Amino acid dipepetide frequency for Chlamydomonas reinhardtii (Chlamydomonas smithii)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	51.442AlaAla: 51.442 ± 0.314	1.728AlaCys: 1.728 ± 0.016	6.071AlaAsp: 6.071 ± 0.029	7.669AlaGlu: 7.669 ± 0.066	2.612AlaPhe: 2.612 ± 0.017	19.292AlaGly: 19.292 ± 0.086	2.493AlaHis: 2.493 ± 0.018	2.433AlaIle: 2.433 ± 0.016	3.209AlaLys: 3.209 ± 0.03	11.797AlaLeu: 11.797 ± 0.054	2.513AlaMet: 2.513 ± 0.015	2.508AlaAsn: 2.508 ± 0.018	11.167AlaPro: 11.167 ± 0.085	5.185AlaGln: 5.185 ± 0.04	7.73AlaArg: 7.73 ± 0.04	11.252AlaSer: 11.252 ± 0.057	8.283AlaThr: 8.283 ± 0.047	10.66AlaVal: 10.66 ± 0.046	1.455AlaTrp: 1.455 ± 0.012	1.923AlaTyr: 1.923 ± 0.018	0.0AlaXaa: 0.0 ± 0.0
Cys	1.502CysAla: 1.502 ± 0.012	0.41CysCys: 0.41 ± 0.007	0.621CysAsp: 0.621 ± 0.008	0.534CysGlu: 0.534 ± 0.006	0.366CysPhe: 0.366 ± 0.006	1.529CysGly: 1.529 ± 0.013	0.264CysHis: 0.264 ± 0.005	0.346CysIle: 0.346 ± 0.005	0.358CysLys: 0.358 ± 0.005	1.186CysLeu: 1.186 ± 0.013	0.27CysMet: 0.27 ± 0.005	0.351CysAsn: 0.351 ± 0.006	0.809CysPro: 0.809 ± 0.011	0.381CysGln: 0.381 ± 0.007	0.943CysArg: 0.943 ± 0.01	1.16CysSer: 1.16 ± 0.012	0.7CysThr: 0.7 ± 0.01	0.949CysVal: 0.949 ± 0.01	0.242CysTrp: 0.242 ± 0.005	0.289CysTyr: 0.289 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	5.924AspAla: 5.924 ± 0.033	0.635AspCys: 0.635 ± 0.008	2.827AspAsp: 2.827 ± 0.027	2.77AspGlu: 2.77 ± 0.024	1.103AspPhe: 1.103 ± 0.012	5.031AspGly: 5.031 ± 0.026	0.634AspHis: 0.634 ± 0.008	1.153AspIle: 1.153 ± 0.011	1.263AspLys: 1.263 ± 0.018	3.549AspLeu: 3.549 ± 0.019	0.962AspMet: 0.962 ± 0.009	0.905AspAsn: 0.905 ± 0.01	2.608AspPro: 2.608 ± 0.02	1.108AspGln: 1.108 ± 0.01	2.163AspArg: 2.163 ± 0.02	2.806AspSer: 2.806 ± 0.018	2.087AspThr: 2.087 ± 0.015	3.23AspVal: 3.23 ± 0.017	0.651AspTrp: 0.651 ± 0.008	0.818AspTyr: 0.818 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	8.321GluAla: 8.321 ± 0.066	0.491GluCys: 0.491 ± 0.007	2.68GluAsp: 2.68 ± 0.026	4.956GluGlu: 4.956 ± 0.056	0.862GluPhe: 0.862 ± 0.009	4.357GluGly: 4.357 ± 0.029	0.944GluHis: 0.944 ± 0.01	0.834GluIle: 0.834 ± 0.011	1.245GluLys: 1.245 ± 0.017	4.961GluLeu: 4.961 ± 0.036	0.858GluMet: 0.858 ± 0.009	0.641GluAsn: 0.641 ± 0.008	2.947GluPro: 2.947 ± 0.063	2.632GluGln: 2.632 ± 0.025	3.337GluArg: 3.337 ± 0.027	2.109GluSer: 2.109 ± 0.016	1.426GluThr: 1.426 ± 0.013	3.415GluVal: 3.415 ± 0.022	0.623GluTrp: 0.623 ± 0.007	0.799GluTyr: 0.799 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	2.32PheAla: 2.32 ± 0.015	0.352PheCys: 0.352 ± 0.006	1.087PheAsp: 1.087 ± 0.011	1.007PheGlu: 1.007 ± 0.009	0.677PhePhe: 0.677 ± 0.009	2.099PheGly: 2.099 ± 0.016	0.419PheHis: 0.419 ± 0.006	0.649PheIle: 0.649 ± 0.009	0.717PheLys: 0.717 ± 0.008	1.802PheLeu: 1.802 ± 0.016	0.505PheMet: 0.505 ± 0.007	0.665PheAsn: 0.665 ± 0.01	1.039PhePro: 1.039 ± 0.012	0.653PheGln: 0.653 ± 0.008	1.208PheArg: 1.208 ± 0.011	1.522PheSer: 1.522 ± 0.014	1.216PheThr: 1.216 ± 0.013	1.418PheVal: 1.418 ± 0.014	0.301PheTrp: 0.301 ± 0.005	0.494PheTyr: 0.494 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	17.529GlyAla: 17.529 ± 0.082	1.461GlyCys: 1.461 ± 0.014	4.338GlyAsp: 4.338 ± 0.025	4.139GlyGlu: 4.139 ± 0.026	2.005GlyPhe: 2.005 ± 0.014	26.375GlyGly: 26.375 ± 0.209	1.946GlyHis: 1.946 ± 0.017	1.802GlyIle: 1.802 ± 0.012	2.376GlyLys: 2.376 ± 0.022	7.668GlyLeu: 7.668 ± 0.036	1.974GlyMet: 1.974 ± 0.019	2.089GlyAsn: 2.089 ± 0.022	6.252GlyPro: 6.252 ± 0.037	3.274GlyGln: 3.274 ± 0.02	6.731GlyArg: 6.731 ± 0.037	10.843GlySer: 10.843 ± 0.054	4.878GlyThr: 4.878 ± 0.029	6.183GlyVal: 6.183 ± 0.027	1.19GlyTrp: 1.19 ± 0.015	1.69GlyTyr: 1.69 ± 0.016	0.0GlyXaa: 0.0 ± 0.0
His	2.517HisAla: 2.517 ± 0.021	0.324HisCys: 0.324 ± 0.005	0.858HisAsp: 0.858 ± 0.008	0.837HisGlu: 0.837 ± 0.007	0.499HisPhe: 0.499 ± 0.006	2.1HisGly: 2.1 ± 0.018	1.1HisHis: 1.1 ± 0.018	0.507HisIle: 0.507 ± 0.007	0.519HisLys: 0.519 ± 0.007	1.895HisLeu: 1.895 ± 0.014	0.521HisMet: 0.521 ± 0.006	0.496HisAsn: 0.496 ± 0.006	1.574HisPro: 1.574 ± 0.014	1.289HisGln: 1.289 ± 0.02	1.352HisArg: 1.352 ± 0.012	1.522HisSer: 1.522 ± 0.014	1.107HisThr: 1.107 ± 0.01	1.464HisVal: 1.464 ± 0.012	0.28HisTrp: 0.28 ± 0.005	0.43HisTyr: 0.43 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	2.427IleAla: 2.427 ± 0.014	0.32IleCys: 0.32 ± 0.005	1.0IleAsp: 1.0 ± 0.011	0.928IleGlu: 0.928 ± 0.01	0.571IlePhe: 0.571 ± 0.009	1.513IleGly: 1.513 ± 0.01	0.419IleHis: 0.419 ± 0.006	0.739IleIle: 0.739 ± 0.01	0.856IleLys: 0.856 ± 0.011	1.569IleLeu: 1.569 ± 0.016	0.49IleMet: 0.49 ± 0.007	0.704IleAsn: 0.704 ± 0.01	1.106IlePro: 1.106 ± 0.012	0.701IleGln: 0.701 ± 0.008	1.246IleArg: 1.246 ± 0.011	1.561IleSer: 1.561 ± 0.013	1.364IleThr: 1.364 ± 0.014	1.28IleVal: 1.28 ± 0.013	0.232IleTrp: 0.232 ± 0.004	0.457IleTyr: 0.457 ± 0.007	0.0IleXaa: 0.0 ± 0.0
Lys	3.404LysAla: 3.404 ± 0.033	0.283LysCys: 0.283 ± 0.005	1.167LysAsp: 1.167 ± 0.015	1.588LysGlu: 1.588 ± 0.018	0.548LysPhe: 0.548 ± 0.008	1.895LysGly: 1.895 ± 0.019	0.52LysHis: 0.52 ± 0.007	0.586LysIle: 0.586 ± 0.009	1.295LysLys: 1.295 ± 0.02	2.297LysLeu: 2.297 ± 0.019	0.499LysMet: 0.499 ± 0.007	0.52LysAsn: 0.52 ± 0.009	1.662LysPro: 1.662 ± 0.016	1.249LysGln: 1.249 ± 0.014	1.823LysArg: 1.823 ± 0.017	1.385LysSer: 1.385 ± 0.013	1.035LysThr: 1.035 ± 0.011	1.558LysVal: 1.558 ± 0.015	0.286LysTrp: 0.286 ± 0.005	0.552LysTyr: 0.552 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	11.82LeuAla: 11.82 ± 0.054	1.205LeuCys: 1.205 ± 0.014	3.901LeuAsp: 3.901 ± 0.018	4.498LeuGlu: 4.498 ± 0.032	1.815LeuPhe: 1.815 ± 0.015	6.962LeuGly: 6.962 ± 0.032	2.152LeuHis: 2.152 ± 0.016	1.592LeuIle: 1.592 ± 0.015	2.217LeuLys: 2.217 ± 0.018	10.108LeuLeu: 10.108 ± 0.046	1.681LeuMet: 1.681 ± 0.013	1.677LeuAsn: 1.677 ± 0.015	7.006LeuPro: 7.006 ± 0.035	5.149LeuGln: 5.149 ± 0.036	6.478LeuArg: 6.478 ± 0.034	5.747LeuSer: 5.747 ± 0.031	4.087LeuThr: 4.087 ± 0.029	5.422LeuVal: 5.422 ± 0.029	1.068LeuTrp: 1.068 ± 0.01	1.571LeuTyr: 1.571 ± 0.013	0.0LeuXaa: 0.0 ± 0.0
Met	2.633MetAla: 2.633 ± 0.019	0.242MetCys: 0.242 ± 0.004	0.885MetAsp: 0.885 ± 0.008	1.004MetGlu: 1.004 ± 0.01	0.41MetPhe: 0.41 ± 0.005	1.641MetGly: 1.641 ± 0.017	0.428MetHis: 0.428 ± 0.007	0.341MetIle: 0.341 ± 0.005	0.434MetLys: 0.434 ± 0.006	1.765MetLeu: 1.765 ± 0.012	0.464MetMet: 0.464 ± 0.009	0.348MetAsn: 0.348 ± 0.007	1.348MetPro: 1.348 ± 0.013	0.998MetGln: 0.998 ± 0.011	1.235MetArg: 1.235 ± 0.01	1.327MetSer: 1.327 ± 0.011	0.85MetThr: 0.85 ± 0.008	1.05MetVal: 1.05 ± 0.012	0.224MetTrp: 0.224 ± 0.004	0.38MetTyr: 0.38 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.487AsnAla: 2.487 ± 0.017	0.317AsnCys: 0.317 ± 0.006	0.827AsnAsp: 0.827 ± 0.01	0.744AsnGlu: 0.744 ± 0.01	0.534AsnPhe: 0.534 ± 0.008	2.31AsnGly: 2.31 ± 0.019	0.373AsnHis: 0.373 ± 0.006	0.675AsnIle: 0.675 ± 0.01	0.674AsnLys: 0.674 ± 0.01	1.597AsnLeu: 1.597 ± 0.014	0.443AsnMet: 0.443 ± 0.006	0.717AsnAsn: 0.717 ± 0.012	1.254AsnPro: 1.254 ± 0.012	0.601AsnGln: 0.601 ± 0.007	1.067AsnArg: 1.067 ± 0.011	1.675AsnSer: 1.675 ± 0.016	1.267AsnThr: 1.267 ± 0.014	1.273AsnVal: 1.273 ± 0.015	0.253AsnTrp: 0.253 ± 0.005	0.429AsnTyr: 0.429 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	12.714ProAla: 12.714 ± 0.087	0.717ProCys: 0.717 ± 0.009	2.755ProAsp: 2.755 ± 0.019	3.292ProGlu: 3.292 ± 0.063	1.262ProPhe: 1.262 ± 0.012	7.187ProGly: 7.187 ± 0.049	1.837ProHis: 1.837 ± 0.018	1.092ProIle: 1.092 ± 0.01	1.344ProLys: 1.344 ± 0.014	5.971ProLeu: 5.971 ± 0.032	0.993ProMet: 0.993 ± 0.011	1.187ProAsn: 1.187 ± 0.012	12.773ProPro: 12.773 ± 0.254	3.726ProGln: 3.726 ± 0.036	4.013ProArg: 4.013 ± 0.022	7.271ProSer: 7.271 ± 0.175	3.262ProThr: 3.262 ± 0.022	3.997ProVal: 3.997 ± 0.027	0.728ProTrp: 0.728 ± 0.007	1.197ProTyr: 1.197 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	5.65GlnAla: 5.65 ± 0.039	0.392GlnCys: 0.392 ± 0.006	1.322GlnAsp: 1.322 ± 0.012	2.326GlnGlu: 2.326 ± 0.021	0.627GlnPhe: 0.627 ± 0.008	3.048GlnGly: 3.048 ± 0.022	1.755GlnHis: 1.755 ± 0.022	0.64GlnIle: 0.64 ± 0.008	0.82GlnLys: 0.82 ± 0.01	5.191GlnLeu: 5.191 ± 0.031	0.718GlnMet: 0.718 ± 0.008	0.543GlnAsn: 0.543 ± 0.008	4.533GlnPro: 4.533 ± 0.041	8.737GlnGln: 8.737 ± 0.113	3.378GlnArg: 3.378 ± 0.024	2.019GlnSer: 2.019 ± 0.014	1.288GlnThr: 1.288 ± 0.011	2.304GlnVal: 2.304 ± 0.015	0.475GlnTrp: 0.475 ± 0.007	0.696GlnTyr: 0.696 ± 0.009	0.0GlnXaa: 0.0 ± 0.0
Arg	7.684ArgAla: 7.684 ± 0.041	0.969ArgCys: 0.969 ± 0.009	2.59ArgAsp: 2.59 ± 0.02	3.068ArgGlu: 3.068 ± 0.026	1.333ArgPhe: 1.333 ± 0.011	5.845ArgGly: 5.845 ± 0.036	1.624ArgHis: 1.624 ± 0.013	1.345ArgIle: 1.345 ± 0.011	1.718ArgLys: 1.718 ± 0.017	6.543ArgLeu: 6.543 ± 0.033	1.222ArgMet: 1.222 ± 0.011	1.26ArgAsn: 1.26 ± 0.012	4.371ArgPro: 4.371 ± 0.024	3.314ArgGln: 3.314 ± 0.02	6.337ArgArg: 6.337 ± 0.036	4.654ArgSer: 4.654 ± 0.029	2.726ArgThr: 2.726 ± 0.015	3.699ArgVal: 3.699 ± 0.018	0.864ArgTrp: 0.864 ± 0.009	1.134ArgTyr: 1.134 ± 0.011	0.0ArgXaa: 0.0 ± 0.0
Ser	10.844SerAla: 10.844 ± 0.055	1.09SerCys: 1.09 ± 0.011	2.933SerAsp: 2.933 ± 0.017	2.598SerGlu: 2.598 ± 0.019	1.577SerPhe: 1.577 ± 0.014	10.433SerGly: 10.433 ± 0.056	1.444SerHis: 1.444 ± 0.012	1.564SerIle: 1.564 ± 0.013	1.635SerLys: 1.635 ± 0.015	5.687SerLeu: 5.687 ± 0.025	1.249SerMet: 1.249 ± 0.01	1.793SerAsn: 1.793 ± 0.016	7.256SerPro: 7.256 ± 0.189	2.375SerGln: 2.375 ± 0.016	4.73SerArg: 4.73 ± 0.029	10.326SerSer: 10.326 ± 0.079	4.191SerThr: 4.191 ± 0.025	3.863SerVal: 3.863 ± 0.02	0.83SerTrp: 0.83 ± 0.01	1.303SerTyr: 1.303 ± 0.016	0.0SerXaa: 0.0 ± 0.0
Thr	9.116ThrAla: 9.116 ± 0.053	0.756ThrCys: 0.756 ± 0.011	1.888ThrAsp: 1.888 ± 0.014	1.768ThrGlu: 1.768 ± 0.014	1.089ThrPhe: 1.089 ± 0.012	4.994ThrGly: 4.994 ± 0.029	0.965ThrHis: 0.965 ± 0.009	1.091ThrIle: 1.091 ± 0.012	1.0ThrLys: 1.0 ± 0.012	3.693ThrLeu: 3.693 ± 0.024	0.709ThrMet: 0.709 ± 0.009	1.015ThrAsn: 1.015 ± 0.012	3.767ThrPro: 3.767 ± 0.029	1.553ThrGln: 1.553 ± 0.013	2.427ThrArg: 2.427 ± 0.013	4.28ThrSer: 4.28 ± 0.022	3.147ThrThr: 3.147 ± 0.027	2.789ThrVal: 2.789 ± 0.021	0.586ThrTrp: 0.586 ± 0.007	0.913ThrTyr: 0.913 ± 0.011	0.0ThrXaa: 0.0 ± 0.0
Val	10.034ValAla: 10.034 ± 0.034	0.982ValCys: 0.982 ± 0.011	2.834ValAsp: 2.834 ± 0.016	3.271ValGlu: 3.271 ± 0.022	1.478ValPhe: 1.478 ± 0.015	5.187ValGly: 5.187 ± 0.025	1.214ValHis: 1.214 ± 0.011	1.37ValIle: 1.37 ± 0.014	1.614ValLys: 1.614 ± 0.016	6.062ValLeu: 6.062 ± 0.029	1.289ValMet: 1.289 ± 0.011	1.241ValAsn: 1.241 ± 0.012	4.261ValPro: 4.261 ± 0.034	2.304ValGln: 2.304 ± 0.015	3.898ValArg: 3.898 ± 0.023	4.229ValSer: 4.229 ± 0.022	3.176ValThr: 3.176 ± 0.021	4.934ValVal: 4.934 ± 0.031	0.834ValTrp: 0.834 ± 0.009	1.162ValTyr: 1.162 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	1.389TrpAla: 1.389 ± 0.011	0.199TrpCys: 0.199 ± 0.004	0.59TrpAsp: 0.59 ± 0.007	0.613TrpGlu: 0.613 ± 0.006	0.278TrpPhe: 0.278 ± 0.005	0.958TrpGly: 0.958 ± 0.011	0.278TrpHis: 0.278 ± 0.005	0.24TrpIle: 0.24 ± 0.005	0.29TrpLys: 0.29 ± 0.005	1.293TrpLeu: 1.293 ± 0.011	0.245TrpMet: 0.245 ± 0.005	0.277TrpAsn: 0.277 ± 0.006	0.682TrpPro: 0.682 ± 0.008	0.613TrpGln: 0.613 ± 0.007	1.149TrpArg: 1.149 ± 0.012	0.804TrpSer: 0.804 ± 0.01	0.501TrpThr: 0.501 ± 0.007	0.721TrpVal: 0.721 ± 0.011	0.232TrpTrp: 0.232 ± 0.005	0.244TrpTyr: 0.244 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.665TyrAla: 1.665 ± 0.013	0.336TyrCys: 0.336 ± 0.006	1.053TyrAsp: 1.053 ± 0.01	0.829TyrGlu: 0.829 ± 0.008	0.504TyrPhe: 0.504 ± 0.007	1.986TyrGly: 1.986 ± 0.018	0.381TyrHis: 0.381 ± 0.006	0.485TyrIle: 0.485 ± 0.006	0.569TyrLys: 0.569 ± 0.008	1.521TyrLeu: 1.521 ± 0.013	0.406TyrMet: 0.406 ± 0.007	0.58TyrAsn: 0.58 ± 0.008	0.904TyrPro: 0.904 ± 0.023	0.62TyrGln: 0.62 ± 0.008	1.055TyrArg: 1.055 ± 0.011	1.231TyrSer: 1.231 ± 0.01	0.954TyrThr: 0.954 ± 0.01	1.164TyrVal: 1.164 ± 0.012	0.241TyrTrp: 0.241 ± 0.005	0.479TyrTyr: 0.479 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.022XaaXaa: 0.022 ± 0.006

Statistics based on 18832 proteins (14029357 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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