Bacteroides sp. CAG:754

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R6DIU5|R6DIU5_9BACE TPR_REGION domain-containing protein OS=Bacteroides sp. CAG:754 OX=1262750 GN=BN772_00208 PE=4 SV=1
MM1 pKa = 7.67WKK3 pKa = 10.32FKK5 pKa = 10.37FGAWAVVLMMTALSFSACSDD25 pKa = 3.96DD26 pKa = 5.9DD27 pKa = 5.11DD28 pKa = 6.57DD29 pKa = 5.02PALVPPSNITEE40 pKa = 4.2ALKK43 pKa = 10.48QLYY46 pKa = 8.51PAAQNIEE53 pKa = 3.84WEE55 pKa = 4.47MKK57 pKa = 9.48GDD59 pKa = 4.09YY60 pKa = 10.28YY61 pKa = 11.67VADD64 pKa = 3.66CWVTGDD70 pKa = 4.82EE71 pKa = 4.31LDD73 pKa = 3.44VWFDD77 pKa = 4.11ANANWVMTEE86 pKa = 4.01NEE88 pKa = 4.68LNSIDD93 pKa = 3.83QLVPAVYY100 pKa = 8.52TAFQNSDD107 pKa = 2.85YY108 pKa = 10.49SAWVVTDD115 pKa = 3.8VYY117 pKa = 11.62VLTYY121 pKa = 7.88PQNMTEE127 pKa = 4.63SVIQVKK133 pKa = 10.34LGSQRR138 pKa = 11.84YY139 pKa = 8.37SLYY142 pKa = 10.29FSQEE146 pKa = 3.14GGLLHH151 pKa = 7.08KK152 pKa = 10.44KK153 pKa = 10.31DD154 pKa = 3.91ISNGDD159 pKa = 3.54DD160 pKa = 3.75TNWPPTEE167 pKa = 4.08NNN169 pKa = 3.11

Molecular weight:
19.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R6DFJ4|R6DFJ4_9BACE OMP_b-brl domain-containing protein OS=Bacteroides sp. CAG:754 OX=1262750 GN=BN772_02114 PE=4 SV=1
MM1 pKa = 7.75HH2 pKa = 7.48IPSQTYY8 pKa = 9.15RR9 pKa = 11.84PRR11 pKa = 11.84IPEE14 pKa = 3.95TIVRR18 pKa = 11.84SEE20 pKa = 4.01HH21 pKa = 6.34RR22 pKa = 11.84CTIVTIIEE30 pKa = 4.01IQQITVIIRR39 pKa = 11.84ISPIDD44 pKa = 3.57IEE46 pKa = 4.3TGRR49 pKa = 11.84TFLILTVFRR58 pKa = 11.84FSAIGIAIGKK68 pKa = 8.93GRR70 pKa = 11.84SSKK73 pKa = 10.95LIFRR77 pKa = 11.84KK78 pKa = 8.04TICRR82 pKa = 11.84IAVHH86 pKa = 6.83CILITTEE93 pKa = 3.07IKK95 pKa = 10.15ARR97 pKa = 11.84YY98 pKa = 8.27GIHH101 pKa = 6.88HH102 pKa = 6.67MFVCEE107 pKa = 4.01KK108 pKa = 8.25FTKK111 pKa = 10.13RR112 pKa = 11.84RR113 pKa = 11.84GKK115 pKa = 10.08IPFIYY120 pKa = 8.43HH121 pKa = 5.65TLCQRR126 pKa = 11.84MSKK129 pKa = 10.27QSFLIFSHH137 pKa = 6.98IIKK140 pKa = 10.52LL141 pKa = 3.73

Molecular weight:
16.45 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4054

0

4054

1597693

29

1986

394.1

44.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.849 ± 0.036

1.192 ± 0.014

5.557 ± 0.023

6.491 ± 0.034

4.584 ± 0.026

6.964 ± 0.031

1.776 ± 0.014

6.759 ± 0.035

6.619 ± 0.028

8.925 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.016

5.363 ± 0.035

3.741 ± 0.02

3.441 ± 0.019

4.476 ± 0.023

6.287 ± 0.029

5.789 ± 0.027

6.371 ± 0.029

1.392 ± 0.014

4.713 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski