Clostridium pasteurianum DSM 525 = ATCC 6013

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium pasteurianum

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3JAK1|A0A0H3JAK1_CLOPA Putative transcriptional regulator OS=Clostridium pasteurianum DSM 525 = ATCC 6013 OX=1262449 GN=CLPA_c25660 PE=4 SV=1
MM1 pKa = 7.26TLDD4 pKa = 3.28EE5 pKa = 4.59VKK7 pKa = 10.87NYY9 pKa = 10.47LRR11 pKa = 11.84VDD13 pKa = 3.1ADD15 pKa = 4.32FIEE18 pKa = 5.12DD19 pKa = 3.54DD20 pKa = 4.85AYY22 pKa = 10.15IQTLIDD28 pKa = 3.44NSQTYY33 pKa = 10.12IDD35 pKa = 4.12SCVGTGYY42 pKa = 8.25QTNANLVKK50 pKa = 10.57LATTAQYY57 pKa = 11.84KK58 pKa = 10.3LINDD62 pKa = 4.7LYY64 pKa = 10.53NNRR67 pKa = 11.84SQYY70 pKa = 10.57IDD72 pKa = 3.31GSFKK76 pKa = 10.58RR77 pKa = 11.84DD78 pKa = 3.45IIIDD82 pKa = 4.01TIFDD86 pKa = 3.82MLASASDD93 pKa = 3.73EE94 pKa = 4.51VII96 pKa = 3.65

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3IXS3|A0A0H3IXS3_CLOPA G5 domain-containing protein OS=Clostridium pasteurianum DSM 525 = ATCC 6013 OX=1262449 GN=CLPA_c01710 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84EE4 pKa = 4.11NGNSRR9 pKa = 11.84DD10 pKa = 3.63GRR12 pKa = 11.84DD13 pKa = 3.32SRR15 pKa = 11.84KK16 pKa = 9.93GGPRR20 pKa = 11.84GRR22 pKa = 11.84RR23 pKa = 11.84MKK25 pKa = 10.65RR26 pKa = 11.84KK27 pKa = 9.0VCSFCMDD34 pKa = 3.42KK35 pKa = 10.98ADD37 pKa = 4.68HH38 pKa = 7.03IDD40 pKa = 3.49YY41 pKa = 11.12KK42 pKa = 11.11DD43 pKa = 3.21INKK46 pKa = 9.01LRR48 pKa = 11.84KK49 pKa = 9.78YY50 pKa = 8.01ITEE53 pKa = 4.01RR54 pKa = 11.84GKK56 pKa = 10.04ILPRR60 pKa = 11.84RR61 pKa = 11.84ISGNCAKK68 pKa = 9.64HH69 pKa = 5.56QRR71 pKa = 11.84MLTEE75 pKa = 4.16SIKK78 pKa = 10.25RR79 pKa = 11.84SRR81 pKa = 11.84NIALLPFTTEE91 pKa = 3.59

Molecular weight: 10.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3930

0

3930

1177515

29

3107

299.6

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.62 ± 0.043

1.189 ± 0.016

5.498 ± 0.031

6.958 ± 0.048

4.457 ± 0.028

6.246 ± 0.042

1.411 ± 0.015

10.315 ± 0.048

9.19 ± 0.037

8.783 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.02

6.599 ± 0.045

2.827 ± 0.024

2.395 ± 0.022

3.438 ± 0.025

6.569 ± 0.036

4.828 ± 0.034

6.225 ± 0.032

0.726 ± 0.014

4.187 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski