Pseudomonas phage phi-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Tunggulvirus; Pseudomonas virus f2

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2EBR5|D2EBR5_9CAUD Hypothetical phage protein OS=Pseudomonas phage phi-2 OX=693582 GN=SBWP25_0011 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 5.66LLRR5 pKa = 11.84RR6 pKa = 11.84MQHH9 pKa = 4.93VLEE12 pKa = 4.61ALLLVSINVHH22 pKa = 5.15VGGGAVRR29 pKa = 11.84DD30 pKa = 3.88MVLGLGKK37 pKa = 9.71PKK39 pKa = 10.8DD40 pKa = 3.92LDD42 pKa = 3.84VVMLAPDD49 pKa = 4.47DD50 pKa = 4.04DD51 pKa = 4.65QIRR54 pKa = 11.84QAVLVLGCIGYY65 pKa = 9.86KK66 pKa = 9.69PVSSFGLDD74 pKa = 3.69GGWSGHH80 pKa = 5.66QGQEE84 pKa = 3.36FHH86 pKa = 7.47ADD88 pKa = 3.03VEE90 pKa = 4.69YY91 pKa = 10.65AAEE94 pKa = 4.33NGDD97 pKa = 3.61GTFDD101 pKa = 3.46EE102 pKa = 4.8RR103 pKa = 11.84WRR105 pKa = 11.84VLIKK109 pKa = 10.32FVHH112 pKa = 5.37VVTGEE117 pKa = 3.97EE118 pKa = 4.09LDD120 pKa = 4.3LLCSHH125 pKa = 7.1TDD127 pKa = 3.5DD128 pKa = 3.64VDD130 pKa = 3.97EE131 pKa = 5.05LVGSYY136 pKa = 10.47DD137 pKa = 3.72YY138 pKa = 11.43NINQYY143 pKa = 10.18VLFPGDD149 pKa = 3.87SIATFLGDD157 pKa = 4.74DD158 pKa = 4.01AGLLMKK164 pKa = 10.78LRR166 pKa = 11.84DD167 pKa = 4.33DD168 pKa = 4.46GVCLTRR174 pKa = 11.84QHH176 pKa = 7.01KK177 pKa = 9.55MYY179 pKa = 10.67QKK181 pKa = 11.16ALAAGWNINEE191 pKa = 4.36VPAALPFF198 pKa = 4.43

Molecular weight: 21.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2EBQ6|D2EBQ6_9CAUD Hypothetical phage protein OS=Pseudomonas phage phi-2 OX=693582 GN=SBWP25_0002 PE=4 SV=1
MM1 pKa = 6.77TTTAQRR7 pKa = 11.84LRR9 pKa = 11.84AIDD12 pKa = 3.43KK13 pKa = 9.81KK14 pKa = 10.77ARR16 pKa = 11.84RR17 pKa = 11.84QMRR20 pKa = 11.84HH21 pKa = 4.71RR22 pKa = 11.84ARR24 pKa = 11.84KK25 pKa = 9.03EE26 pKa = 3.62GVTIEE31 pKa = 4.11GVITATTGTLRR42 pKa = 11.84DD43 pKa = 3.51VAQRR47 pKa = 11.84SVNVTQLYY55 pKa = 10.08GAAQRR60 pKa = 11.84DD61 pKa = 3.44MGKK64 pKa = 9.74RR65 pKa = 11.84LKK67 pKa = 10.53LGQAHH72 pKa = 7.58RR73 pKa = 11.84DD74 pKa = 3.25GDD76 pKa = 4.05VQAGKK81 pKa = 10.43ARR83 pKa = 11.84RR84 pKa = 11.84KK85 pKa = 10.32LNDD88 pKa = 3.23WEE90 pKa = 5.69LDD92 pKa = 3.65TKK94 pKa = 10.71PAGPATAWQPKK105 pKa = 7.72GGRR108 pKa = 11.84RR109 pKa = 11.84AVGKK113 pKa = 10.14CDD115 pKa = 3.46ADD117 pKa = 3.81AVSKK121 pKa = 10.82LNKK124 pKa = 9.65AKK126 pKa = 10.66RR127 pKa = 11.84GG128 pKa = 3.65

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

12862

79

1014

299.1

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.429 ± 0.593

1.158 ± 0.207

6.173 ± 0.225

5.652 ± 0.292

3.242 ± 0.172

7.697 ± 0.299

2.231 ± 0.219

3.965 ± 0.207

5.279 ± 0.317

8.723 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.184

3.872 ± 0.204

4.486 ± 0.292

4.253 ± 0.302

6.134 ± 0.278

5.24 ± 0.256

6.018 ± 0.405

7.238 ± 0.347

1.563 ± 0.154

2.908 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski