Sphingobium phage Lacusarx

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lacusarxvirus; Sphingobium virus Lacusarx

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6DX16|A0A1W6DX16_9CAUD DNA ligase (NAD(+)) OS=Sphingobium phage Lacusarx OX=1980139 GN=ligA PE=3 SV=1
MM1 pKa = 6.97SQIIIAVSSDD11 pKa = 2.69WTGLYY16 pKa = 10.69LNEE19 pKa = 4.38HH20 pKa = 6.56LVDD23 pKa = 3.62QGHH26 pKa = 7.35DD27 pKa = 2.94ISLFSALTGIIGYY40 pKa = 9.04EE41 pKa = 3.71IEE43 pKa = 4.35SVEE46 pKa = 4.06EE47 pKa = 4.28RR48 pKa = 11.84EE49 pKa = 4.64LHH51 pKa = 5.58PVWINLHH58 pKa = 5.6GQLPSNLNDD67 pKa = 3.82VVWDD71 pKa = 4.18DD72 pKa = 5.24DD73 pKa = 4.12DD74 pKa = 4.08WSSSDD79 pKa = 5.05DD80 pKa = 4.05FSDD83 pKa = 5.39DD84 pKa = 3.95EE85 pKa = 5.21PDD87 pKa = 3.02

Molecular weight: 9.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6DXJ9|A0A1W6DXJ9_9CAUD Uncharacterized protein OS=Sphingobium phage Lacusarx OX=1980139 GN=LAV_00117 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.6KK3 pKa = 9.13MFVMAAAVASMLVAACDD20 pKa = 3.94RR21 pKa = 11.84QPSAAEE27 pKa = 3.52RR28 pKa = 11.84CMEE31 pKa = 4.21AKK33 pKa = 10.39NPQEE37 pKa = 4.67CYY39 pKa = 9.47QVANAGGDD47 pKa = 3.38INDD50 pKa = 3.79YY51 pKa = 11.05LIGGLGGYY59 pKa = 8.33MLAKK63 pKa = 10.12VMSGGRR69 pKa = 11.84QQTVIVQNPNYY80 pKa = 9.33HH81 pKa = 5.64GPRR84 pKa = 11.84RR85 pKa = 11.84YY86 pKa = 9.51VPSYY90 pKa = 9.21ASSRR94 pKa = 11.84QRR96 pKa = 11.84VLNGQVKK103 pKa = 7.87TVSTTTTKK111 pKa = 10.58RR112 pKa = 11.84GLFGSKK118 pKa = 6.77TTTTTKK124 pKa = 10.47TFGSKK129 pKa = 9.59PASSWGSSYY138 pKa = 11.43SKK140 pKa = 10.27PSKK143 pKa = 10.31SWGSSSRR150 pKa = 11.84SFSSSRR156 pKa = 11.84SSFSSRR162 pKa = 11.84SSFRR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 3.07

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

196

0

196

37336

32

1753

190.5

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.389 ± 0.264

1.031 ± 0.08

6.656 ± 0.179

6.458 ± 0.239

3.916 ± 0.127

7.941 ± 0.301

2.188 ± 0.153

5.641 ± 0.137

4.995 ± 0.197

7.845 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.105

3.364 ± 0.14

4.778 ± 0.164

2.973 ± 0.12

6.08 ± 0.204

5.721 ± 0.216

6.061 ± 0.222

6.425 ± 0.2

1.661 ± 0.095

3.056 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski