Bacteroides sp. CAG:927

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5B8B5|R5B8B5_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:927 OX=1262753 GN=BN813_01011 PE=4 SV=1
MM1 pKa = 7.16SQTIIRR7 pKa = 11.84IPDD10 pKa = 3.75LASLPQAAEE19 pKa = 3.9QFIDD23 pKa = 4.31AMDD26 pKa = 4.17SYY28 pKa = 11.67TVFAFNGPMGAGKK41 pKa = 7.44TTFIRR46 pKa = 11.84EE47 pKa = 4.13LCSALGVDD55 pKa = 3.92SDD57 pKa = 4.8YY58 pKa = 11.21ATSPSFSIINEE69 pKa = 4.02YY70 pKa = 10.36RR71 pKa = 11.84SSTTAEE77 pKa = 4.48LIYY80 pKa = 10.96HH81 pKa = 6.61FDD83 pKa = 3.82LYY85 pKa = 10.84RR86 pKa = 11.84LEE88 pKa = 5.36SIDD91 pKa = 3.56EE92 pKa = 4.25AFDD95 pKa = 3.6IGVEE99 pKa = 4.25DD100 pKa = 4.47YY101 pKa = 10.74FDD103 pKa = 4.31SGALCFIEE111 pKa = 4.34WPEE114 pKa = 4.37RR115 pKa = 11.84IADD118 pKa = 3.94ILPDD122 pKa = 3.46DD123 pKa = 4.13TVQVNITVDD132 pKa = 3.43PDD134 pKa = 3.28TDD136 pKa = 3.28EE137 pKa = 4.01RR138 pKa = 11.84TITINTPEE146 pKa = 3.88NDD148 pKa = 3.17

Molecular weight: 16.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5B0F3|R5B0F3_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:927 OX=1262753 GN=BN813_01907 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.36KK6 pKa = 9.96RR7 pKa = 11.84KK8 pKa = 6.44GHH10 pKa = 6.2KK11 pKa = 9.14MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2231

0

2231

770787

29

2489

345.5

38.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.252 ± 0.054

1.307 ± 0.021

5.637 ± 0.036

5.791 ± 0.045

4.148 ± 0.034

6.934 ± 0.046

2.126 ± 0.026

6.819 ± 0.046

5.47 ± 0.049

8.898 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.022

4.851 ± 0.048

4.193 ± 0.032

3.147 ± 0.03

4.985 ± 0.038

6.467 ± 0.045

5.808 ± 0.039

6.961 ± 0.044

1.217 ± 0.02

4.011 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski