Tissierella sp. P1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Tissierellaceae; Tissierella; unclassified Tissierella

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A265Q181|A0A265Q181_9FIRM ATP-dependent zinc metalloprotease FtsH OS=Tissierella sp. P1 OX=1280483 GN=ftsH PE=3 SV=1
MM1 pKa = 7.15ATISGRR7 pKa = 11.84VVFDD11 pKa = 3.76RR12 pKa = 11.84DD13 pKa = 3.01RR14 pKa = 11.84SATINAGDD22 pKa = 3.69SGLANVPVVLQNTMTDD38 pKa = 2.84MRR40 pKa = 11.84LVVLTDD46 pKa = 3.24NNGNYY51 pKa = 10.53SFINVPNGSYY61 pKa = 10.43RR62 pKa = 11.84IVEE65 pKa = 4.4SYY67 pKa = 7.83GTPGGVATPGDD78 pKa = 4.12FNTAVVGPIPVGEE91 pKa = 4.68DD92 pKa = 3.47PPISFAPNPPSGSTNLDD109 pKa = 3.3SLTPDD114 pKa = 3.28TLLVTVSGFDD124 pKa = 3.36LTNQNFLDD132 pKa = 4.55GPVIYY137 pKa = 9.94TPIEE141 pKa = 4.5NILDD145 pKa = 3.84PCVSVSDD152 pKa = 3.61INLINVADD160 pKa = 3.97NGTFGSFPAGTPANTGAPVEE180 pKa = 4.5PYY182 pKa = 10.34PGVTPDD188 pKa = 3.43FTYY191 pKa = 11.09VLPDD195 pKa = 3.48PTDD198 pKa = 3.38YY199 pKa = 11.39TPFDD203 pKa = 3.46GEE205 pKa = 4.34YY206 pKa = 9.04TVQNIMTNALSNTIGAWWRR225 pKa = 11.84IADD228 pKa = 3.69HH229 pKa = 5.57TTGNEE234 pKa = 3.97TGRR237 pKa = 11.84MMVVNGFDD245 pKa = 3.25PGAVFFRR252 pKa = 11.84DD253 pKa = 3.66VVSVQPNTNYY263 pKa = 10.51LFSSWILNLFKK274 pKa = 10.74VTGYY278 pKa = 9.51PNPEE282 pKa = 3.96LGVRR286 pKa = 11.84ILDD289 pKa = 3.59QNGNPIYY296 pKa = 10.53SATLGAQIPVNTNTPEE312 pKa = 3.66WKK314 pKa = 10.34EE315 pKa = 3.48IGTVINSRR323 pKa = 11.84NNTSLTVEE331 pKa = 4.56FLSEE335 pKa = 4.04GPAAIGNDD343 pKa = 3.5YY344 pKa = 11.0AIDD347 pKa = 5.11DD348 pKa = 3.55ISLRR352 pKa = 11.84EE353 pKa = 3.79IQIPEE358 pKa = 3.96FTPVKK363 pKa = 9.7TVSTPTANIGDD374 pKa = 3.73IVTYY378 pKa = 9.77TVTLTNTCTSPLTNVFFQDD397 pKa = 3.39IVPNGLSFVPGSVTVNGSQDD417 pKa = 2.61ITLNPNVGFNVPDD430 pKa = 3.42IPGGSTATITFDD442 pKa = 3.56ALVDD446 pKa = 3.88NVPTPNPTLNTAMMSYY462 pKa = 10.28FYY464 pKa = 10.65TPVEE468 pKa = 4.05GGIEE472 pKa = 3.84NNYY475 pKa = 8.3TVNSNVVPLDD485 pKa = 3.19IAASADD491 pKa = 3.33VSVEE495 pKa = 4.17KK496 pKa = 10.17TSSPDD501 pKa = 3.41PVISGQVLQYY511 pKa = 10.12TIIVSNAGPSAAEE524 pKa = 3.63NVVLHH529 pKa = 6.83DD530 pKa = 5.39IIPPEE535 pKa = 3.57ITGAEE540 pKa = 4.19FSTDD544 pKa = 2.86GGATFSPWTGSYY556 pKa = 10.8DD557 pKa = 3.86IGTLLNGEE565 pKa = 4.31TRR567 pKa = 11.84TILIRR572 pKa = 11.84GTVSPTATGTITNTANVTSTTPDD595 pKa = 3.72PNPDD599 pKa = 3.48NNTSTTTTEE608 pKa = 3.83VNALADD614 pKa = 3.33IGVVKK619 pKa = 9.89TGTPNPVMSGEE630 pKa = 4.2TLTYY634 pKa = 10.02TIDD637 pKa = 3.34VSNFGPSDD645 pKa = 3.57AEE647 pKa = 4.09NVVLTDD653 pKa = 5.46AIPAEE658 pKa = 3.94ITGAEE663 pKa = 4.24FSTDD667 pKa = 2.89GGVTFSPWTGSYY679 pKa = 11.03NIGTLLNGEE688 pKa = 4.31TRR690 pKa = 11.84TILIRR695 pKa = 11.84GTVAPMAPGFITNTANVTSTTPDD718 pKa = 3.69PNPSNNTSTAVIEE731 pKa = 4.56VNQTTPVADD740 pKa = 3.29VGIFKK745 pKa = 10.64SVEE748 pKa = 4.03TNPIIPGGTVIYY760 pKa = 9.3PIEE763 pKa = 4.15VSNLGPSNAEE773 pKa = 3.7NVVLTDD779 pKa = 5.46AIPAEE784 pKa = 3.94ITGAEE789 pKa = 4.24FSTDD793 pKa = 2.58GGVTFNPWTGSYY805 pKa = 11.0NIGTLLNGEE814 pKa = 4.31TRR816 pKa = 11.84TILIRR821 pKa = 11.84GTVSPSATGIITNTANVTSTTPDD844 pKa = 3.58PNPP847 pKa = 3.47

Molecular weight: 88.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A265QAA2|A0A265QAA2_9FIRM Oligopeptide ABC transporter ATP-binding protein OppF OS=Tissierella sp. P1 OX=1280483 GN=CIW83_03920 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.23KK3 pKa = 10.44NKK5 pKa = 9.64IKK7 pKa = 10.59ILGLSIVLLVAMAGCRR23 pKa = 11.84PANTNMRR30 pKa = 11.84NMSTQTRR37 pKa = 11.84LYY39 pKa = 10.84NDD41 pKa = 3.42NLNNRR46 pKa = 11.84WMTNTPYY53 pKa = 9.63NTRR56 pKa = 11.84DD57 pKa = 3.44RR58 pKa = 11.84LNTNLNNGMVRR69 pKa = 11.84DD70 pKa = 3.98NTNLNNNALRR80 pKa = 11.84NTPMTYY86 pKa = 10.75DD87 pKa = 3.32SLRR90 pKa = 11.84GNNLVPGTTDD100 pKa = 2.95NGNIANNNLSTRR112 pKa = 11.84ATAIAKK118 pKa = 9.59RR119 pKa = 11.84VAEE122 pKa = 4.05LPEE125 pKa = 3.99VKK127 pKa = 9.96GASVIVHH134 pKa = 6.68GNTALWDD141 pKa = 3.82VNN143 pKa = 3.67

Molecular weight: 15.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3981

0

3981

1147079

20

1815

288.1

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.428 ± 0.045

0.904 ± 0.012

5.673 ± 0.032

7.777 ± 0.046

4.331 ± 0.028

6.604 ± 0.045

1.434 ± 0.013

10.508 ± 0.047

8.212 ± 0.038

9.333 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.019

5.902 ± 0.03

2.984 ± 0.024

2.389 ± 0.019

3.696 ± 0.025

6.153 ± 0.028

4.916 ± 0.03

6.101 ± 0.034

0.756 ± 0.013

4.162 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski