Duncaniella muris

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; Duncaniella

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V1INT2|A0A2V1INT2_9BACT AhpC/TSA family protein OS=Duncaniella muris OX=2094150 GN=C5O23_05780 PE=4 SV=1
MM1 pKa = 7.52SKK3 pKa = 9.27TYY5 pKa = 10.58YY6 pKa = 10.65SPTHH10 pKa = 6.31EE11 pKa = 4.95YY12 pKa = 8.66ITVDD16 pKa = 3.45GNIGYY21 pKa = 9.73IGISDD26 pKa = 4.16YY27 pKa = 11.27AQHH30 pKa = 6.95ALGNVVYY37 pKa = 11.05VDD39 pKa = 4.07MPDD42 pKa = 3.31EE43 pKa = 4.52GDD45 pKa = 3.61EE46 pKa = 4.21IEE48 pKa = 4.58AGEE51 pKa = 4.29DD52 pKa = 3.34FGAVEE57 pKa = 4.41SVKK60 pKa = 10.67AASDD64 pKa = 4.32LIAPVSGEE72 pKa = 3.82VLEE75 pKa = 5.15ANEE78 pKa = 4.58KK79 pKa = 10.87LSDD82 pKa = 3.65NPRR85 pKa = 11.84LINEE89 pKa = 4.3DD90 pKa = 4.46AMANWIIKK98 pKa = 10.12VEE100 pKa = 4.05LTDD103 pKa = 5.42LSQLDD108 pKa = 3.65SLMDD112 pKa = 3.48EE113 pKa = 4.62EE114 pKa = 5.6AYY116 pKa = 9.71KK117 pKa = 10.62AHH119 pKa = 6.99CEE121 pKa = 3.95AEE123 pKa = 4.29KK124 pKa = 11.01

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V1IJ92|A0A2V1IJ92_9BACT Uncharacterized protein OS=Duncaniella muris OX=2094150 GN=C5O23_09310 PE=4 SV=1
MM1 pKa = 7.51TGFLISIKK9 pKa = 10.33HH10 pKa = 5.95RR11 pKa = 11.84FPAIWRR17 pKa = 11.84AVEE20 pKa = 3.88WANGKK25 pKa = 10.03AMRR28 pKa = 11.84LRR30 pKa = 11.84YY31 pKa = 8.99PRR33 pKa = 11.84LGMIATEE40 pKa = 4.29KK41 pKa = 10.67ASSVSLAGFRR51 pKa = 11.84FSPLQEE57 pKa = 3.87TDD59 pKa = 2.97LTDD62 pKa = 3.17LHH64 pKa = 6.75RR65 pKa = 11.84FLMTLPEE72 pKa = 4.51DD73 pKa = 3.39SVAYY77 pKa = 8.46FNPHH81 pKa = 6.68AFTLPALRR89 pKa = 11.84RR90 pKa = 11.84LHH92 pKa = 6.58RR93 pKa = 11.84SGSFVMLGVRR103 pKa = 11.84QGDD106 pKa = 3.98TLVGYY111 pKa = 10.43HH112 pKa = 6.53FLRR115 pKa = 11.84CFASGRR121 pKa = 11.84CFHH124 pKa = 6.66GLVVSPSAQGRR135 pKa = 11.84GIGTAMWDD143 pKa = 3.39LGARR147 pKa = 11.84IATAAGLAMFATISEE162 pKa = 4.64HH163 pKa = 5.07NHH165 pKa = 6.4PSLTSCTRR173 pKa = 11.84GCHH176 pKa = 3.93TTIADD181 pKa = 4.51RR182 pKa = 11.84LPGSYY187 pKa = 10.77LLIRR191 pKa = 11.84CQPKK195 pKa = 9.42KK196 pKa = 10.32HH197 pKa = 6.08KK198 pKa = 10.57AA199 pKa = 3.21

Molecular weight: 22.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2703

0

2703

952895

31

3898

352.5

39.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.274 ± 0.042

1.262 ± 0.019

6.003 ± 0.034

6.139 ± 0.044

4.263 ± 0.029

7.041 ± 0.043

1.851 ± 0.02

6.818 ± 0.044

5.424 ± 0.041

8.986 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.021

4.612 ± 0.038

4.127 ± 0.029

2.944 ± 0.028

5.596 ± 0.044

6.656 ± 0.041

5.663 ± 0.035

6.512 ± 0.034

1.21 ± 0.019

3.926 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski