11.84SVGSGLYY49 pKa = 9.83FAGLTYY55 pKa = 10.77CNWTAINQTEE65 pKa = 4.3DD66 pKa = 2.64LWLPLEE72 pKa = 4.55HH73 pKa = 7.11TDD75 pKa = 3.94GGTAKK80 pKa = 9.97TILKK84 pKa = 9.77SVRR87 pKa = 11.84AEE89 pKa = 4.17QIDD92 pKa = 3.76PTAVKK97 pKa = 9.79LYY99 pKa = 10.9AGITTGYY106 pKa = 8.58FNSVIFDD113 pKa = 3.47YY114 pKa = 11.41VRR116 pKa = 11.84IADD119 pKa = 3.74AANVEE124 pKa = 4.89FYY126 pKa = 11.08KK127 pKa = 10.77FGSSGSEE134 pKa = 3.87YY135 pKa = 11.27ALGDD139 pKa = 3.54VNMDD143 pKa = 3.46GSVSVSDD150 pKa = 3.68ALMVLRR156 pKa = 11.84FTMGLEE162 pKa = 4.19TLSDD166 pKa = 3.83AQLEE170 pKa = 4.25LADD173 pKa = 4.28FNGDD177 pKa = 3.67GTVSTVDD184 pKa = 3.15ALDD187 pKa = 3.44ILRR190 pKa = 11.84AAII193 pKa = 3.89

Molecular weight: 20.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6AS43|R6AS43_9CLOT Sel1 repeat OS=Clostridium sp. CAG:138 OX=1262775 GN=BN491_00695 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.69MTFQPKK8 pKa = 8.3VRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.44KK14 pKa = 7.89VHH16 pKa = 5.68GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSTANGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.76GRR39 pKa = 11.84KK40 pKa = 8.74KK41 pKa = 10.66LSAA44 pKa = 3.95

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1746

0

1746

548493

29

2177

314.1

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.492 ± 0.06

1.914 ± 0.031

5.637 ± 0.046

6.967 ± 0.057

4.281 ± 0.042

7.434 ± 0.053

1.695 ± 0.022

6.712 ± 0.047

5.472 ± 0.05

9.006 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.708 ± 0.026

4.349 ± 0.034

3.727 ± 0.035

2.517 ± 0.027

5.469 ± 0.048

6.253 ± 0.049

5.342 ± 0.048

6.841 ± 0.045

0.711 ± 0.019

3.469 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski